NADK (NM_001198993) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	dJ283E3.1
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331234 representing NM_001198993. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGAAATGGAACAAGAAAAAATGACCATGAATAAGGAATTGAGTCCAGACGCGGCTGCTTACTGCTGC TCGGCCTGCCACGGCGATGAGACCTGGAGTTACAACCACCCCATCCGGGGCCGGGCCAAGTCTCGCAGC CTGTCTGCCTCGCCCGCCCTGGGGAGCACCAAGGAGTTCAGGAGGACACGCTCTCTTCATGGGCCATGC CCGGTGACCACTTTTGGACCAAAGGCCTGTGTGCTGCAGAACCCCCAGACCATCATGCACATTCAGGAC CCCGCGAGCCAGCGGCTGACGTGGAACAAGTCCCCAAAGAGCGTCCTTGTCATCAAGAAGATGAGAGAT GCCAGCCTACTGCAGCCGTTCAAGGAGCTCTGCACGCACCTCATGGAGGAGAACATGATCGTGTATGTG GAAAAGAAAGTGCTAGAAGACCCTGCCATCGCCAGCGATGAAAGCTTTGGGGCAGTGAAGAAGAAATTC TGTACCTTTCGAGAAGATTATGATGACATTTCCAATCAGATAGACTTCATCATCTGCCTGGGGGGAGAC GGGACGCTGCTGTACGCTTCCTCGCTTTTCCAGGGCAGCGTCCCTCCGGTCATGGCCTTCCACCTGGGC TCCCTGGGCTTCCTGACCCCATTCAGCTTTGAGAACTTTCAGTCCCAAGTTACTCAGGTGATAGAGGGG AACGCAGCTGTTGTTCTCCGGAGTCGGCTGAAGGTCAGGGTGGTGAAGGAGCTCCGGGGGAAGAAGACG GCCGTGCACAATGGGCTGGGTGAGAACGGCTCGCAGGCTGCAGGCCTGGACATGGATGTCGGGAAGCAG GCCATGCAGTACCAGGTCCTGAATGAGGTGGTGATTGACAGAGGCCCCTCCTCCTACCTGTCCAATGTG GATGTCTACCTGGACGGACACCTCATCACCACGGTGCAGGGCGACGGAGTGATCGTGTCCACCCCGACG GGCAGCACGGCGTATGCGGCCGCGGCCGGGGCCTCCATGATCCACCCCAACGTGCCGGCCATCATGATC ACGCCCATCTGCCCCCACTCGCTGTCCTTCCGGCCCATCGTGGTCCCCGCAGGGGTCGAGCTGAAGATC ATGCTGTCACCTGAAGCAAGGAACACAGCATGGGTGTCCTTTGATGGACGGAAGAGACAAGAGATCCGC CATGGAGACAGCATCAGCATCACTACCTCATGCTACCCGCTCCCCTCCATCTGTGTGCGGGACCCCGTG AGCGACTGGTTTGAGAGCCTCGCCCAGTGCCTGCATTGGAACGTCCGGAAGAAGCAAGCCCACTTCGAG GAGGAGGAGGAGGAGGAGGAGGAGGGCTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001198993
Insert Size	1341 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001198993.1
RefSeq Size	3175 bp
RefSeq ORF	1341 bp
Locus ID	65220
UniProt ID	O95544
Protein Families	Druggable Genome
Protein Pathways	Metabolic pathways, Nicotinate and nicotinamide metabolism
MW	49.2 kDa
Gene Summary	NADK catalyzes the transfer of a phosphate group from ATP to NAD to generate NADP, which in its reduced form acts as an electron donor for biosynthetic reactions (Lerner et al., 2001 [PubMed 11594753]).[supplied by OMIM, Mar 2008] Transcript Variant: This variant (2) has an alternate 5' UTR compared to variant 1. Variants 1, 2 and 5 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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