ZNRF3 (NM_001206998) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	BK747E2.3; RNF203
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331727 representing NM_001206998. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGAGGCCGCGCTCGGGCGGGCGCCCAGGGGCCACGGGCCGCCGCCGCCGCCGCCTGCGCCGCCGCCCC CGCGGCCTCCGGTGCAGCCGCCTGCCGCCGCCGCCGCCGCTGCCGCTGCTGCTCGGGCTGCTGCTGGCG GCCGCGGGGCCCGGCGCGGCGCGGGCCAAGGAGACGGCGTTCGTGGAGGTGGTGCTGTTCGAGTCGAGC CCAAGCGGCGATTACACCACCTACACCACCGGCCTCACGGGCCGCTTCTCGCGGGCCGGGGCCACGCTC AGCGCCGAGGGCGAGATCGTGCAGATGCACCCACTGGGCCTATGTAATAACAATGACGAAGAGGACTTG TATGAATATGGCTGGGTAGGAGTGGTGAAGCTGGAACAGCCAGAATTGGACCCGAAACCATGCCTCACT GTCCTAGGCAAGGCCAAGCGAGCAGTACAGCGGGGAGCTACTGCAGTCATCTTTGATGTGTCTGAAAAC CCAGAAGCTATTGATCAGCTGAACCAGGGCTCTGAAGACCCGCTCAAGAGGCCGGTGGTGTATGTGAAG GGTGCAGATGCCATTAAGCTGATGAACATCGTCAACAAGCAGAAAGTGGCTCGAGCAAGGATCCAGCAC CGCCCTCCTCGACAACCCACTGAATACTTTGACATGGGGATTTTCCTGGCTTTCTTCGTCGTGGTCTCC TTGGTCTGCCTCATCCTCCTTGTCAAAATCAAGCTGAAGCAGCGACGCAGTCAGAATTCCATGAACAGG CTGGCTGTGCAGGCTCTAGAGAAGATGGAAACCAGAAAGTTCAACTCCAAGAGCAAGGGGCGCCGGGAG GGGAGCTGTGGGGCCCTGGACACACTCAGCAGCAGCTCCACGTCCGACTGTGCCATCTGTCTGGAGAAG TACATTGATGGAGAGGAGCTGCGGGTCATCCCCTGTACTCACCGGTTTCACAGGAAGTGCGTGGACCCC TGGCTGCTGCAGCACCACACCTGCCCCCACTGTCGGCACAACATCATAGAACAAAAGGGAAACCCAAGC GCGGTGTGTGTGGAGACCAGCAACCTCTCACGTGGTCGGCAGCAGAGGGTGACCCTGCCGGTGCATTAC CCCGGCCGCGTGCACAGGACCAACGCCATCCCAGCCTACCCTACGAGGACAAGCATGGACTCCCACGGC AACCCCGTCACCTTGCTGACCATGGACCGGCACGGGGAGCAGAGCCTCTATTCCCCGCAGACCCCCGCC TACATCCGCAGCTACCCACCCCTCCACCTGGACCACAGCCTGGCCGCTCACCGCTGCGGCCTGGAGCAC CGGGCCTACTCCCCAGCCCACCCCTTCCGCAGGCCCAAGTTGAGTGGCCGCAGCTTCTCCAAGGCAGCT TGCTTCTCCCAGTATGAGACCATGTACCAGCACTACTACTTCCAGGGCCTCAGCTACCCGGAGCAGGAG GGGCAGTCCCCACCTAGCCTCGCACCCCGGGGCCCGGCCCGTGCCTTTCCTCCGAGCGGCAGTGGCAGC CTGCTCTTCCCCACCGTGGTGCACGTGGCCCCGCCCTCCCACCTGGAGAGCGGCAGCACGTCCAGCTTC AGCTGCTATCACGGCCACCGCTCGGTGTGCAGTGGCTACCTGGCCGACTGCCCAGGCAGCGACAGCAGC AGCAGCAGCAGCTCCGGCCAGTGCCACTGTTCCTCCAGTGACTCTGTGGTAGACTGCACTGAGGTCAGC AACCAGGGCGTGTACGGGAGCTGCTCCACCTTCCGCAGCTCCCTCAGCAGCGACTATGACCCCTTCATC TACCGCAGCCGGAGCCCCTGTCGTGCCAGTGAGGCGGGGGGCTCGGGCAGCTCGGGCCGGGGACCTGCC CTGTGCTTCGAGGGCTCCCCGCCTCCCGAGGAGCTCCCGGCGGTGCACAGTCATGGTGCTGGGCGGGGC GAGCCTTGGCCGGGCCCTGCCTCTCCCTCGGGGGATCAGGTGTCCACCTGCAGCCTGGAGATGAACTAC AGCAGCAACTCCTCCCTGGAGCACAGGGGGCCCAATAGCTCTACCTCAGAAGTGGGGCTCGAGGCTTCT CCTGGGGCCGCCCCTGACCTCAGGAGGACCTGGAAGGGGGGCCACGAGTTGCCGTCGTGTGCCTGCTGC TGCGAGCCCCAGCCCTCCCCAGCCGGGCCTAGCGCCGGAGCAGCTGGCAGCAGCACCTTGTTCCTGGGG CCCCACCTCTACGAGGGCTCTGGCCCGGCGGGTGGGGAGCCCCAGTCAGGAAGCTCCCAGGGCTTGTAC GGCCTTCACCCCGACCATTTGCCCAGGACAGATGGGGTGAAATACGAGGGTCTGCCCTGCTGCTTCTAT GAAGAGAAGCAGGTGGCCCGCGGGGGCGGAGGGGGCAGCGGCTGCTACACTGAGGACTACTCGGTGAGT GTGCAGTACACGCTCACCGAGGAACCACCGCCCGGCTGCTACCCCGGGGCCCGGGACCTGAGCCAGCGC ATCCCCATCATTCCAGAGGATGTGGACTGTGATCTGGGCCTGCCCTCGGACTGCCAAGGGACCCACAGC CTCGGCTCCTGGGGTGGGACGCGAGGCCCGGATACCCCACGGCCCCACAGGGGCCTGGGAGCAACCCGG GAAGAGGAGCGGGCTCTGTGCTGCCAGGCTAGGGCCCTACTGCGGCCTGGCTGCCCTCCGGAGGAGGCG GGTGCTGTCAGGGCCAACTTCCCTAGTGCCCTCCAGGACACTCAGGAGTCCAGCACCACTGCCACTGAG GCTGCAGGACCGAGATCTCACTCAGCAGACAGCAGCAGCCCGGGAGCCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001206998
Insert Size	2811 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001206998.1
RefSeq Size	6696 bp
RefSeq ORF	2811 bp
Locus ID	84133
UniProt ID	Q9ULT6
Protein Families	Druggable Genome, Transmembrane
MW	100.6 kDa
Gene Summary	E3 ubiquitin-protein ligase that acts as a negative regulator of the Wnt signaling pathway by mediating the ubiquitination and subsequent degradation of Wnt receptor complex components Frizzled and LRP6. Acts on both canonical and non-canonical Wnt signaling pathway. Acts as a tumor suppressor in the intestinal stem cell zone by inhibiting the Wnt signaling pathway, thereby resticting the size of the intestinal stem cell zone (PubMed:22575959). Along with RSPO2 and RNF43, constitutes a master switch that governs limb specification (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

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