Claudin 15 (CLDN15) (NM_001185080) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331133 representing NM_001185080. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGTCGATGGCTGTGGAAACCTTTGGCTTCTTCATGGCAACTGTGGGGCTGCTGATGCTGGGGGTGACT CTGCCAAACAGCTACTGGCGAGTGTCCACTGTGCACGGGAACGTCATCACCACCAACACCATCTTCGAG AACCTCTGGTTTAGCTGTGCCACCGACTCCCTGGGCGTCTACAACTGCTGGGAGTTCCCGTCCATGCTG GCCCTCTCTGGGTATATTCAGGCCTGCCGGGCACTCATGATCACCGCCATCCTCCTGGGCTTCCTCGGC CTCTTGCTAGGCATAGCGGGCCTGCGCTGCACCAACATTGGGGGCCTGGAGCTCTCCAGGAAAGCCAAG CTGGCGGCCACCGCAGGGGCCCTCCACATTCTGGCCGGTATCTGCGGGATGGTGGCCATCTCCTGGTAC GCCTTCAACATCACCCGGGACTTCTTCGACCCCTTGTACCCCGGAACCAAGTACGAGCTGGGCCCCGCC CTCTACCTGGGGTGGAGCGCCTCACTGATCTCCATCCTGGGTGGCCTCTGCCTCTGCTCCGCCTGCTGC TGCGGCTCTGACGAGGACCCAGCCGCCAGCGCCCGGCGGCCCTACCAGGCTCCAGTGTCCGTGATGCCC GTCGCCACCTCGGACCAAGAAGGCGACAGCAGCTTTGGCAAATACGGCAGAAACGCCTACGTGTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001185080
Insert Size	687 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001185080.1
RefSeq Size	2145 bp
RefSeq ORF	687 bp
Locus ID	24146
UniProt ID	P56746
Protein Families	Transmembrane
Protein Pathways	Cell adhesion molecules (CAMs), Leukocyte transendothelial migration, Tight junction
MW	24.4 kDa
Gene Summary	This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jun 2010] Transcript Variant: This variant (1) is the longer transcript. Variants 1 and 2 encode the same protein.

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