ALDH1A2 (NM_001206897) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	RALDH(II); RALDH2; RALDH2-T
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331699 representing NM_001206897. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGAAGAATCAGTGTGAGACTGTTTGGTTAAAATCCCCAATAAAACTGAAACTGATCTTTATAAACAAC GAGTGGCAGAACTCAGAGAGTGGGAGAGTGTTCCCTGTCTATAATCCAGCCACAGGAGAACAGGTGTGT GAAGTTCAAGAAGCAGACAAGGCAGATATAGACAAAGCAGTGCAGGCAGCCCGCCTGGCTTTCTCTCTT GGTTCAGTGTGGAGAAGGATGGATGCTTCAGAAAGGGGACGTCTGTTGGATAAGCTTGCAGACTTGGTG GAACGGGACAGGGCAGTTCTTGCAACCATGGAATCCCTAAATGGTGGCAAACCATTCCTGCAAGCTTTT TATGTGGATTTGCAGGGCGTCATCAAAACCTTTCGATATTACGCAGGCTGGGCTGATAAAATTCATGGG ATGACCATTCCTGTAGATGGAGACTATTTTACCTTTACAAGACATGAACCCATTGGAGTGTGTGGACAG ATCATCCCATGGAACTTCCCCCTGCTGATGTTTGCCTGGAAAATAGCTCCAGCTTTGTGCTGTGGCAAT ACAGTAGTTATTAAGCCAGCAGAGCAAACACCACTCAGTGCACTCTACATGGGAGCCCTCATCAAGGAG GCTGGCTTTCCTCCCGGGGTCATCAATATTTTGCCAGGATATGGGCCAACGGCTGGGGCAGCAATAGCT TCTCACATTGGCATAGACAAGATTGCATTCACAGGGTCTACTGAGGTTGGAAAGCTTATCCAAGAAGCA GCTGGAAGAAGTAATTTGAAGAGAGTAACTCTGGAACTTGGAGGCAAAAGTCCTAATATTATTTTTGCT GATGCTGACTTGGACTATGCTGTGGAGCAGGCCCACCAGGGTGTGTTCTTCAATCAAGGTCAGTGCTGC ACTGCAGGCTCTCGCATCTTCGTGGAGGAGTCCATCTATGAGGAGTTTGTGAGAAGAAGCGTGGAGCGG GCCAAGAGGCGCGTAGTGGGGAGTCCCTTTGACCCCACCACTGAGCAGGGTCCCCAGATTGATAAGAAA CAGTACAACAAGATCTTGGAACTCATCCAGAGTGGTGTGGCTGAGGGCGCCAAGCTGGAATGTGGAGGC AAAGGACTGGGCCGAAAGGGGTTTTTCATTGAGCCCACAGTGTTTTCCAACGTCACTGATGATATGCGG ATTGCCAAGGAGGAGATCTTTGGCCCTGTTCAGGAAATTTTGAGATTTAAGACGATGGATGAAGTTATC GAAAGAGCCAATAACTCAGACTTTGGACTCGTAGCAGCTGTCTTTACTAATGACATCAACAAGGCCCTC ACAGTGTCTTCTGCAATGCAAGCTGGGACTGTTTGGATCAATTGTTACAATGCCTTAAATGCCCAGAGC CCCTTTGGGGGATTCAAGATGTCTGGAAATGGGAGAGAAATGGGAGAATTTGGCTTGCGGGAGTACTCA GAAGTTAAGACGGTGACAGTAAAGATCCCCCAGAAGAACTCCTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001206897
Insert Size	1494 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001206897.1
RefSeq Size	3703 bp
RefSeq ORF	1494 bp
Locus ID	8854
UniProt ID	O94788
Protein Families	Druggable Genome
Protein Pathways	Metabolic pathways, Retinol metabolism
MW	54.7 kDa
Gene Summary	This protein belongs to the aldehyde dehydrogenase family of proteins. The product of this gene is an enzyme that catalyzes the synthesis of retinoic acid (RA) from retinaldehyde. Retinoic acid, the active derivative of vitamin A (retinol), is a hormonal signaling molecule that functions in developing and adult tissues. The studies of a similar mouse gene suggest that this enzyme and the cytochrome CYP26A1, concurrently establish local embryonic retinoic acid levels which facilitate posterior organ development and prevent spina bifida. Four transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, May 2011] Transcript Variant: This variant (4) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (4) has a shorter and distinct N-terminus compared to isoform 1.

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