USP19 (NM_001199161) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ZMYND9
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331254 representing NM_001199161. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGTCTGGCGGGGCCAGTGCCACAGGCCCAAGGAGAGGGCCCCCAGGACTGGAGGACACCACTAGTAAG AAGAAGCAGAAGGATCGAGCAAACCAGGAGAGCAAGGATGGAGATCCTAGGAAAGAGACAGGGTCTCGA TATGTTGCCCAGGCTGGTCTTGAACCTCTGGCCTCAGGTGATCCTTCTGCCTCAGCCTCCCATGCAGCT GGGATCACAGGCTCACGCCACCGTACCCGGCTGTTCTTTCCTTCATCGTCAGGGTCAGCATCCACTCCT CAAGAGGAGCAGACCAAAGAGGGAGCTTGTGAAGACCCTCATGATCTCTTGGCTACTCCCACTCCAGAG TTGTTGCTCGATTGGAGGCAGAGTGCAGAAGAGGTGATTGTCAAGCTTCGTGTGGGAGTAGGTCCCCTG CAGCTGGAGGATGTAGATGCTGCTTTCACAGATACAGACTGTGTGGTGCGGTTTGCAGGTGGTCAGCAG TGGGGTGGTGTCTTCTATGCTGAGATAAAAAGCTCTTGTGCTAAAGTGCAAACCCGCAAGGGCAGTCTC CTGCACCTGACACTGCCCAAAAAGGTGCCTATGCTCACGTGGCCCTCCCTCCTGAAGAAACCTCTAGGG ACCCAGGAGCTGGTGCCGGGGCTGCGGTGCCAGGAGAATGGGCAGGAACTGTCTCCCATTGCCCTGGAG CCAGGCCCTGAGCCCCACCGGGCTAAGCAGGAGGCCCGGAACCAGAAGCGGGCCCAGGGCCGTGGTGAG GTAGGCGCAGGGGCTGGCCCCGGGGCCCAGGCAGGGCCCAGCGCCAAGAGGGCTGTGCATCTCTGCAGA GGGCCAGAGGGGGACGGGTCCAGGGATGACCCTGGACCCCGGGGTGATGCCCCACCCTTCGTGGCTGAC CCAGCCACCCAGGTTGAGGCTGATGAACAGCTTTGCATACCACCGCTGAACTCCCAAACCTGCCTCCTG GGCTCAGAGGAGAATTTAGCCCCTTTGGCAGGAGAGAAAGCAGTGCCTCCCGGGAATGACCCAGTCTCT CCAGCCATGGTCCGGAGCAGAAACCCTGGGAAAGATGACTGTGCCAAGGAGGAGATGGCAGTGGCAGCA GATGCTGCAACCTTGGTGGATGAGCCCGAGTCGATGGTGAACCTGGCGTTTGTCAAGAATGACTCGTAT GAGAAGGGCCCGGATTCAGTGGTGGTGCACGTGTACGTGAAGGAGATCTGCAGGGACACCTCAAGAGTA CTTTTCCGTGAGCAGGACTTCACGCTCATCTTCCAGACCAGGGATGGAAACTTCCTGAGGCTGCACCCG GGCTGTGGGCCCCACACCACCTTCCGTTGGCAGGTGAAGCTCAGGAATCTGATTGAGCCAGAGCAGTGC ACCTTCTGTTTCACGGCTTCTCGCATCGACATCTGCCTTCGTAAGAGGCAGAGTCAGCGCTGGGGGGGC CTGGAGGCCCCGGCTGCACGAGGTGCAGTGGGTGGTGCAAAGGTTGCCGTGCCGACAGGTCCAACCCCT CTGGATTCAACCCCACCAGGAGGTGCTCCCCACCCCCTGACAGGCCAGGAGGAGGCCCGGGCTGTGGAG AAGGATAAATCCAAGGCACGATCTGAGGACACAGGGCTAGACAGTGTGGCAACCCGCACACCCATGGAG CATGTAACCCCAAAGCCAGAGACACACCTGGCCTCGCCCAAGCCTACATGCATGGTGCCTCCCATGCCC CACAGCCCAGTTAGTGGAGACAGCGTGGAGGAGGAGGAAGAGGAAGAGAAGAAGGTGTGTCTGCCAGGC TTCACTGGCCTTGTCAATTTAGGCAACACCTGCTTCATGAACAGCGTCATTCAGTCTCTGTCCAACACT CGGGAACTCCGGGACTTCTTCCATGACCGCTCCTTTGAGGCTGAGATCAACTACAACAACCCACTAGGG ACTGGTGGGCGTCTGGCCATTGGCTTTGCCGTGCTGCTTCGGGCGCTGTGGAAGGGCACCCACCATGCC TTCCAGCCTTCCAAGTTGAAGGCCATTGTGGCGAGTAAGGCCAGCCAGTTCACAGGCTATGCACAGCAT GATGCCCAGGAGTTCATGGCTTTCCTGCTGGATGGGCTGCACGAGGACCTGAATCGCATTCAGAACAAG CCCTACACAGAGACCGTGGATTCAGATGGGCGGCCCGATGAGGTGGTAGCTGAGGAAGCATGGCAGCGG CACAAGATGAGGAATGACTCTTTCATCGTGGACCTATTTCAGGGGCAGTACAAGTCGAAGCTGGTGTGC CCTGTGTGTGCCAAGGTCTCCATCACTTTTGACCCGTTTCTTTATCTGCCGGTGCCCTTGCCACAAAAG CAAAAGGTTCTCCCTGTCTTTTATTTTGCCCGAGAGCCCCACAGCAAGCCCATCAAGTTCCTGGTGAGC GTCAGCAAGGAGAACTCCACTGCGAGCGAAGTATTGGACTCCCTCTCTCAGAGTGTTCATGTGAAGCCT GAGAACCTGCGTTTGGCGGAGGTAATTAAGAATCGTTTTCATCGTGTGTTCCTACCCTCCCACTCACTG