DLGAP1 (NM_001242764) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DAP-1; DAP-1-ALPHA; DAP-1-BETA; DAP1; DLGAP1A; DLGAP1B; GKAP; SAPAP1
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331850 representing NM_001242764. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGATCGACCTTTTTAAGGCTGAGTGGGTTTCTTCAGTTTGTGTGCAAGTTTCAAGAAATGGAAGGACT GACCAGGTTCCACAAGATGAATGGACAGGGTACACCCCACGAGGTAAAGATGATGAAATTCCATGCCGA AGAATGCGGAGTGGCAGTTATATCAAGGCCATGGGGGATGAAGACAGTGGAGACTCAGACACGAGTCCT AAGCCTTCTCCAAAAGTTGCTGCGCGGAGAGAAAGCTATCTCAAGGCTACTCAGCCATCCCTTACAGAA CTCACCACACTCAAAATCTCCAATGAACACTCACCCAAACTCCAGATCCGGAGTCATAGTTACCTGAGG GCAGTGAGTGAAGTCTCCATCAACCGGAGCCTGGACAGCCTGGACCCTGCAGGCTTGCTCACATCACCA AAGTTCCGCTCCAGGAATGAGAGCTACATGCGAGCCATGAGCACCATCAGCCAGGTGAGCGAGATGGAA GTGAACGGGCAGTTCGAGTCCGTGTGCGAGTCCGTGTTCAGCGAGCTGGAGTCGCAGGCCGTGGAAGCG CTGGACCTGCCCATGCCCGGCTGCTTCCGCATGCGGAGCCACAGCTATGTGCGGGCCATTGAGAAAGGC TGCTCCCAGGACGACGAGTGCGTGTCCCTGAGGTCGTCCTCGCCGCCGCGCACCACCACCACCGTTAGG ACCATCCAGAGCAGCACGGTGTCATCTTGCATTACAACATATAAGAAGACACCACCTCCAGTCCCACCC AGAACTACCACGAAACCTTTCATTTCTATCACAGCCCAGAGTAGCACAGAGTCAGCCCAGGATGCCTAC ATGGACGGACAGGGCCAGCGAGGAGATATTATCAGCCAGTCTGGACTCAGCAACTCCACCGAGAGCCTG GACAGTATGAAGGCTCTGACAGCCGCCATCGAAGCTGCAAACGCCCAGATCCATGGCCCTGCCAGTCAA CACATGGGCAATAACACTGCCACCGTCACCACCACGACTACCATAGCCACCGTCACCACGGAGGACAGG AAGAAGGACCACTTTAAGAAAAATCGATGCCTGTCTATCGGGATACAGGTGGATGATGCTGAAGAACCT GACAAAACAGGGGAGAATAAAGCACCCAGTAAGTTCCAGTCCGTGGGAGTGCAAGTAGAAGAAGAGAAG TGCTTCCGCAGGTTCACTCGATCCAACAGTGTGACGACAGCAGTACAGGCCGACCTGGACTTCCATGAT AATCTGGAAAATTCTCTGGAATCTATAGAGGACAATTCGTGTCCTGGCCCCATGGCCAGACAGTTCTCC CGCGATGCCAGCACCTCCACAGTCAGCATTCAGGGCTCAGGAAACCATTACCATGCCTGTGCCGCCGAT GATGACTTTGACACGGATTTTGACCCCTCTATTCTGCCTCCTCCGGACCCCTGGATTGACTCTATCACT GAAGACCCTCTGGAGGCCGTGCAAAGGTCAGTGTGCCACCGGGATGGCCACTGGTTCCTGAAGCTTCTC CAGGCAGAGCGAGACCGCATGGAGGGGTGGTGTCAACAGATGGAGCGGGAAGAACGGGAAAACAACCTG CCCGAAGACATTCTAGGAAAAATCCGAACCGCAGTGGGCAGTGCCCAACTTCTCATGGCCCAGAAATTC TACCAGTTCAGAGAACTGTGTGAAGAAAACCTGAATCCTAATGCTCATCCAAGACCCACCTCCCAGGAT TTGGCGGGGTTTTGGGACATGCTGCAGTTGTCCATAGAAAATATTAGTATGAAATTTGATGAACTTCAT CAGTTAAAGGCCAATAATTGGAAACAGATGGATCCTCTTGACAAGAAGGAGAGAAGGGCCCCTCCTCCA GTGCCAAAGAAGCCGGCGAAGGGCCCCGCGCCGCTGATCCGGGAGCGCTCGCTGGAGAGCTCGCAGCGC CAGGAGGCCCGCAAGCGCCTGATGGCCGCCAAGCGCGCCGCGTCCGTCCGCCAGAACTCGGCCACCGAG AGCGCCGAGAGCATCGAGATCTACATCCCCGAGGCGCAGACCCGGCTCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001242764
Insert Size	2052 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001242764.1
RefSeq Size	5244 bp
RefSeq ORF	2052 bp
Locus ID	9229
UniProt ID	O14490
MW	76.2 kDa
Gene Summary	Part of the postsynaptic scaffold in neuronal cells.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (6) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (5) has a distinct N-terminus and is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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