RUNX1T1 (NM_001198631) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	AML1-MTG8; AML1T1; CBFA2T1; CDR; ETO; MTG8; ZMYND2
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331216 representing NM_001198631. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGATATCTGTCAAAAGAAACACTTGGAGAGCACTGAGTTTAGTAATAGGTGACTGCCGGAAAAAAGGG AACTTTGAATATTGTCAAGATCGTACTGAGAAGCACTCCACAATGCCAGACTCACCTGTGGATGTGAAG ACGCAATCTAGGCTGACTCCTCCAACAATGCCACCTCCCCCAACTACTCAAGGAGCTCCAAGAACCAGT TCATTTACACCGACAACGTTAACTAATGGCACGAGCCATTCTCCTACAGCCTTGAATGGCGCCCCCTCA CCACCCAATGGCTTCAGCAATGGGCCTTCCTCTTCTTCCTCCTCCTCTCTGGCTAATCAACAGCTGCCC CCAGCCTGTGGTGCCAGGCAACTCAGCAAGCTGAAAAGGTTCCTTACTACCCTGCAGCAGTTTGGCAAT GACATTTCACCCGAGATAGGAGAAAGAGTTCGCACCCTCGTTCTGGGACTAGTGAACTCCACTTTGACA ATTGAAGAATTTCATTCCAAACTGCAAGAAGCTACTAACTTCCCACTGAGACCTTTTGTCATCCCATTT TTGAAGGCCAACTTGCCCCTGCTGCAGCGTGAGCTCCTCCACTGCGCAAGACTGGCCAAACAGAACCCT GCCCAGTACCTCGCCCAGCATGAACAGCTGCTTCTGGATGCCAGCACCACCTCACCTGTTGACTCCTCA GAGCTGCTTCTCGATGTGAACGAAAACGGGAAGAGGCGAACTCCAGACAGAACCAAAGAAAATGGCTTT GACAGAGAGCCTTTGCACTCAGAACATCCAAGCAAGCGACCATGCACTATTAGCCCAGGCCAGCGGTAC AGTCCAAATAACGGCTTATCCTACCAGCCCAATGGCCTGCCTCACCCTACCCCACCTCCACCTCAGCAT TACCGTTTGGATGATATGGCCATTGCCCACCACTACAGGGACTCCTATCGACACCCCAGCCACAGGGAC CTCAGGGACAGAAACAGACCTATGGGGTTGCATGGCACACGTCAAGAAGAAATGATTGATCACAGACTA ACAGACAGAGAATGGGCAGAAGAGTGGAAACATCTTGACCATCTGTTAAACTGCATAATGGACATGGTA GAAAAAACAAGGCGATCTCTCACCGTACTAAGGCGGTGTCAAGAAGCAGACCGGGAAGAATTGAATTAC TGGATCCGGCGGTACAGTGACGCCGAGGACTTAAAAAAAGGTGGCGGCAGTAGCAGCAGCCACTCTAGG CAGCAGAGTCCCGTCAACCCAGACCCAGTTGCACTAGACGCGCATCGGGAATTCCTTCACAGGCCTGCG TCTGGATACGTGCCAGAGGAGATCTGGAAGAAAGCTGAGGAGGCCGTCAATGAGGTGAAGCGCCAGGCG ATGACGGAGCTGCAGAAGGCCGTGTCTGAGGCGGAGCGGAAAGCCCACGACATGATCACAACAGAGAGG GCCAAGATGGAGCGCACGGTCGCCGAGGCCAAACGGCAGGCGGCGGAGGACGCACTGGCAGTTATCAAT CAGCAGGAGGATTCAAGCGAGAGTTGCTGGAATTGTGGCCGTAAAGCGAGTGAAACCTGCAGTGGCTGT AACACAGCCCGATACTGTGGCTCATTTTGCCAGCACAAAGACTGGGAGAAGCACCATCACATCTGTGGA CAGACCCTGCAGGCCCAGCAGCAGGGAGACACACCTGCAGTCAGCTCCTCTGTCACGCCCAACAGCGGG GCTGGGAGCCCGATGGACACACCACCAGCAGCCACTCCGAGGTCAACCACCCCGGGAACCCCTTCCACC ATAGAGACAACCCCTCGCTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001198631
Insert Size	1815 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001198631.1
RefSeq Size	7483 bp
RefSeq ORF	1815 bp
Locus ID	862
UniProt ID	Q06455
Protein Families	Transcription Factors
Protein Pathways	Acute myeloid leukemia, Pathways in cancer
MW	67.6 kDa
Gene Summary	This gene encodes a member of the myeloid translocation gene family which interact with DNA-bound transcription factors and recruit a range of corepressors to facilitate transcriptional repression. The t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities in acute myeloid leukemia. The translocation produces a chimeric gene made up of the 5'-region of the runt-related transcription factor 1 gene fused to the 3'-region of this gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2010] Transcript Variant: This variant (12) utilizes two alternate exons in the 5' UTR and coding region compared to variant 1. The resulting protein (isoform B, also known as MTG8b) has a longer and distinct N-terminus, compared to isoform A. Variants 2 and 7-12 all encode isoform B. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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