IRAG1 (NM_001206881) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	IRAG; JAW1L; MRVI1
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331698 representing NM_001206881. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGCCCTGAACAGCCCTCAGCCTGGCCCCGTGGAGAGCGAGCTGGGGAAGCAGCTCTTGAAAACGGGC TGGGAGGGCAGCCCTCTGCCGAGAAGTCCAACCCAGGATGCGGCAGGAGTGGGTCCCCCAGCCTCCCAG GGGAGAGGCCCAGCTGGAGAGCCGATGGGGCCCGAGGCTGGCTCCAAAGCTGAGCTTCCACCCACTGTG TCCCGGCCCCCGCTGCTGCGAGGGCTCTCCTGGGACAGTGGCCCTGAAGAACCTGGCCCCCGGCTGCAG AAAGTGCTTGCCAAGCTGCCACTGGCAGAGGAAGAAAAGCGTTTTGCAGGCAAGGCCGGCGGCAAGCTG GCCAAGGCCCCTGGTCTCAAAGACTTTCAGATACAAGTGCAGCCCGTGCGGATGCAGAAACTGACCAAG CTCCGAGAGGAGCACATCCTGATGAGAAATCAGAACTTAGTGGGGCTCAAGCTTCCAGACCTTAGTGAA GCAGCTGAGCAGGAAAAAGGGCTTCCTTCTGAACTCTCCCCAGCTATTGAGGAAGAAGAGTCAAAGAGT GGCTTAGATGTCATGCCTAATATTTCTGATGTGCTGCTGCGCAAACTGCGGGTCCACAGGAGTCTCCCT GGAAGTGCCCCTCCACTCACTGAAAAGGAAGTTGAGAACGTGTTTGTGCAACTGTCCTTGGCCTTTAGA AATGACAGCTACACTCTGGAATCTAGAATTAACCAGGCTGAAAGGGAACGCAACCTGACAGAGGAGAAC ACTGAGAAAGAACTGGAAAACTTCAAAGCTTCCATTACGTCCTCAGCTTCACTCTGGCACCACTGTGAG CACCGGGAAACCTACCAGAAGTTGCTGGAGGACATCGCTGTCCTGCACCGCCTGGCTGCCCGCCTCTCC AGCCGAGCTGAGGTGGTAGGCGCCGTCCGCCAGGAAAAGCGCATGTCGAAAGCAACGGAAGTGATGATG CAGTATGTGGAGAATCTAAAGAGGACGTATGAGAAGGACCATGCGGAGCTCATGGAGTTTAAAAAGCTT GCAAATCAGAATTCAAGCCGCAGCTGTGGCCCCTCTGAAGATGGGGTCCCTCGCACGGCACGGTCCATG TCCCTCACGCTGGGAAAGAATATGCCTCGCCGGAGGGTCAGCGTTGCTGTGGTTCCTAAGTTTAATGCC CTGAATCTGCCTGGCCAAACTCCCAGCTCATCATCCATTCCCTCCTTACCAGCCTTGTCGGAATCACCC AATGGGAAAGGCAGCCTACCTGTCACTTCAGCACTGCCTGCACTTTTGGAAAATGGAAAGACAAATGGG GACCCAGATTGTGAAGCCTCTGCTCCTGCGCTGACCCTGAGCTGCCTGGAGGAGCTTAGTCAGGAGACC AAGGCCAGGATGGAGGAAGAAGCCTACAGCAAGGGATTCCAAGAAGGTCTAAAGAAGACCAAAGAACTT CAAGACCTGAAGGAGGAGGAGGAAGAACAGAAGAGTGAGAGTCCTGAGGAACCTGAAGAGGTAGAAGAA ACTGAGGAAGAGGAAAAGGGCCCAAGAAGCAGCAAACTTGAAGAATTGGTCCATTTCTTACAAGTCATG TATCCCAAACTGTGTCAGCACTGGCAAGTGATCTGGATGATGGCTGCAGTGATGCTGGTCTTGACTGTT GTGCTGGGGCTCTACAATTCCTATAACTCTTGTGCAGAGCAGGCTGATGGGCCCCTTGGAAGATCCACT TGCTCGGCAGCCCAGAGGGACTCCTGGTGGAGCTCAGGACTCCAGCATGAGCAGCCTACAGAGCAGTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001206881
Insert Size	1794 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001206881.1
RefSeq Size	6165 bp
RefSeq ORF	1794 bp
Locus ID	10335
UniProt ID	Q9Y6F6
Protein Families	Transmembrane
Protein Pathways	Vascular smooth muscle contraction
MW	65.9 kDa
Gene Summary	This gene is similar to a putative mouse tumor suppressor gene (Mrvi1) that is frequently disrupted by mouse AIDS-related virus (MRV). The encoded protein, which is found in the membrane of the endoplasmic reticulum, is similar to Jaw1, a lymphoid-restricted protein whose expression is down-regulated during lymphoid differentiation. This protein is a substrate of cGMP-dependent kinase-1 (PKG1) that can function as a regulator of IP3-induced calcium release. Studies in mouse suggest that MRV integration at Mrvi1 induces myeloid leukemia by altering the expression of a gene important for myeloid cell growth and/or differentiation, and thus this gene may function as a myeloid leukemia tumor suppressor gene. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene, and alternative translation start sites, including a non-AUG (CUG) start site, are used. [provided by RefSeq, May 2011] Transcript Variant: This variant (6) differs in the 5' UTR, lacks a portion of the 5' coding region, and uses a downstream in-frame start codon, compared to variant 1. The encoded isoform (c) is shorter at the N-terminus, compared to isoform a. Both variants 4 and 6 encode the same isoform. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. CCDS Note: This CCDS representation uses a downstream AUG start codon compared to CCDS44539.1. It is supported by the mRNA AK127209.1, which lacks the exon containing the alternative non-AUG (CUG) start codon described in PMID:10321731.

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