PLA1A (NM_001206961) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	PS-PLA1; PSPLA1
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331722 representing NM_001206961. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGTGGGACAGCTCTTCGGAGGCCAGCTGGGACAGATCACAGGCCTGGACCCCGCTGGACCTGAGTAC ACCAGGGCCAGTGTGGAAGAGCGCTTGGATGCTGGAGATGCCCTCTTCGTGGAAGCCATCCACACAGAC ACCGACAATTTGGGTATTCGGATTCCCGTTGGACATGTGGACTACTTCGTCAACGGAGGCCAAGACCAA CCTGGCTGCCCCACCTTCTTTTACGCAGGTTATAGTTATCTGATCTGTGATCACATGAGGGCTGTGCAC CTCTACATCAGCGCCCTGGAGAATTCCTGTCCACTGATGGCCTTTCCCTGTGCCAGCTACAAGGCCTTC CTTGCTGGACGCTGTCTGGATTGCTTTAACCCTTTTCTGCTTTCCTGCCCAAGGATAGGACTGGTGGAA CAAGGTGGTGTCAAGATAGAGCCGCTCCCCAAGGAAGTGAAAGTCTACCTCCTGACTACTTCCAGTGCT CCGTACTGCATGCATCACAGCCTCGTGGAGTTTCACTTGAAGGAACTGAGAAACAAGGACACCAACATC GAGGTTACCTTCCTTAGCAGTAACATCACCTCTTCATCTAAGATCACCATACCTAAGCAGCAACGCTAT GGGAAAGGAATCATAGCCCATGCCACCCCACAATGCCAGATAAACCAAGTGAAATTCAAGTTTCAGTCT TCCAACCGAGTTTGGAAAAAAGACCGGACTACCATTATTGGGAAGTTCTGCACTGCCCTTTTGCCTGTC AATGACAGAGAAAAGATGGTCTGCTTACCTGAACCAGTGAACTTACAAGCAAGTGTGACTGTTTCCTGT GACCTGAAGATAGCCTGTGTGTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001206961
Insert Size	852 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001206961.1
RefSeq Size	1647 bp
RefSeq ORF	852 bp
Locus ID	51365
UniProt ID	Q53H76
Protein Families	Druggable Genome, Secreted Protein
MW	31.3 kDa
Gene Summary	The protein encoded by this gene is a phospholipase that hydrolyzes fatty acids at the sn-1 position of phosphatidylserine and 1-acyl-2-lysophosphatidylserine. This secreted protein hydrolyzes phosphatidylserine in liposomes. Three transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2011] Transcript Variant: This variant (3) lacks an alternate coding exon compared to variant 1. The resulting isoform (3) uses a downstream AUG start site and is shorter at the N-terminus compared to isoform 1.

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