DLGAP1 (NM_001242763) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DAP-1; DAP-1-ALPHA; DAP-1-BETA; DAP1; DLGAP1A; DLGAP1B; GKAP; SAPAP1
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331849 representing NM_001242763. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGATTTGAAGACATTAAAACTGTTTAATTCTCAGTTGAGATGTGGTTGGCTCTTATACATTTGGAAT AAAGCATTTATGGCACATCTCAGGGTTCCACAAGATGAATGGACAGGGTACACCCCACGAGGTAAAGAT GATGAAATTCCATGCCGAAGAATGCGGAGTGGCAGTTATATCAAGGCCATGGGGGATGAAGACAGTGGA GACTCAGACACGAGTCCTAAGCCTTCTCCAAAAGTTGCTGCGCGGAGAGAAAGCTATCTCAAGGCTACT CAGCCATCCCTTACAGAACTCACCACACTCAAAATCTCCAATGAACACTCACCCAAACTCCAGATCCGG AGTCATAGTTACCTGAGGGCAGTGAGTGAAGTCTCCATCAACCGGAGCCTGGACAGCCTGGACCCTGCA GGCTTGCTCACATCACCAAAGTTCCGCTCCAGGAATGAGAGCTACATGCGAGCCATGAGCACCATCAGC CAGGTGAGCGAGATGGAAGTGAACGGGCAGTTCGAGTCCGTGTGCGAGTCCGTGTTCAGCGAGCTGGAG TCGCAGGCCGTGGAAGCGCTGGACCTGCCCATGCCCGGCTGCTTCCGCATGCGGAGCCACAGCTATGTG CGGGCCATTGAGAAAGGCTGCTCCCAGGACGACGAGTGCGTGTCCCTGAGGTCGTCCTCGCCGCCGCGC ACCACCACCACCGTTAGGACCATCCAGAGCAGCACGGGTGTCATAAAACTGAGTTCTGCAGTTGAAGTG TCATCTTGCATTACAACATATAAGAAGACACCACCTCCAGTCCCACCCAGAACTACCACGAAACCTTTC ATTTCTATCACAGCCCAGAGTAGCACAGAGTCAGCCCAGGATGCCTACATGGACGGACAGGGCCAGCGA GGAGATATTATCAGCCAGTCTGGACTCAGCAACTCCACCGAGAGCCTGGACAGTATGAAGGCTCTGACA GCCGCCATCGAAGCTGCAAACGCCCAGATCCATGGCCCTGCCAGTCAACACATGGGCAATAACACTGCC ACCGTCACCACCACGACTACCATAGCCACCGTCACCACGGAGGACAGGAAGAAGGACCACTTTAAGAAA AATCGATGCCTGTCTATCGGGATACAGGTGGATGATGCTGAAGAACCTGACAAAACAGGGGAGAATAAA GCACCCAGTAAGTTCCAGTCCGTGGGAGTGCAAGTAGAAGAAGAGAAGTGCTTCCGCAGGTTCACTCGA TCCAACAGTGTGACGACAGCAGTACAGGCCGACCTGGACTTCCATGATAATCTGGAAAATTCTCTGGAA TCTATAGAGGACAATTCGTGTCCTGGCCCCATGGCCAGACAGTTCTCCCGCGATGCCAGCACCTCCACA GTCAGCATTCAGGGCTCAGGAAACCATTACCATGCCTGTGCCGCCGATGATGACTTTGACACGGATTTT GACCCCTCTATTCTGCCTCCTCCGGACCCCTGGATTGACTCTATCACTGAAGACCCTCTGGAGGCCGTG CAAAGGTCAGTGTGCCACCGGGATGGCCACTGGTTCCTGAAGCTTCTCCAGGCAGAGCGAGACCGCATG GAGGGGTGGTGTCAACAGATGGAGCGGGAAGAACGGGAAAACAACCTGCCCGAAGACATTCTAGGAAAA ATCCGAACCGCAGTGGGCAGTGCCCAACTTCTCATGGCCCAGAAATTCTACCAGTTCAGAGAACTGTGT GAAGAAAACCTGAATCCTAATGCTCATCCAAGACCCACCTCCCAGGATTTGGCGGGGTTTTGGGACATG CTGCAGTTGTCCATAGAAAATATTAGTATGAAATTTGATGAACTTCATCAGTTAAAGGCCAATAATTGG AAACAGATGGATCCTCTTGACAAGAAGGAGAGAAGGGCCCCTCCTCCAGTGCCAAAGAAGCCGGCGAAG GGCCCCGCGCCGCTGATCCGGGAGCGCTCGCTGGAGAGCTCGCAGCGCCAGGAGGCCCGCAAGCGCCTG ATGGCCGCCAAGCGCGCCGCGTCCGTCCGCCAGAACTCGGCCACCGAGAGCGCCGAGAGCATCGAGATC TACATCCCCGAGGCGCAGACCCGGCTCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001242763
Insert Size	2100 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001242763.1
RefSeq Size	5328 bp
RefSeq ORF	2100 bp
Locus ID	9229
UniProt ID	O14490
MW	78.1 kDa
Gene Summary	Part of the postsynaptic scaffold in neuronal cells.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (5) lacks a portion of the 5' UTR and 5' coding region, initiates translation at an alternate start codon, and has multiple coding region differences, compared to variant 1. The encoded isoform (5) has a distinct N-terminus and is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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