BRF1 (NM_001242786) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	BRF; BRF-1; CFDS; GTF3B; hBRF; HEL-S-76p; TAF3B2; TAF3C; TAFIII90; TF3B90; TFIIIB90
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331856 representing NM_001242786. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGCCGTGAGCAGGCACCTGACCCGCGGCCGGAAGATGGCCCACGTGATTGCTGCCTGCCTCTACCTG GTCTGCCGTACGGAGGGCACGCCGCACATGCTCCTGGACCTCAGCGACCTGCTCCAGGTGAATGTGTAC GTGCTTGGAAAGACGTTTCTTCTCTTGGCAAGAGAGCTCTGCATCAATGCGCCGGCCATAGACCCGTGC CTGTATATTCCACGCTTTGCGCACCTGCTGGAATTCGGGGAGAAGAACCACGAGGTGTCCATGACTGCC CTGAGGCTCCTACAGAGGATGAAGCGGGACTGGATGCACACAGGCCGGCGCCCCTCGGGCCTCTGCGGA GCAGCGCTCCTGGTTGCAGCCAGAATGCATGACTTCAGGAGGACTGTGAAGGAGGTCATCAGTGTGGTC AAAGTGTGTGAGTCCACGCTGCGGAAGAGGCTCACGGAATTTGAAGACACCCCCACCAGTCAGTTGACC ATTGATGAGTTCATGAAGATCGACCTGGAGGAGGAGTGCGACCCCCCCTCGTACACAGCTGGGCAGAGG AAGCTGCGGATGAAGCAGCTTGAACAAGTCCTGTCAAAAAAACTGGAGGAGGTTGAAGGTGAAATATCC AGTTACCAGGATGCAATTGAGATTGAACTAGAAAACAGCCGGCCAAAGGCCAAGGGGGGCCTGGCCAGC CTGGCAAAAGATGGCTCCACCGAGGACACCGCGTCCAGCTTGTGTGGCGAGGAGGACACAGAGGACGAG GAGCTGGAAGCCGCGGCCAGCCACCTGAACAAAGACTTATACCGGGAGCTCCTTGGTGGTGCCCCCGGC AGCTCGGAAGCAGCAGGAAGCCCCGAGTGGGGCGGCAGACCTCCGGCCCTGGGGTCCCTGCTGGACCCC CTCCCCACTGCAGCCAGCCTGGGCATCTCAGACTCCATCCGCGAATGCATCTCCTCTCAGAGCAGCGAC CCCAAAGATGCTTCAGGAGACGGTGAGCTGGACCTCAGTGGCATTGATGACCTGGAGATTGACAGGCGC GACCTTAGCATGCCCCGGTGTGCGAAGGCTAAAAGCCAGCCCCACTTCCCTGTGCTCGCCCAGTACATC CTGAATGAGTCGGAAGCCCGCGTGAAGGCCGAGCTGTGGATGAGGGAGAACGCCGAGTACCTGCGGGAA CAGAGGGAAAAAGAAGCAAGAATAGCGAAAGAGAAGGAGCTCGGCATCTACAAGGAACACAAGCCCAAG AAGTCTTGCAAGCGACGGGAGCCAATTCAGGCCAGTACCGCCAGGGAGGCCATCGAGAAGATGCTGGAG CAGAAGAAGATCTCCAGCAAGATCAATTATAGCGTGCTCCGGGGCCTCAGCAGCGCCGGCGGGGGCAGT CCGCACAGGGAGGATGCACAGCCCGAGCATAGCGCCAGTGCCAGGAAGCTGTCACGAAGGAGGACGCCG GCCAGCAGAAGTGGGGCTGACCCTGTGACCAGTGTGGGGAAAAGGTTGAGGCCTCTGGTGTCTACGCAG CCAGCAAAGAAGGTGGCCACGGGAGAGGCTTTGCTCCCAAGCTCTCCCACCCTCGGAGCTGAGCCTGCC AGGCCCCAGGCGGTGCTGGTGGAGAGCGGGCCCGTGTCATACCACGCCGACGAGGAGGCTGACGAGGAG GAGCCTGACGAGGAGGACGGGGAGCCCTGCGTCAGTGCCCTGCAGATGATGGGCAGCAACGACTATGGC TGTGATGGCGATGAGGACGACGGCTACTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001242786
Insert Size	1755 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001242786.1
RefSeq Size	3452 bp
RefSeq ORF	1755 bp
Locus ID	2972
UniProt ID	Q92994
Protein Families	Transcription Factors
MW	64.3 kDa
Gene Summary	This gene encodes one of the three subunits of the RNA polymerase III transcription factor complex. This complex plays a central role in transcription initiation by RNA polymerase III on genes encoding tRNA, 5S rRNA, and other small structural RNAs. The gene product belongs to the TF2B family. Several alternatively spliced variants encoding different isoforms, that function at different promoters transcribed by RNA polymerase III, have been identified. [provided by RefSeq, Jun 2011] Transcript Variant: This variant (4) differs in the 5' UTR and coding sequence and uses an alternate in-frame splice junction at the 5' end of an exon compared to variant (1). The resulting isoform (4) is shorter at the N-terminus and contains an insert compared to isoform 1.

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