REST (NM_001193508) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DFNA27; GINGF5; HGF5; NRSF; WT6; XBR
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331167 representing NM_001193508. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGCCACCCAGGTAATGGGGCAGTCTTCTGGAGGAGGAGGGCTGTTTACCAGCAGTGGCAACATTGGA ATGGCCCTGCCTAACGACATGTATGACTTGCATGACCTTTCCAAAGCTGAACTGGCCGCACCTCAGCTT ATTATGCTGGCAAATGTGGCCTTAACTGGGGAAGTAAATGGCAGCTGCTGTGATTACCTGGTCGGTGAA GAAAGACAGATGGCAGAACTGATGCCGGTTGGGGATAACAACTTTTCAGATAGTGAAGAAGGAGAAGGA CTTGAAGAGTCTGCTGATATAAAAGGTGAACCTCATGGACTGGAAAACATGGAACTGAGAAGTTTGGAA CTCAGCGTCGTAGAACCTCAGCCTGTATTTGAGGCATCAGGTGCTCCAGATATTTACAGTTCAAATAAA GATCTTCCCCCTGAAACACCTGGAGCGGAGGACAAAGGCAAGAGCTCGAAGACCAAACCCTTTCGCTGT AAGCCATGCCAATATGAAGCAGAATCTGAAGAACAGTTTGTGCATCACATCAGAGTTCACAGTGCTAAG AAATTTTTTGTGGAAGAGAGTGCAGAGAAGCAGGCAAAAGCCAGGGAATCTGGCTCTTCCACTGCAGAA GAGGGAGATTTCTCCAAGGGCCCCATTCGCTGTGACCGCTGCGGCTACAATACTAATCGATATGATCAC TATACAGCACACCTGAAACACCACACCAGAGCTGGGGATAATGAGCGAGTCTACAAGTGTATCATTTGC ACATACACAACAGTGAGCGAGTATCACTGGAGGAAACATTTAAGAAACCATTTTCCAAGGAAAGTATAC ACATGTGGAAAATGCAACTATTTTTCAGACAGAAAAAACAATTATGTTCAGCATGTTAGAACTCATACA GGAGAACGCCCATATAAATGTGAACTTTGTCCTTACTCAAGTTCTCAGAAGACTCATCTAACTAGACAT ATGCGTACTCATTCAGGTGAGAAGCCATTTAAATGTGATCAGTGCAGTTATGTGGCCTCTAATCAACAT GAAGTAACCCGCCATGCAAGACAGGTTCACAATGGGCCTAAACCTCTTAATTGCCCACACTGTGATTAC AAAACAGCAGATAGAAGCAACTTCAAAAAACATGTAGAGCTACATGTGAACCCACGGCAGTTCAATTGC CCTGTATGTGACTATGCAGCTTCCAAGAAGTGTAATCTACAGTATCACTTCAAATCTAAGCATCCTACT TGTCCTAATAAAACAATGGATGTCTCAAAAGTGAAACTAAAGAAAACCAAAAAACGAGAGGCTGACTTG CCTGATAATATTACCAATGAAAAAACAGAAATAGAACAAACAAAAATAAAAGGGGATGTGGCTGGAAAG AAAAATGAAAAGTCCGTCAAAGCAGAGAAAAGAGATGTCTCAAAAGAGAAAAAGCCTTCTAATAATGTG TCAGTGATCCAGGTGACTACCAGAACTCGAAAATCAGTAACAGAGGTGAAAGAGATGGATGTGCATACA GGAAGCAATTCAGAAAAATTCAGTAAAACTAAGAAAAGCAAAAGGAAGCTGGAAGTTGACAGCCATTCT TTACATGGTCCTGTGAATGATGAGGAATCTTCAACAAAAAAGAAAAAGAAGGTAGAAAGCAAATCCAAA AATAATAGTCAGGAAGTGCCAAAGGGTGACAGCAAAGTGGAGGAGAATAAAAAGCAAAATACTTGCATG AAAAAAAGTACAAAGAAGAAAACTCTGAAAAATAAATCAAGTAAGAAAAGCAGTAAGCCTCCTCAGAAG GAACCTGTTGAGAAGGGATCTGCTCAGATGGACCCTCCTCAGATGGGGCCTGCTCCCACAGAGGCGGTT CAGAAGGGGCCCGTTCAGGTGGAGCCGCCACCTCCCATGGAGCATGCTCAGATGGAGGGTGCCCAGATA CGGCCTGCTCCTGACGAGCCTGTTCAGATGGAGGTGGTTCAGGAGGGGCCTGCTCAGAAGGAGCTGCTG CCTCCCGTGGAGCCTGCTCAGATGGTGGGTGCCCAAATTGTACTTGCTCACATGGAGCTGCCTCCTCCC