CD162 (SELPLG) (NM_001206609) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CD162; CLA; PSGL-1; PSGL1
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331663 representing NM_001206609. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGCAGTGGGGGCCAGTGGTCTAGAAGGAGATAAGATGGCTGGTGCCATGCCTCTGCAACTCCTCCTG TTGCTGATCCTACTGGGCCCTGGCAACAGCTTGCAGCTGTGGGACACCTGGGCAGATGAAGCCGAGAAA GCCTTGGGTCCCCTGCTTGCCCGGGACCGGAGACAGGCCACCGAATATGAGTACCTAGATTATGATTTC CTGCCAGAAACGGAGCCTCCAGAAATGCTGAGGAACAGCACTGACACCACTCCTCTGACTGGGCCTGGA ACCCCTGAGTCTACCACTGTGGAGCCTGCTGCAAGGCGTTCTACTGGCCTGGATGCAGGAGGGGCAGTC ACAGAGCTGACCACGGAGCTGGCCAACATGGGGAACCTGTCCACGGATTCAGCAGCTATGGAGATACAG ACCACTCAACCAGCAGCCACGGAGGCACAGACCACTCAACCAGTGCCCACGGAGGCACAGACCACTCCA CTGGCAGCCACAGAGGCACAGACAACTCGACTGACGGCCACGGAGGCACAGACCACTCCACTGGCAGCC ACAGAGGCACAGACCACTCCACCAGCAGCCACGGAAGCACAGACCACTCAACCCACAGGCCTGGAGGCA CAGACCACTGCACCAGCAGCCATGGAGGCACAGACCACTGCACCAGCAGCCATGGAAGCACAGACCACT CCACCAGCAGCCATGGAGGCACAGACCACTCAAACCACAGCCATGGAGGCACAGACCACTGCACCAGAA GCCACGGAGGCACAGACCACTCAACCCACAGCCACGGAGGCACAGACCACTCCACTGGCAGCCATGGAG GCCCTGTCCACAGAACCCAGTGCCACAGAGGCCCTGTCCATGGAACCTACTACCAAAAGAGGTCTGTTC ATACCCTTTTCTGTGTCCTCTGTTACTCACAAGGGCATTCCCATGGCAGCCAGCAATTTGTCCGTCAAC TACCCAGTGGGGGCCCCAGACCACATCTCTGTGAAGCAGTGCCTGCTGGCCATCCTAATCTTGGCGCTG GTGGCCACTATCTTCTTCGTGTGCACTGTGGTGCTGGCGGTCCGCCTCTCCCGCAAGGGCCACATGTAC CCCGTGCGTAATTACTCCCCCACCGAGATGGTCTGCATCTCATCCCTGTTGCCTGATGGGGGTGAGGGG CCCTCTGCCACAGCCAATGGGGGCCTGTCCAAGGCCAAGAGCCCGGGCCTGACGCCAGAGCCCAGGGAG GACCGTGAGGGGGATGACCTCACCCTGCACAGCTTCCTCCCTTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001206609
Insert Size	1287 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001206609.1
RefSeq Size	2638 bp
RefSeq ORF	1287 bp
Locus ID	6404
UniProt ID	Q14242
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Cell adhesion molecules (CAMs)
MW	44.6 kDa
Gene Summary	This gene encodes a glycoprotein that functions as a high affinity counter-receptor for the cell adhesion molecules P-, E- and L- selectin expressed on myeloid cells and stimulated T lymphocytes. As such, this protein plays a critical role in leukocyte trafficking during inflammation by tethering of leukocytes to activated platelets or endothelia expressing selectins. This protein requires two post-translational modifications, tyrosine sulfation and the addition of the sialyl Lewis x tetrasaccharide (sLex) to its O-linked glycans, for its high-affinity binding activity. Aberrant expression of this gene and polymorphisms in this gene are associated with defects in the innate and adaptive immune response. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Apr 2011] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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