BRDT (NM_001242807) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	BRD6; CT9; SPGF21
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331872 representing NM_001242807. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGTCTCTGCCAAGTCGACAAACAGCTATTATTGTTAACCCTCCTCCACCAGAATATATAAATACTAAG AAAAATGGGCGATTGACAAATCAACTTCAGTATCTACAAAAAGTTGTCCTAAAGGATTTATGGAAGCAT AGTTTTTCATGGCCCTTTCAACGTCCTGTGGATGCTGTGAAACTACAGTTGCCTCCTGGAGATGACATT GTTCTTATGGCACAAGCTCTAGAGAAGCTGTTTATGCAGAAATTATCTCAGATGCCACAAGAAGAGCAA GTTGTGGGTGTTAAGGAAAGAATCAAGAAAGGCACTCAACAGAATATAGCTGTTTCTTCTGCTAAAGAA AAATCATCACCCAGCGCAACAGAAAAAGTATTTAAGCAGCAAGAAATTCCTTCTGTATTTCCTAAGACA TCTATTTCTCCCTTGAACGTGGTACAGGGAGCTTCAGTCAACTCCAGTTCACAAACTGCGGCCCAAGTT ACAAAAGGTGTGAAGAGGAAAGCAGATACAACAACTCCTGCAACTTCAGCAGTTAAAGCAAGTAGTGAA TTTTCTCCAACATTCACAGAAAAATCAGTGGCACTGCCACCTATAAAAGAAAATATGCCAAAGAATGTT TTGCCAGATTCTCAGCAACAATATAATGTTGTGAAGACTGTTAAAGTAACTGAACAATTAAGGCACTGT AGTGAGATTCTTAAAGAAATGCTTGCAAAGAAACATTTTTCATATGCATGGCCCTTTTATAATCCTGTT GACGTTAATGCTTTGGGACTCCATAACTACTATGACGTTGTCAAAAATCCGATGGATCTTGGAACTATT AAGGAGAAAATGGATAACCAAGAATATAAGGATGCATACAAATTTGCGGCAGATGTTAGATTAATGTTC ATGAATTGCTACAAGTACAATCCTCCAGATCACGAAGTTGTGACAATGGCAAGAATGCTTCAGGATGTT TTCGAAACGCATTTTTCAAAGATCCCGATTGAACCTGTTGAGAGTATGCCTTTATGTTACATCAAAACA GATATCACAGAAACCACTGGTAGAGAGAACACTAATGAAGCCTCCTCTGAAGGGAACTCTTCTGATGAT TCTGAAGATGAGCGAGTTAAGCGTCTTGCAAAGCTTCAGGAGCAGCTTAAAGCTGTACATCAACAGCTC CAGGTTTTGTCCCAAGTACCTTTCCGTAAGCTAAATAAAAAGAAAGAGAAGTCTAAAAAGGAAAAGAAA AAAGAAAAGGTTAATAACAGCAATGAAAATCCAAGAAAAATGTGTGAGCAAATGAGGCTAAAGGAAAAG TCCAAGAGAAATCAGCCAAAGAAAAGGAAACAACAGTTCATTGGTCTAAAATCTGAAGATGAAGATAAT GCTAAACCTATGAACTATGATGAGAAAAGGCAGTTAAGTCTGAATATAAACAAACTCCCTGGAGATAAA CTTGGGCGAGTAGTTCACATAATACAATCAAGAGAGCCTTCTCTGAGCAATTCCAATCCTGATGAGATA GAGATAGACTTTGAAACACTGAAAGCATCAACACTAAGAGAATTAGAAAAATATGTTTCGGCATGTCTA AGAAAGAGACCATTAAAACCTCCTGCTAAGAAAATAATGATGTCCAAAGAAGAACTTCACTCACAGAAA AAACAGGAATTGGAAAAGCGGTTACTGGATGTTAATAATCAGTTAAATTCTAGAAAACGTCAAACAAAA TCTGATAAAACGCAACCATCCAAAGCTGTTGAAAATGTTTCCCGACTGAGTGAGAGCAGCAGCAGCAGC AGCAGCTCATCAGAGTCTGAAAGTAGCAGCAGTGACTTAAGCTCTTCAGACAGCAGTGATTCTGAATCA GAAATGTTCCCTAAGTTTACAGAAGTAAAACCAAATGATTCTCCTTCTAAAGAGAATGTAAAGAAAATG AAGAATGAATGCATACTGCCTGAAGGAAGAACAGGCGTCACACAGATAGGATATTGTGTGCAAGACACA ACCTCTGCCAATACTACCCTTGTTCATCAGACCACACCTTCACATGTAATGCCACCAAATCACCACCAA TTAGCATTTAATTATCAAGAATTAGAACATTTACAGACTGTGAAAAACATTTCACCTTTACAAATTCTG CCTCCCTCAGGTGATTCTGAACAGCTCTCAAATGGCATAACTGTGATGCATCCATCTGGTGATAGTGAC ACAACGATGTTAGAATCTGAATGTCAAGCTCCTGTACAGAAGGATATAAAGATTAAGAATGCAGATTCA TGGAAAAGTTTAGGCAAACCAGTGAAACCATCAGGTGTAATGAAATCCTCAGATGAGCTCTTCAACCAA TTTAGAAAAGCAGCCATAGAAAAGGAAGTAAAAGCTCGGACACAGGAACTCATACGGAAGCATTTGGAA CAAAATACAAAGGAACTAAAAGCATCTCAAGAAAATCAGAGGGATCTTGGGAATGGATTGACTGTAGAA TCTTTTTCAAATAAAATACAAAACAAGTGCTCTGGAGAAGAGCAGAAAGAACATCAGCAGTCATCAGAA GCTCAAGATAAATCCAAACTCTGGCTTCTCAAAGACCGTGATTTAGCAAGGCAGAAAGAACAAGAGAGG AGGAGGAGAGAAGCAATGGTGGGTACCATTGATATGACCCTTCAAAGTGACATTATGACAATGTTTGAA AACAACTTTGATTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001242807
Insert Size	2706 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001242807.1
RefSeq Size	3029 bp
RefSeq ORF	2706 bp
Locus ID	676
UniProt ID	Q58F21
Protein Families	Protein Kinase, Transcription Factors
MW	102.4 kDa
Gene Summary	BRDT is similar to the RING3 protein family. It possesses 2 bromodomain motifs and a PEST sequence (a cluster of proline, glutamic acid, serine, and threonine residues), characteristic of proteins that undergo rapid intracellular degradation. The bromodomain is found in proteins that regulate transcription. Several transcript variants encoding multiple isoforms have been found for this gene. [provided by RefSeq, Jun 2011] Transcript Variant: This variant (5) differs in the 5' UTR, lacks an alternate in-frame exon, and uses an alternate in-frame splice junction at the 3' end of an exon compared to variant 4. The resulting isoform (c) lacks two internal segments compared to isoform a. Variants 5 and 6 both encode the same protein.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.