DGKZ (NM_001199267) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DAGK5; DAGK6; DGK-ZETA; hDGKzeta
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331286 representing NM_001199267. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGAGCCGCGGGACGGTAGCCCCGAGGCCCGGAGCAGCGACTCCGAGTCGGCTTCCGCCTCGTCCAGC GGCTCCGAGCGCGACGCCGGTCCCGAGCCGGACAAGGCGCCGCGGCGACTCAACAAGCGGCGCTTCCCG GGGCTGCGGCTCTTCGGGCACAGGAAAGCCATCACCAAGTCGGGCCTCCAGCACCTGGCCCCCCCTCCG CCCACCCCTGGGGCCCCGTGCAGCGAGTCAGAGCGGCAGATCCGGAGTACAGTGGACTGGAGCGAGTCA GCGACATATGGGGAGCACATCTGGTTCGAGACCAACGTGTCCGGGGACTTCTGCTACGTTGGGGAGCAG TACTGTGTAGCCAGGATGCTGAAGTCAGTGTCTCGAAGAAAGTGCGCAGCCTGCAAGATTGTGGTGCAC ACGCCCTGCATCGAGCAGCTGGAGAAGATAAATTTCCGCTGTAAGCCGTCCTTCCGTGAATCAGGCTCC AGGAATGTCCGCGAGCCAACCTTTGTACGGCACCACTGGGTACACAGACGACGCCAGGACGGCAAGTGT CGGCACTGTGGGAAGGGATTCCAGCAGAAGTTCACCTTCCACAGCAAGGAGATTGTGGCCATCAGCTGC TCGTGGTGCAAGCAGGCATACCACAGCAAGGTGTCCTGCTTCATGCTGCAGCAGATCGAGGAGCCGTGC TCGCTGGGGGTCCACGCAGCCGTGGTCATCCCGCCCACCTGGATCCTCCGCGCCCGGAGGCCCCAGAAT ACTCTGAAAGCAAGCAAGAAGAAGAAGAGGGCATCCTTCAAGAGGAAGTCCAGCAAGAAAGGGCCTGAG GAGGGCCGCTGGAGACCCTTCATCATCAGGCCCACCCCCTCCCCGCTCATGAAGCCCCTGCTGGTGTTT GTGAACCCCAAGAGTGGGGGCAACCAGGGTGCAAAGATCATCCAGTCTTTCCTCTGGTATCTCAATCCC CGACAAGTCTTCGACCTGAGCCAGGGAGGGCCCAAGGAGGCGCTGGAGATGTACCGCAAAGTGCACAAC CTGCGGATCCTGGCGTGCGGGGGCGACGGCACGGTGGGCTGGATCCTCTCCACCCTGGACCAGCTACGC CTGAAGCCGCCACCCCCTGTTGCCATCCTGCCCCTGGGTACTGGCAACGACTTGGCCCGAACCCTCAAC TGGGGTGGGGGCTACACAGATGAGCCTGTGTCCAAGATCCTCTCCCACGTGGAGGAGGGGAACGTGGTA CAGCTGGACCGCTGGGACCTCCACGCTGAGCCCAACCCCGAGGCAGGGCCTGAGGACCGAGATGAAGGC GCCACCGACCGGTTGCCCCTGGATGTCTTCAACAACTACTTCAGCCTGGGCTTTGACGCCCACGTCACC CTGGAGTTCCACGAGTCTCGAGAGGCCAACCCAGAGAAATTCAACAGCCGCTTTCGGAATAAGATGTTC TACGCCGGGACAGCTTTCTCTGACTTCCTGATGGGCAGCTCCAAGGACCTGGCCAAGCACATCCGAGTG GTGTGTGATGGAATGGACTTGACTCCCAAGATCCAGGACCTGAAACCCCAGTGTGTTGTTTTCCTGAAC ATCCCCAGGTACTGTGCGGGCACCATGCCCTGGGGCCACCCTGGGGAGCACCACGACTTTGAGCCCCAG CGGCATGACGACGGCTACCTCGAGGTCATTGGCTTCACCATGACGTCGTTGGCCGCGCTGCAGGTGGGC GGACACGGCGAGCGGCTGACGCAGTGTCGCGAGGTGGTGCTCACCACATCCAAGGCCATCCCGGTGCAG GTGGATGGCGAGCCCTGCAAGCTTGCAGCCTCACGCATCCGCATCGCCCTGCGCAACCAGGCCACCATG GTGCAGAAGGCCAAGCGGCGGAGCGCCGCCCCCCTGCACAGCGACCAGCAGCCGGTGCCAGAGCAGTTG CGCATCCAGGTGAGTCGCGTCAGCATGCACGACTATGAGGCCCTGCACTACGACAAGGAGCAGCTCAAG GAGGCCTCTGTGCCGCTGGGCACTGTGGTGGTCCCAGGAGACAGTGACCTAGAGCTCTGCCGTGCCCAC ATTGAGAGACTCCAGCAGGAGCCCGATGGTGCTGGAGCCAAGTCCCCGACATGCCAGAAACTGTCCCCC AAGTGGTGCTTCCTGGACGCCACCACTGCCAGCCGCTTCTACAGGATCGACCGAGCCCAGGAGCACCTC AACTATGTGACTGAGATCGCACAGGATGAGATTTATATCCTGGACCCTGAGCTGCTGGGGGCATCGGCC CGGCCTGACCTCCCAACCCCCACTTCCCCTCTCCCCACCTCACCCTGCTCACCCACGCCCCGGTCACTG CAAGGGGATGCTGCACCCCCTCAAGGTGAAGAGCTGATTGAGGCTGCCAAGAGGAACGACTTCTGTAAG CTCCAGGAGCTGCACCGAGCTGGGGGCGACCTCATGCACCGAGACGAGCAGAGTCGCACGCTCCTGCAC CACGCAGTCAGCACTGGCAGCAAGGATGTGGTCCGCTACCTGCTGGACCACGCCCCCCCAGAGATCCTT GATGCGGTGGAGGAAAACGGGGAGACCTGTTTGCACCAAGCAGCGGCCCTGGGCCAGCGCACCATCTGC CACTACATCGTGGAGGCCGGGGCCTCGCTCATGAAGACAGACCAGCAGGGCGACACTCCCCGGCAGCGG GCTGAGAAGGCTCAGGACACCGAGCTGGCCGCCTACCTGGAGAACCGGCAGCACTACCAGATGATCCAG CGGGAGGACCAGGAGACGGCTGTGTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001199267
Insert Size	2787 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001199267.1
RefSeq Size	3656 bp
RefSeq ORF	2787 bp
Locus ID	8525
UniProt ID	Q13574
Protein Families	Druggable Genome
Protein Pathways	Glycerolipid metabolism, Glycerophospholipid metabolism, Metabolic pathways, Phosphatidylinositol signaling system
MW	104 kDa
Gene Summary	The protein encoded by this gene belongs to the eukaryotic diacylglycerol kinase family. It may attenuate protein kinase C activity by regulating diacylglycerol levels in intracellular signaling cascade and signal transduction. Alternative splicing occurs at this locus and multiple transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Nov 2010] Transcript Variant: This variant (6) has an alternate 5' sequence including the coding region compared to variant 4. The resulting isoform (6) has a shorter and distinct N-terminus, compared to isoform 4.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.