AE2 (SLC4A2) (NM_001199693) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	AE2; BND3L; EPB3L1; HKB3; NBND3
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331345 representing NM_001199693. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGACTTCCTCCTGCGGCCTCAGCCAGAGCCAGAGAGCTTGGGCCCTGGGACGCCTGGGTTCCCCGAG CAGGAGGAAGACGAACTTCACCGCACCCTGGGCGTGGAGCGGTTTGAGGAGATCCTACAGGAGGCCGGG TCTCGTGGAGGGGAGGAGCCAGGCCGCAGCTATGGGGAGGAAGACTTTGAGTACCACCGCCAGTCCTCC CACCACATCCATCACCCACTGTCCACCCACCTGCCTCCGGATGCACGCCGCCGCAAGACACCCCAGGGC CCAGGACGGAAGCCTCGAAGGCGCCCGGGAGCCTCCCCGACTGGAGAAACCCCGACCATTGAGGAGGGG GAGGAAGATGAGGATGAGGCCAGCGAGGCTGAGGGGGCCCGGGCTCTCACTCAGCCGTCCCCTGTCTCC ACACCCTCCTCGGTGCAGTTCTTTCTCCAAGAGGATGACAGTGCTGACCGGAAGGCAGAGAGGACCAGT CCATCTTCCCCTGCACCACTGCCCCACCAGGAGGCGACTCCTCGGGCCTCCAAAGGGGCCCAGGCTGGA ACCCAGGTGGAGGAGGCGGAGGCGGAGGCGGTGGCGGTGGCCAGTGGCACTGCAGGGGGTGACGACGGG GGTGCCTCGGGGCGCCCCCTGCCCAAAGCCCAGCCTGGGCACCGCAGCTACAACCTTCAGGAGAGGAGG CGCATCGGGAGCATGACTGGGGCTGAGCAGGCACTGCTGCCCCGGGTCCCCACGGATGAGATTGAGGCC CAGACGCTGGCCACGGCCGACCTAGACCTCATGAAGAGTCACCGGTTTGAGGACGTTCCTGGGGTGCGG CGGCACTTGGTGCGGAAGAATGCCAAAGGTTCCACACAGAGTGGCCGAGAAGGGCGGGAGCCTGGCCCC ACACCTCGGGCCCGACCCCGGGCCCCCCACAAGCCCCATGAGGTGTTTGTGGAGCTGAATGAGTTGCTC CTGGACAAAAACCAGGAGCCCCAGTGGCGGGAGACAGCTCGCTGGATCAAATTTGAAGAGGACGTGGAG GAGGAGACTGAGCGCTGGGGGAAGCCCCACGTGGCCTCCCTCTCCTTCCGCAGTCTCCTGGAGCTCCGC AGGACCCTGGCCCATGGGGCTGTGCTCTTGGATCTGGACCAGCAGACCCTGCCCGGAGTGGCCCACCAG GTGGTGGAGCAGATGGTCATCTCTGACCAGATCAAGGCCGAGGACAGGGCCAACGTGCTGCGGGCTCTG CTGTTGAAACACAGCCACCCAAGTGATGAGAAGGACTTCTCCTTCCCCCGCAACATCTCAGCTGGCTCC CTGGGCTCCCTGCTGGGGCATCACCATGGTCAGGGGGCTGAGAGTGACCCCCACGTCACCGAGCCTCTC ATGGGAGGTGTTCCTGAGACCCGGCTGGAGGTGGAGCGAGAGCGTGAGCTGCCGCCTCCAGCACCACCA GCTGGCATCACCCGCTCCAAGTCCAAGCACGAGCTGAAACTGCTGGAGAAGATTCCTGAGAATGCCGAG GCCACGGTGGTCCTTGTGGGCTGCGTGGAGTTCCTCTCCCGCCCCACCATGGCCTTTGTGCGGCTCCGG GAGGCTGTGGAGTTGGACGCAGTGTTGGAGGTGCCGGTGCCTGTGCGTTTCCTCTTCCTGCTGCTGGGC CCGAGTAGTGCCAACATGGACTACCACGAGATCGGCCGCTCCATCTCCACCCTCATGTCAGACAAGCAA TTCCACGAGGCAGCCTACCTGGCTGACGAGCGGGAGGACCTGCTGACGGCCATCAACGCCTTCCTGGAC TGCAGCGTGGTGCTGCCGCCTTCAGAAGTGCAGGGCGAGGAGCTGCTGCGCTCTGTGGCCCACTTCCAG CGCCAGATGCTCAAGAAGCGAGAGGAGCAGGGCCGGCTGCTACCTACAGGGGCTGGGCTGGAGCCCAAA TCTGCCCAAGATAAGGCGCTCCTGCAGATGGTAGAGGCGGCAGGGGCAGCTGAAGATGATCCCCTTCGG CGGACGGGGCGGCCCTTTGGGGGGCTGATCCGAGATGTGCGGCGCCGCTATCCCCACTACCTGAGTGAC TTCCGAGATGCACTTGACCCTCAGTGCCTGGCCGCAGTCATCTTCATCTACTTTGCCGCCCTGTCTCCT GCCATCACCTTTGGGGGGCTGCTGGGAGAGAAGACGCAGGACCTGATAGGGGTGTCGGAGCTGATTATG