CLASP1 (NM_001207051) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	MAST1
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331746 representing NM_001207051. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGAGCCTCGCATGGAGTCCTGCCTGGCGCAGGTGTTGCAGAAGGATGTGGGGAAACGATTGCAGGTT GGCCAAGAACTGATAGACTATTTCTCAGACAAACAGAAGTCTGCTGACCTTGAGCATGACCAGACCATG TTAGATAAACTTGTGGATGGACTTGCTACCTCTTGGGTGAACTCTAGCAATTACAAGGTGGTTCTGCTG GGCATGGACATCCTGTCCGCCCTGGTGACCCGGCTGCAGGATCGGTTCAAGGCGCAGATCGGCACAGTG CTGCCAAGTCTAATAGACAGACTAGGAGATGCTAAAGACTCTGTGAGGGAGCAGGACCAAACTCTGCTG CTAAAGATCATGGATCAAGCTGCTAATCCCCAGTACGTATGGGACAGAATGCTTGGAGGCTTCAAACAC AAGAATTTCCGTACTCGAGAAGGCATCTGTCTCTGCCTTATAGCAACACTCAATGCCTCTGGAGCACAG ACTTTAACACTAAGCAAGATTGTGCCACATATATGCAACTTACTTGGAGATCCAAACAGCCAGGTTCGA GATGCAGCAATAAACAGCTTAGTGGAAATTTACAGACATGTAGGAGAACGTGTGAGGGCAGATCTCAGT AAAAAAGGATTGCCACAGTCCCGGTTGAATGTAATTTTTACAAAATTTGATGAAGTCCAGAAATCTGGA AACATGATACAATCTGCAAATGATAAAAATTTCGACGATGAAGATTCTGTGGATGGTAACAGACCTTCC TCTGCTAGTTCTACATCATCCAAGGCTCCACCAAGTTCTCGGAGAAACGTTGGAATGGGAACCACCCGC CGGCTTGGTTCATCCACCCTTGGATCCAAGTCTTCAGCTGCAAAAGAAGGAGCTGGTGCTGTTGATGAA GAGGATTTTATTAAAGCATTTGATGATGTACCTGTAGTACAGATTTATTCCAGCCGAGACCTTGAGGAA TCCATAAACAAAATTAGGGAAATATTATCTGATGACAAGCATGATTGGGAGCAGAGAGTAAATGCTCTA AAAAAGATTAGATCTTTACTTTTGGCTGGTGCTGCTGAGTATGATAACTTCTTTCAACATTTGCGTCTT TTGGATGGAGCCTTTAAACTCTCTGCTAAGGACCTGCGGTCTCAGGTAGTGCGGGAGGCTTGTATCACG TTGGGGCATCTGTCATCAGTTCTGGGGAATAAGTTTGACCATGGAGCTGAAGCCATTATGCCAACTATC TTTAATTTAATTCCAAACAGTGCCAAAATTATGGCCACATCTGGTGTTGTAGCTGTTAGGTTAATTATT CGGCACACACACATCCCTAGGTTAATACCTGTCATAACAAGCAACTGTACCTCTAAGTCTGTCGCAGTT AGAAGGCGCTGTTTTGAATTTTTAGATTTGCTTTTACAAGAATGGCAGACACATTCACTAGAACGACAC ATATCAGTATTAGCTGAAACAATAAAGAAGGGAATACATGATGCTGATTCCGAAGCAAGAATAGAAGCC AGAAAATGTTACTGGGGTTTCCACAGTCACTTCAGCAGAGAAGCAGAGCACTTGTACCACACCTTGGAG TCCTCCTACCAGAAAGCCCTGCAGTCCCACCTGAAGAACTCAGACAGCATAGTGTCTCTGCCTCAGTCA GACCGCTCATCTTCCAGCTCTCAAGAGAGTCTAAATCGTCCGCTGTCTGCCAAAAGAAGTCCTACTGGA AGTACCACATCTAGAGCTTCTACAGTTAGTACCAAATCTGTGTCAACGACTGGGTCCCTCCAGCGATCT CGAAGTGATATTGATGTGAACGCAGCAGCCAGTGCCAAATCCAAAGTCTCCTCATCTTCGGGCACGACG CCTTTCAGCTCTGCAGCAGCTTTGCCTCCAGGGTCATACGCATCCTTAGAGTCCAGACACATGAGGGAA GACATGGAGTACATAGGCCTGGACTCAGGTCGGATCCGCACAAGACGGCAAAGCTCTGGGAGTGCCACC AACGTCGCCTCTACACCTGATAACCGGGGCCGCAGTCGCGCTAAAGTGGTTTCACAGTCCCAGCCTGGC AGCCGGTCAAGTTCCCCAGGAAAATTGTTGGGAAGTGGTTATGGTGGACTTACTGGGGGCTCCTCACGA GGCCCACCTGTGACACCGTCTTCAGAAAAGCGAAGCAAGATTCCCAGGAGCCAGGGATGTAGCCGGGAA ACAAGTCCAAACCGAATAGGATTAGATCGGTTTGGGCTTGGCCAGCCAGGAAGAATACCTGGTTCTGTG AATGCCATGAGAGTTCTGAGCACAAGTACAGATCTTGAAGCTGCTGTTGCTGATGCTTTGCTGTTAGGA GACTCCAGGAGCAAGAAGCCTGTGAGGAGGAGATATGAGCCGTATGGGATGTATTCTGACGATGATGCC AACAGTGATGCCTCAAGTGTTTGCTCTGAGCGCTCATATGGCTCCAGGAATGGTGGCATTCCCCATTAT