CYP4F3 (NM_001199209) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CPF3; CYP4F; CYPIVF3; LTB4H
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331267 representing NM_001199209. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGCCACAGCTGAGCCTGTCCTCGCTGGGCCTTTGGCCAATGGCAGCATCCCCGTGGCTGCTCCTGCTG CTGGTTGGGGCCTCCTGGCTCCTGGCCCGCATCCTGGCCTGGACCTATACCTTCTATGACAACTGCTGC CGCCTCCGGTGTTTCCCGCAACCCCCGAAACGGAATTGGTTCTTGGGTCACCTGGGCCTGGTCACCCCC ACGGAGCAGGGCATGAGGGTCCTGACTCAGCTGGTGGCCACCTACCCCCAGGGCTTTAAGGTCTGGATG GGCCCCATCTTCCCCGTCATCCGTTTTTGCCACCCCAACATCATCCGGTCTGTCATCAACGCCTCAGCT GCCATTGTACCAAAGGACAAGGTCTTCTACAGCTTCCTGAAGCCCTGGCTGGGGGATGGGCTCCTGCTG AGTGCTGGTGAAAAGTGGAGCCGCCACCGTCGGATGCTGACGCCTGCCTTCCATTTCAACATCCTGAAG CCCTATATGAAGATTTTCAATGAGAGTGTGAACATCATGCATGCCAAGTGGCAGCTCCTGGCCTCAGAG GGTAGTGCCCGTCTGGACATGTTTGAGCACATCAGCCTCATGACCTTGGACAGTCTGCAGAAATGTGTC TTCAGCTTTGACAGCCATTGCCAGGAGAAGCCCAGTGAATATATTGCCGCCATCTTGGAGCTCAGTGCC CTTGTGACAAAAAGACACCAGCAGATCCTCCTGTACATAGACTTCCTGTATTATCTCACCCCTGATGGG CAGCGTTTCCGCAGGGCCTGCCGCCTGGTGCACGACTTCACAGATGCCGTCATCCAGGAGCGGCGCCGC ACCCTCCCTAGCCAGGGTGTTGATGACTTCCTCCAAGCCAAGGCCAAATCCAAGACTTTGGACTTCATT GATGTACTCCTGCTGAGCAAGGATGAAGATGGGAAGAAGTTGTCCGATGAGGACATAAGAGCAGAAGCT GACACCTTTATGTTTGAGGGCCATGACACCACAGCCAGTGGTCTCTCCTGGGTCCTGTACCACCTTGCA AAGCACCCGGAATACCAGGAGCGCTGTCGGCAGGAGGTGCAAGAGCTTCTGAAGGACCGTGAGCCTAAA GAGATTGAATGGGACGACCTGGCCCAGCTGCCCTTCCTGACCATGTGCATTAAGGAGAGCCTGAGGCTG CATCCCCCAGTCCCTGCCGTCTCTCGCTGCTGCACCCAAGACATTGTGCTCCCAGACGGCCGGGTCATC CCCAAAGGCATTATCTGCCTCATCAGTGTTTTTGGAACCCATCACAACCCAGCCGTGTGGCCGGACCCT GAGGTCTATGACCCCTTTCGCTTTGACCCAAAGAACATCAAGGAGAGGTCACCTCTGGCTTTTATTCCC TTCTCAGCAGGGCCCAGGAACTGCATCGGGCAGGCGTTCGCGATGGCGGAGATGAAGGTGGTCCTGGGG CTCACGCTGCTGCGCTTCCGCGTCCTGCCTGACCACACCGAGCCCCGCAGGAAGCCGGAGCTGGTCCTG CGCGCAGAGGGCGGACTTTGGCTGCGGGTGGAGCCCCTGAGCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001199209
Insert Size	1563 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001199209.1
RefSeq Size	2972 bp
RefSeq ORF	1563 bp
Locus ID	4051
UniProt ID	Q08477
Protein Families	Druggable Genome, P450, Transmembrane
Protein Pathways	Arachidonic acid metabolism, Metabolic pathways
MW	59.7 kDa
Gene Summary	This gene, CYP4F3, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. The enzyme starts the process of inactivating and degrading leukotriene B4, a potent mediator of inflammation. This gene is part of a cluster of cytochrome P450 genes on chromosome 19. Another member of this family, CYP4F8, is approximately 18 kb away. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Apr 2019] Transcript Variant: This variant (3) differs in the 5' UTR, lacks an alternate in-frame exon, and contains another alternate in-frame exon compared to variant 1. The resulting isoform (b) is the same length but has a different internal segment compared to isoform a. Variants 2, 3, and 4 all encode the same isoform (b).

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