TCCACAGCGCTCCAGGGCGTGGTCTTCTGCCTGCTGGGTGCCCAGCCCCTGTTGGTGATCGGCTTCTCA GGGCCCCTGCTGGTCTTTGAGGAGGCCTTCTTCTCGTTCTGTAGCAGCAACCACCTGGAGTACCTGGTG GGCCGTGTGTGGATCGGCTTCTGGCTGGTGTTCCTGGCCCTGCTCATGGTGGCCCTGGAGGGGAGCTTC CTGGTCCGCTTCGTCTCCCGCTTCACCCAGGAGATCTTCGCCTTCTTGATCTCACTCATCTTCATCTAT GAGACCTTCTACAAGCTGGTGAAGATCTTCCAGGAGCACCCCCTGCATGGCTGCTCAGCCTCCAACAGC TCAGAGGTGGACGGCGGTGAGAACATGACATGGGCCGGGGCAAGACCCACGCTGGGGCCGGGCAACAGG AGCTTGGCTGGGCAGTCTGGGCAGGGGAAGCCCCGGGGCCAGCCCAACACGGCCCTGCTGTCGCTGGTG CTCATGGCCGGCACCTTCTTCATCGCCTTCTTCCTGCGCAAATTCAAGAACAGCCGGTTCTTTCCTGGC CGGATCCGGCGGGTGATTGGGGACTTTGGGGTGCCCATCGCCATCCTCATCATGGTGCTTGTGGATTAC AGTATTGAGGACACCTATACCCAGAAGCTGAGCGTTCCCAGTGGATTCTCGGTGACTGCCCCAGAAAAG AGGGGCTGGGTCATCAACCCCCTGGGAGAGAAGAGCCCCTTCCCTGTGTGGATGATGGTTGCCAGCCTG CTGCCCGCCATCCTGGTCTTCATTCTCATCTTCATGGAGACACAGATCACCACGCTCATCATCTCCAAG AAGGAGCGCATGCTGCAGAAGGGCTCCGGCTTCCACCTGGACCTGCTGCTCATCGTGGCCATGGGCGGC ATCTGTGCCCTCTTTGGCCTGCCCTGGTTGGCTGCTGCCACTGTCCGCTCTGTCACTCACGCCAACGCG CTCACTGTCATGAGCAAGGCTGTGGCACCTGGGGACAAGCCCAAGATTCAGGAAGTCAAGGAGCAGCGG GTGACGGGGCTGCTGGTTGCCCTGCTTGTGGGCCTCTCCATAGTTATCGGGGATCTGCTCCGGCAGATC CCCCTGGCCGTGCTCTTTGGAATTTTCCTGTACATGGGAGTCACCTCCCTTAACGGGATCCAGTTCTAT GAGCGGCTGCATCTGCTGCTCATGCCGCCCAAACACCACCCAGATGTCACTTACGTCAAGAAGGTCCGG ACCCTCCGTATGCACCTGTTCACGGCCCTGCAGCTGCTCTGCCTGGCCCTGCTCTGGGCCGTCATGTCC ACAGCTGCCTCCCTGGCCTTCCCCTTCATCCTCATCCTCACAGTGCCGCTCCGCATGGTGGTGCTCACC CGTATCTTCACCGACCGAGAGATGAAATGTCTGGATGCTAACGAGGCAGAGCCGGTGTTTGATGAGCGG GAGGGTGTGGACGAGTACAATGAGATGCCCATGCCTGTGTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001199693
Insert Size	3699 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001199693.1
RefSeq Size	3969 bp
RefSeq ORF	3699 bp
Locus ID	6522
UniProt ID	P04920
Protein Families	Transmembrane
MW	136.2 kDa
Gene Summary	This gene encodes a member of the anion exchanger family of membrane transport proteins. The encoded protein regulates intracellular pH, biliary bicarbonate secretion, and chloride uptake. Reduced expression of this gene may be associated with primary biliary cirrhosis (PBC) in human patients, while differential expression of this gene may be associated with malignant hepatocellular carcinoma, colon and gastric cancers. [provided by RefSeq, Nov 2016] Transcript Variant: This variant (3) lacks two exons from the 5' end and contains an alternate 5' exon, as compared to variant 1. The resulting isoform (2) has a shorter and different N-terminus, as compared to isoform 1.

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