CTGCGGCAGACTGAGGATGTAGCAGAAGTTCTCAACCACTGTGCTAGTTCAAACTGGTCAGAAAGGAAA GAAGGGCTTCTGGGCCTGCAGAACTTACTGAAGAGCCAAAGAACACTGAGTCGAGTTGAACTGAAAAGG TTGTGTGAGATCTTCACTCGGATGTTTGCTGACCCTCATAGCAAGAGAGTTTTCAGTATGTTTTTGGAG ACTCTTGTGGATTTTATAATAATTCATAAGGATGATTTACAAGACTGGCTTTTTGTTCTTCTCACACAA TTACTTAAGAAAATGGGAGCAGATTTACTTGGATCTGTGCAAGCAAAAGTTCAAAAGGCTCTAGATGTC ACAAGGGACTCCTTTCCATTTGATCAACAATTTAACATTTTGATGAGATTTATTGTGGATCAAACTCAA ACTCCAAACCTCAAGGTCAAAGTTGCAATCCTGAAATACATTGAGTCTCTGGCCAGACAGATGGATCCA ACAGATTTTGTAAACTCTAGTGAGACAAGGCTTGCTGTTTCTAGAATCATAACCTGGACAACAGAACCA AAGAGTTCAGACGTGAGAAAGGCAGCACAGATTGTGCTAATCTCTCTGTTTGAATTGAATACTCCTGAA TTTACCATGTTACTTGGTGCCTTGCCAAAAACATTCCAGGATGGTGCCACCAAACTCCTGCACAACCAC CTCAAGAATTCCAGTAACACCAGTGTGGGCTCTCCAAGCAATACGATTGGCCGGACGCCCTCCCGACAC ACCAGCAGCAGGACCAGCCCCCTGACCTCACCCACCAACTGTTCCCATGGGGGTCTGTCTCCAAGCATG CTGGACTATGATACAGAGAACCTGAACTCTGAAGAAATCTATAGTTCTCTACGTGGAGTTACAGAAGCC ATTGAAAAGTTTAGTTTTCGAAGCCAAGAAGATCTGAATGAGCCAATTAAACGAGATGGCAAAAAGGAG TGTGATATTGTGTCCCGCGATGGGGGCGCTGCCTCCCCTGCCACTGAGGGCCGGGGGGGTAGTGAAGTA GAAGGAGGCCGGACAGCTCTGGATAACAAGACCTCACTACTCAACACCCAGCCTCCGCGCGCCTTCCCG GGGCCGCGGGCGCGAGACTACAACCCGTACCCCTACTCAGATGCCATCAACACCTACGACAAGACCGCC CTGAAAGAGGCTGTGTTCGATGACGACATGGAGCAGCTTCGAGACGTGCCCATCGACCATTCTGACCTG GTGGCTGACCTTCTGAAAGAGCTGTCCAACCACAATGAGCGAGTGGAGGAACGGAAGGGAGCCCTGCTG GAGCTGCTCAAGATCACGCGGGAAGACAGCCTTGGTGTCTGGGAGGAGCACTTCAAGACCATTCTGCTC CTGCTGCTGGAGACCCTTGGAGACAAAGACCATTCAATTCGAGCACTGGCGTTAAGAGTTTTGAGGGAA ATTCTGAGAAATCAACCAGCAAGATTTAAAAACTACGCCGAGCTGACGATTATGAAGACTCTGGAAGCC CACAAAGACTCCCATAAGGAGGTGGTGAGAGCGGCTGAGGAGGCTGCGTCCACACTGGCCAGTTCCATC CACCCGGAGCAGTGCATCAAGGTGCTCTGCCCCATCATCCAGACGGCCGACTACCCCATCAACCTTGCT GCCATCAAGATGCAGACCAAAGTCGTCGAGAGGATCGCAAAGGAGTCATTGCTGCAGCTCCTTGTCGAC ATCATCCCAGGCTTGCTGCAGGGTTATGACAACACCGAAAGTAGTGTGCGTAAGGCCAGCGTGTTTTGC TTAGTGGCAATTTATTCCGTAATCGGAGAAGACCTGAAACCTCACCTTGCACAGCTCACAGGGAGCAAG ATGAAGCTACTAAACTTATACATAAAGAGGGCCCAGACCACCAACAGCAACAGCAGCTCCTCCTCCGAT GTCTCCACGCACAGCTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001207051
Insert Size	4434 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001207051.1
RefSeq Size	7935 bp
RefSeq ORF	4434 bp
Locus ID	23332
UniProt ID	Q7Z460
MW	163.1 kDa
Gene Summary	CLASPs, such as CLASP1, are nonmotor microtubule-associated proteins that interact with CLIPs (e.g., CLIP170; MIM 179838). CLASP1 is involved in the regulation of microtubule dynamics at the kinetochore and throughout the spindle (Maiato et al., 2003 [PubMed 12837247]).[supplied by OMIM, Mar 2008] Transcript Variant: This variant (4) has several differences in the coding region, compared to variant 1. It encodes isoform 4, which is shorter than isoform 1.

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