GACACTGTGTCCCCATCTGATACGCTCCTCTGCTTTGAGCTGCTATCCTCAGAGTTGGCTAAGGAGCGG GTAGTGGTGCTAGAGGTGCAACAGCGCCCCCAGGTGCCCAGCGTCCCCATCTCCAAGTGTGCAGCCTGC CAGCGGAAGCAACAGTCGGAGGATGAAAAGCTGAAGCGCTGTACCCGGTGCTACCGTGTGGGCTACTGC AACCAGCTCTGCCAGAAAACCCACTGGCCTGACCACAAGGGCCTCTGCCGACCTGAGAACATTGGCTAC CCCTTCCTGGTCAGTGTACCTGCCTCACGCCTCACTTATGCCCGCCTCGCTCAGTTGCTAGAGGGCTAT GCCCGGTACTCTGTGAGTGTATTCCAGCCACCCTTTCAGCCAGGCCGCATGGCCTTGGAGTCTCAGAGC CCTGGCTGCACCACACTGCTCTCCACAGGTTCCCTGGAGGCTGGGGACAGCGAGAGAGACCCCATTCAG CCACCTGAGCTCCAGCTGGTGACCCCTATGGCTGAGGGGGACACAGGGCTTCCCCGGGTGTGGGCAGCC CCTGACCGGGGTCCTGTGCCCAGCACCAGTGGAATTTCTTCTGAGATGCTGGCCAGTGGGCCCATTGAG GTTGGCTCCTTGCCAGCTGGCGAGAGGGTGTCCCGACCCGAAGCTGCTGTGCCTGGGTACCAGCATCCA AGTGAAGCTATGAATGCCCACACACCCCAGTTCTTCATCTATAAAATTGATTCATCCAACCGAGAGCAG CGGCTAGAGGACAAAGGAGACACCCCACTGGAGCTGGGTGACGACTGTAGCCTGGCTCTCGTCTGGCGG AACAATGAGCGCTTGCAGGAGTTTGTGTTGGTAGCCTCCAAGGAGCTGGAATGTGCTGAGGATCCAGGC TCTGCCGGTGAGGCTGCCCGGGCCGGCCACTTCACCCTGGACCAGTGCCTCAACCTCTTCACACGGCCT GAGGTGCTGGCACCCGAGGAGGCCTGGTACTGCCCACAGTGCAAACAGCACCGTGAGGCCTCCAAGCAG CTGTTGCTATGGCGCCTGCCAAATGTTCTCATCGTGCAGCTCAAGCGCTTCTCCTTTCGTAGTTTTATC TGGCGTGACAAGATCAATGACTTGGTGGAGTTCCCTGTTAGGAACCTGGACCTGAGCAAGTTCTGCATT GGTCAGAAAGAGGAGCAGCTGCCCAGCTACGATCTATATGCTGTCATCAACCACTATGGAGGCATGATT GGTGGCCACTACACTGCCTGTGCACGCCTGCCCAATGATCGTAGCAGTCAGCGCAGTGACGTGGGCTGG CGCTTGTTTGATGACAGCACAGTGACAACGGTAGACGAGAGCCAGGTTGTGACGCGTTATGCCTATGTA CTCTTCTACCGCCGGCGGAACTCTCCTGTGGAGAGGCCCCCCAGGGCAGGTCACTCTGAGCACCACCCA GACCTAGGCCCTGCAGCTGAGGCTGCTGCCAGCCAGGGACTAGGCCCTGGCCAGGCCCCCGAGGTGGCC CCCACGCGGACAGCCCCTGAACGCTTCGCCCCCCCTGTGGATCGGCCAGCCCCCACCTACAGCAACATG GAGGAGGTGGATTAG
Restriction Sites	SgfI-RsrII
ACCN	NM_001199161
Insert Size	4155 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001199161.1
RefSeq Size	4883 bp
RefSeq ORF	4155 bp
Locus ID	10869
UniProt ID	O94966
Protein Families	Druggable Genome, Protease, Transmembrane
MW	151.9 kDa
Gene Summary	Protein ubiquitination controls many intracellular processes, including cell cycle progression, transcriptional activation, and signal transduction. This dynamic process, involving ubiquitin conjugating enzymes and deubiquitinating enzymes, adds and removes ubiquitin. Deubiquitinating enzymes are cysteine proteases that specifically cleave ubiquitin from ubiquitin-conjugated protein substrates. This protein is a ubiquitin protein ligase and plays a role in muscle wasting. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2017] Transcript Variant: This variant (2) has an alternate splice site in the CDS and an alternate 3' exon, as compared to variant 1. The resulting isoform (2) has an internal additional 2 aa and a shorter and distinct C-terminus, as compared to isoform 1.

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