ATGGAGACTGCTCAGACGGAGGTTGCCCAAATGGGGCCTGCTCCCATGGAACCTGCTCAGATGGAGGTT GCCCAGGTAGAATCTGCTCCCATGCAGGTGGTCCAGAAGGAGCCTGTTCAGATGGAGCTGTCTCCTCCC ATGGAGGTGGTCCAGAAGGAGCCTGTTCAGATAGAGCTGTCTCCTCCCATGGAGGTGGTCCAGAAGGAA CCTGTTAAGATAGAGCTGTCTCCTCCCATAGAGGTGGTCCAGAAGGAGCCTGTTCAGATGGAGTTGTCT CCTCCCATGGGGGTGGTTCAGAAGGAGCCTGCTCAGAGGGAGCCACCTCCTCCCAGAGAGCCTCCCCTT CACATGGAGCCAATTTCCAAAAAGCCTCCTCTCCGAAAAGATAAAAAGGAAAAGTCTAACATGCAGAGT GAAAGGGCACGGAAGGAGCAAGTCCTTATTGAAGTTGGCTTAGTGCCTGTTAAAGATAGCTGGCTTCTA AAGGAAAGTGTAAGCACAGAGGATCTCTCACCACCATCACCACCACTGCCAAAGGAAAATTTAAGAGAA GAGGCATCAGGAGACCAAAAATTACTCAACACAGGTGAAGGAAATAAAGAAGCCCCTCTTCAGAAAGTA GGAGCAGAAGAGGCAGATGAGAGCCTACCTGGTCTTGCTGCTAATATCAACGAATCTACCCATATTTCA TCCTCTGGACAAAACTTGAATACGCCAGAGGGTGAAACTTTAAATGGTAAACATCAGACTGACAGTATA GTTTGTGAAATGAAAATGGACACTGATCAGAACACAAGAGAGAATCTCACTGGTATAAATTCAACAGTT GAAGAACCAGTTTCACCAATGCTTCCCCCTTCAGCAGTAGAAGAACGTGAAGCAGTGTCCAAAACTGCA CTGGCATCACCTCCTGCTACAATGGCAGCAAATGAGTCTCAGGAAATTGATGAAGATGAAGGCATCCAC AGCCATGAAGGAAGTGACCTAAGTGACAACATGTCAGAGGGTAGTGATGATTCTGGATTGCATGGGGCT CGGCCAGTTCCACAAGAATCTAGCAGAAAAAATGCAAAGGAAGCCTTGGCAGTCAAAGCGGCTAAGGGA GATTTTGTTTGTATCTTCTGTGATCGTTCTTTCAGAAAGGGAAAAGATTACAGCAAACACCTCAATCGC CATTTGGTTAATGTGTACTATCTTGAAGAAGCAGCTCAAGGGCAGGAGTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001193508
Insert Size	3294 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001193508.1
RefSeq Size	7122 bp
RefSeq ORF	3294 bp
Locus ID	5978
UniProt ID	Q13127
Protein Families	Transcription Factors
Protein Pathways	Huntington's disease
MW	121.9 kDa
Gene Summary	This gene was initially identified as a transcriptional repressor that represses neuronal genes in non-neuronal tissues. However, depending on the cellular context, this gene can act as either an oncogene or a tumor suppressor. The encoded protein is a member of the Kruppel-type zinc finger transcription factor family. It represses transcription by binding a DNA sequence element called the neuron-restrictive silencer element. The protein is also found in undifferentiated neuronal progenitor cells and it is thought that this repressor may act as a master negative regulator of neurogenesis. Alternatively spliced transcript variants have been described. [provided by RefSeq, May 2018] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein.

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