PTCRA (NM_001243169) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	PT-ALPHA; PTA
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331963 representing NM_001243169. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGCCGGTACATGGCTGCTACTTCTCCTGGCCCTTGGGTGTCCAGCCCTACCCACAGGTCCTGTTTCC TTCCCTTCCTCACCAGAGGCTGCCACAACAGGCCCCATCTGGTTCTCAGCCGGCAATGGCAGTGCACTG GATGCCTTCACCTATGGCCCTTCCCCAGCAACGGATGGCACCTGGACCAACTTGGCCCATCTCTCCCTG CCTTCTGAGGAGCTGGCATCCTGGGAGCCTTTGGTCTGCCACACTGGGCCTGGGGCTGAGGGTCACAGC AGGAGTACACAGCCCATGCATCTGTCAGGAGAGGCTTCTACAGCCAGGACCTGCCCCCAGGAGCCTCTC AGGGGGACACCGGGTGGGGCGCTGTGGCTGGGGGTCCTGCGGCTGCTGCTCTTCAAGCTGCTGCTGTTT GACCTGCTCCTGACCTGCAGCTGCCTGTGCGACCCCGCGGGCCCGCTGCCTTCCCCCGCAACCACCACC CGCCTGCGAGCCCTCGGCTCCCATCGACTGCACCCGGCCACGGAGACTGGGGGACGAGAGGCCACCAGC TCACCCAGACCCCAGCCTCGGGACCGCCGCTGGGGTGACACCCCTCCGGGTCGGAAGCCCGGGAGCCCA GTATGGGGGGAAGGGTCTTACCTCAGCAGTTACCCCACTTGCCCAGCACAGGCCTGGTGCTCAAGATCT GCCCTCAGGGCTCCTTCCTCCAGTCTTGGAGCATTTTTTGCAGGTGACCTGCCTCCTCCTCTGCAGGCT GGAGCTGCCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001243169
Insert Size	771 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001243169.1
RefSeq Size	1022 bp
RefSeq ORF	771 bp
Locus ID	171558
UniProt ID	Q6ISU1
Protein Families	Transmembrane
Protein Pathways	Notch signaling pathway
MW	26.6 kDa
Gene Summary	The protein encoded by this gene is a single-pass type I membrane protein that is found in immmature but not mature T-cells. Along with TCRB and CD3 complex, the encoded protein forms the pre-T-cell receptor complex, which regulates early T-cell development. Four transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011] Transcript Variant: This variant (3) uses alternate in-frame splice junctions at the 5' ends of two exons and contains an alternate in-frame exon compared to variant 1. The resulting isoform (3) contains an alternate internal segment but lacks two others compared to isoform 1.

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