NDRG4 (NM_001242835) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	BDM1; SMAP-8; SMAP8
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331893 representing NM_001242835. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGCCGGAGTGCTGGGATGGGGAACATGACATCGAGACACCCTACGGCCTTCTGCATGTAGTGATCCGG GGCTCCCCCAAGGGGAACCGCCCAGCCATCCTCACCTACCATGATGTGGGCCTCAACCACAAACTATGC TTCAACACCTTCTTCAACTTCGAGGACATGCAGGAGATCACCAAGCACTTTGTGGTGTGTCACGTGGAT GCCCCTGGACAACAGGTGGGGGCGTCGCAGTTTCCTCAGGGGTACCAGTTCCCCTCCATGGAGCAGCTG GCTGCCATGCTCCCCAGCGTGGTGCAGCATTTCGGGTTCAAGTATGTGATTGGCATCGGAGTGGGCGCC GGAGCCTATGTGCTGGCCAAGTTTGCACTCATCTTCCCCGACCTGGTGGAGGGGCTGGTGCTGGTGAAC ATCGACCCCAATGGCAAAGGCTGGATAGACTGGGCTGCCACCAAGCTCTCCGGCCTAACTAGCACTTTA CCCGACACGGTGCTCTCCCACCTCTTCAGCCAGGAGGAGCTGGTGAACAACACAGAGTTGGTGCAGAGC TACCGGCAGCAGATTGGGAACGTGGTGAACCAGGCCAACCTGCAGCTCTTCTGGAACATGTACAACAGC CGCAGAGACCTGGACATTAACCGGCCTGGAACGGTGCCCAATGCCAAGACGCTCCGCTGCCCCGTGATG CTGGTGGTTGGGGATAATGCACCCGCTGAGGACGGGGTGGTGGAGTGCAACTCCAAACTGGACCCGACC ACTACGACCTTCCTGAAGATGGCAGACTCTGGAGGGCTGCCCCAGGTCACACAGCCAGGGAAGCTGACT GAAGCCTTCAAATACTTCCTGCAAGGCATGGGCTACATTGCGTACTTGAAGGACCGAAGGCTGAGTGGA GGAGCAGTGCCCTCAGCCAGCATGACCCGCCTGGCACGCTCCCGCACTGCATCCCTCACCAGTGCCAGC TCGGTGGATGGCAGCCGCCCACAGGCCTGCACCCACTCAGAGAGCAGCGAGGGGCTGGGCCAGGTCAAC CACACCATGGAGGTGTCCTGTTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001242835
Insert Size	1059 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001242835.1
RefSeq Size	3229 bp
RefSeq ORF	1059 bp
Locus ID	65009
UniProt ID	Q9ULP0
MW	38.5 kDa
Gene Summary	This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein that is required for cell cycle progression and survival in primary astrocytes and may be involved in the regulation of mitogenic signalling in vascular smooth muscles cells. Alternative splicing results in multiple transcripts encoding different isoforms.[provided by RefSeq, Jun 2011] Transcript Variant: This variant (6) has multiple differences at the 5' end which result in the use of a distinct translation initiation codon and an additional exon in the 3' coding region, compared to variant 2. The encoded protein (isoform 6; also known as NDRG4-Bvar) has a shorter and distinct N-terminus and longer C-terminus, compared to isoform 2.

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