EIF4G1 (NM_001194947) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	EIF-4G1; EIF4F; EIF4G; EIF4GI; P220; PARK18
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331169 representing NM_001194947. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGAACAAAGCTCCACAGTCCACAGGCCCCCCACCCGCCCCATCCCCCGGACTCCCACAGCCAGCGTTT CCCCCGGGGCAGACAGCGCCGGTGGTGTTCAGTACGCCACAAGCGACACAAATGAACACGCCTTCTCAG CCCCGCCAGGGAGGATTCAGGTCTCTGCAGCACTTCTACCCTAGCCGGGCCCAGCCCCCGAGCAGTGCA GCCTCCCGAGTGCAGAGTGCAGCCCCTGCCCGCCCTGGCCCAGCTGCCCATGTCTACCCTGCTGGATCC CAAGTAATGATGATCCCTTCCCAGATCTCCTACCCAGCCTCCCAGGGGGCCTACTACATCCCTGGACAG GGGCGTTCCACATACGTTGTCCCGACACAGCAGTACCCTGTGCAGCCAGGAGCCCCAGGCTTCTATCCA GGTGCAAGCCCTACAGAATTTGGGACCTACGCTGGCGCCTACTATCCAGCCCAAGGGGTGCAGCAGTTT CCCACTGGCGTGGCCCCCGCCCCAGTTTTGATGAACCAGCCACCCCAGATTGCTCCCAAGAGGGAGCGT AAGACGATCCGAATTCGAGATCCAAACCAAGGAGGAAAGGATATCACAGAGGAGATCATGTCTGGGGCC CGCACTGCCTCCACACCCACCCCTCCCCAGACGGGAGGCGGTCTGGAGCCTCAAGCTAATGGGGAGACG CCCCAGGTTGCTGTCATTGTCCGGCCAGATGACCGGTCACAGGGAGCAATCATTGCTGACCGGCCAGGG CTGCCTGGCCCAGAGCATAGCCCTTCAGAATCCCAGCCTTCGTCGCCTTCTCCGACCCCATCACCATCC CCAGTCTTGGAACCGGGGTCTGAGCCTAATCTCGCAGTCCTCTCTATTCCTGGGGACACTATGACAACT ATACAAATGTCTGTAGAAGAATCAACCCCCATCTCCCGTGAAACTGGGGAGCCATATCGCCTCTCTCCA GAACCCACTCCTCTCGCCGAACCCATACTGGAAGTAGAAGTGACACTTAGCAAACCGGTTCCAGAATCT GAGTTTTCTTCCAGTCCTCTCCAGGCTCCCACCCCTTTGGCATCTCACACAGTGGAAATTCATGAGCCT AATGGCATGGTCCCATCTGAAGATCTGGAACCAGAGGTGGAGTCAAGCCCAGAGCTTGCTCCTCCCCCA GCTTGCCCCTCCGAATCCCCTGTGCCCATTGCTCCAACTGCCCAACCTGAGGAACTGCTCAACGGAGCC CCCTCGCCACCAGCTGTGGACTTAAGCCCAGTCAGTGAGCCAGAGGAGCAGGCCAAGGAGGTGACAGCA TCAATGGCGCCCCCCACCATCCCCTCTGCTACTCCAGCTACGGCTCCTTCAGCTACTTCCCCAGCTCAG GAGGAGGAAATGGAAGAAGAAGAAGAAGAGGAAGAAGGAGAAGCAGGAGAAGCAGGAGAAGCTGAGAGT GAGAAAGGAGGAGAGGAACTGCTCCCCCCAGAGAGTACCCCTATTCCAGCCAACTTGTCTCAGAATTTG GAGGCAGCAGCAGCCACTCAAGTGGCAGTATCTGTGCCAAAGAGGAGACGGAAAATTAAGGAGCTAAAT AAGAAGGAGGCTGTTGGAGACCTTCTGGATGCCTTCAAGGAGGCGAACCCGGCAGTACCAGAGGTGGAA AATCAGCCTCCTGCAGGCAGCAATCCAGGCCCAGAGTCTGAGGGCAGTGGTGTGCCCCCACGTCCTGAG GAAGCAGATGAGACCTGGGACTCAAAGGAAGACAAAATTCACAATGCTGAGAACATCCAGCCCGGGGAA CAGAAGTATGAATATAAGTCAGATCAGTGGAAGCCTCTAAACCTAGAGGAGAAAAAACGTTACGACCGT GAGTTCCTGCTTGGTTTTCAGTTCATCTTTGCCAGTATGCAGAAGCCAGAGGGATTGCCACATATCAGT GACGTGGTGCTGGACAAGGCCAATAAAACACCACTGCGGCCACTGGATCCCACTAGACTACAAGGCATA AATTGTGGCCCAGACTTCACTCCATCCTTTGCCAACCTTGGCCGGACAACCCTTAGCACCCGTGGGCCC CCAAGGGGTGGGCCAGGTGGGGAGCTGCCCCGTGGGCCGGCTGGCCTGGGACCCCGGCGCTCTCAGCAG GGACCCCGAAAAGAACCACGCAAGATCATTGCCACAGTGTTAATGACCGAAGATATAAAACTGAACAAA GCAGAGAAAGCCTGGAAACCCAGCAGCAAGCGGACGGCGGCTGATAAGGATCGAGGGGAAGAAGATGCT GATGGCAGCAAAACCCAGGACCTATTCCGCAGGGTGCGCTCCATCCTGAATAAACTGACACCCCAGATG TTCCAGCAGCTGATGAAGCAAGTGACGCAGCTGGCCATCGACACCGAGGAACGCCTCAAAGGGGTCATT GACCTCATTTTTGAGAAGGCCATTTCAGAGCCCAACTTCTCTGTGGCCTATGCCAACATGTGCCGCTGC CTCATGGCGCTGAAAGTGCCCACTACGGAAAAGCCAACAGTGACTGTGAACTTCCGAAAGCTGTTGTTG AATCGATGTCAGAAGGAGTTTGAGAAAGACAAAGATGATGATGAGGTTTTTGAGAAGAAGCAAAAAGAG ATGGATGAAGCTGCTACGGCAGAGGAACGAGGACGCCTGAAGGAAGAGCTGGAAGAGGCTCGGGACATA GCCCGGCGGCGCTCTTTAGGGAATATCAAGTTTATTGGAGAGTTGTTCAAACTGAAGATGTTAACAGAG GCAATAATGCATGACTGTGTGGTCAAACTGCTTAAGAACCATGATGAAGAGTCCCTTGAGTGCCTTTGT CGTCTGCTCACCACCATTGGCAAAGACCTGGACTTTGAAAAAGCCAAGCCCCGAATGGATCAGTATTTC AACCAGATGGAAAAAATCATTAAAGAAAAGAAGACGTCATCCCGCATCCGCTTTATGCTGCAGGACGTG CTGGATCTGCGAGGGAGCAATTGGGTGCCACGCCGAGGGGATCAGGGTCCCAAGACCATTGACCAGATC CATAAGGAGGCTGAGATGGAAGAACATCGAGAGCACATCAAAGTGCAGCAGCTCATGGCCAAGGGCAGT GACAAGCGTCGGGGCGGTCCTCCAGGCCCTCCCATCAGCCGTGGACTTCCCCTTGTGGATGATGGTGGC TGGAACACAGTTCCCATCAGCAAAGGTAGCCGCCCCATTGACACCTCACGACTCACCAAGATCACCAAG CCTGGCTCCATCGATTCTAACAACCAGCTCTTTGCACCTGGAGGGCGACTGAGCTGGGGCAAGGGCAGC AGCGGAGGCTCAGGAGCCAAGCCCTCAGACGCAGCATCAGAAGCTGCTCGCCCAGCTACTAGTACTTTG AATCGCTTCTCAGCCCTTCAACAAGCGGTACCCACAGAAAGCACAGATAATAGACGTGTGGTGCAGAGG AGTAGCTTGAGCCGAGAACGAGGCGAGAAAGCTGGAGACCGAGGAGACCGCCTAGAGCGGAGTGAACGG GGAGGGGACCGTGGGGACCGGCTTGATCGTGCGCGGACACCTGCTACCAAGCGGAGCTTCAGCAAGGAA GTGGAGGAGCGGAGTAGAGAACGGCCCTCCCAGCCTGAGGGGCTGCGCAAGGCAGCTAGCCTCACGGAG GATCGGGACCGTGGGCGGGATGCCGTGAAGCGAGAAGCTGCCCTACCCCCAGTGAGCCCCCTGAAGGCG GCTCTCTCTGAGGAGGAGTTAGAGAAGAAATCCAAGGCTATCATTGAGGAATATCTCCATCTCAATGAC ATGAAAGAGGCAGTCCAGTGCGTGCAGGAGCTGGCCTCACCCTCCTTGCTCTTCATCTTTGTACGGCAT GGTGTCGAGTCTACGCTGGAGCGCAGTGCCATTGCTCGTGAGCATATGGGGCAGCTGCTGCACCAGCTG CTCTGTGCTGGGCATCTGTCTACTGCTCAGTACTACCAAGGGTTGTATGAAATCTTGGAATTGGCTGAG GACATGGAAATTGACATCCCCCACGTGTGGCTCTACCTAGCGGAACTGGTAACACCCATTCTGCAGGAA GGTGGGGTGCCCATGGGGGAGCTGTTCAGGGAGATTACAAAGCCTCTGAGACCGTTGGGCAAAGCTGCT TCCCTGTTGCTGGAGATCCTGGGCCTCCTGTGCAAAAGCATGGGTCCTAAAAAGGTGGGGACGCTGTGG CGAGAAGCCGGGCTTAGCTGGAAGGAATTTCTACCTGAAGGCCAGGACATTGGTGCATTCGTCGCTGAA CAGAAGGTGGAGTATACCCTGGGAGAGGAGTCGGAAGCCCCTGGCCAGAGGGCACTCCCCTCCGAGGAG CTGAACAGGCAGCTGGAGAAGCTGCTGAAGGAGGGCAGCAGTAACCAGCGGGTGTTCGACTGGATAGAG GCCAACCTGAGTGAGCAGCAGATAGTATCCAACACGTTAGTTCGAGCCCTCATGACGGCTGTCTGCTAT TCTGCAATTATTTTTGAGACTCCCCTCCGAGTGGACGTTGCAGTGCTGAAAGCGCGAGCGAAGCTGCTG CAGAAATACCTGTGTGACGAGCAGAAGGAGCTACAGGCGCTCTACGCCCTCCAGGCCCTTGTAGTGACC TTAGAACAGCCTCCCAACCTGCTGCGGATGTTCTTTGACGCACTGTATGACGAGGACGTGGTGAAGGAG GATGCCTTCTACAGTTGGGAGAGTAGCAAGGACCCCGCTGAGCAGCAGGGCAAGGGTGTGGCCCTTAAA TCTGTCACAGCCTTCTTCAAGTGGCTCCGTGAAGCAGAGGAGGAGTCTGACCACAACTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001194947
Insert Size	4821 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001194947.1
RefSeq Size	5502 bp
RefSeq ORF	4821 bp
Locus ID	1981
Protein Pathways	Viral myocarditis
MW	176.2 kDa
Gene Summary	The protein encoded by this gene is a component of the multi-subunit protein complex EIF4F. This complex facilitates the recruitment of mRNA to the ribosome, which is a rate-limiting step during the initiation phase of protein synthesis. The recognition of the mRNA cap and the ATP-dependent unwinding of 5'-terminal secondary structure is catalyzed by factors in this complex. The subunit encoded by this gene is a large scaffolding protein that contains binding sites for other members of the EIF4F complex. A domain at its N-terminus can also interact with the poly(A)-binding protein, which may mediate the circularization of mRNA during translation. Alternative splicing results in multiple transcript variants, some of which are derived from alternative promoter usage. [provided by RefSeq, Aug 2010] Transcript Variant: This variant (7) differs in the 5' UTR, includes an additional in-frame exon in the 5' coding region, and uses an alternate in-frame splice site in the central coding region, compared to variant 1. The resulting isoform (6) is longer than isoform 1. Both variants 6 and 7 encode the same isoform.

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