NR5A2 (NM_001276464) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	B1F; B1F2; CPF; FTF; FTZ-F1; FTZ-F1beta; hB1F-2; LRH-1; LRH1
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331062 representing NM_001276464. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGTGAATTACTCCTATGATGAAGATCTGGAAGAGCTTTGTCCCGTGTGTGGAGATAAAGTGTCTGGG TACCATTATGGGCTCCTCACCTGTGAAAGCTGCAAGGGATTTTTTAAGCGAACAGTCCAAAATAATAAA AGGTACACATGTATAGAAAACCAGAACTGCCAAATTGACAAAACACAGAGAAAGCGTTGTCCTTACTGT CGTTTTCAAAAATGTCTAAGTGTTGGAATGAAGCTAGAAGCTGTAAGGGCCGACCGAATGCGTGGAGGA AGGAATAAGTTTGGGCCAATGTACAAGAGAGACAGGGCCCTGAAGCAACAGAAAAAAGCCCTCATCCGA GCCAATGGACTTAAGCTAGAAGCCATGTCTCAGGTGATCCAAGCTATGCCCTCTGACCTGACCATTTCC TCTGCAATTCAAAACATCCACTCTGCCTCCAAAGGCCTACCTCTGAACCATGCTGCCTTGCCTCCTACA GACTATGACAGAAGTCCCTTTGTAACATCCCCCATTAGCATGACAATGCCCCCTCACGGCAGCCTGCAA GGTTACCAAACATATGGCCACTTTCCTAGCCGGGCCATCAAGTCTGAGTACCCAGACCCCTATACCAGC TCACCCGAGTCCATAATGGGCTATTCATATATGGATAGTTACCAGACGAGCTCTCCAGCAAGCATCCCA CATCTGATACTGGAACTTTTGAAGTGTGAGCCAGATGAGCCTCAAGTCCAGGCTAAAATCATGGCCTAT TTGCAGCAAGAGCAGGCTAACCGAAGCAAGCACGAAAAGCTGAGCACCTTTGGGCTTATGTGCAAAATG GCAGATCAAACTCTCTTCTCCATTGTCGAGTGGGCCAGGAGTAGTATCTTCTTCAGAGAACTTAAGGTT GATGACCAAATGAAGCTGCTTCAGAACTGCTGGAGTGAGCTCTTAATCCTCGACCACATTTACCGACAA GTGGTACATGGAAAGGAAGGATCCATCTTCCTGGTTACTGGGCAACAAGTGGACTATTCCATAATAGCA TCACAAGCCGGAGCCACCCTCAACAACCTCATGAGTCATGCACAGGAGTTAGTGGCAAAACTTCGTTCT CTCCAGTTTGATCAACGAGAGTTCGTATGTCTGAAATTCTTGGTGCTCTTTAGTTTAGATGTCAAAAAC CTTGAAAACTTCCAGCTGGTAGAAGGTGTCCAGGAACAAGTCAATGCCGCCCTGCTGGACTACACAATG TGTAACTACCCGCAGCAGACAGAGAAATTTGGACAGCTACTTCTTCGACTACCCGAAATCCGGGCCATC AGTATGCAGGCTGAAGAATACCTCTACTACAAGCACCTGAACGGGGATGTGCCCTATAATAACCTTCTC ATTGAAATGTTGCATGCCAAAAGAGCATAA
Restriction Sites	SgfI-MluI
ACCN	NM_001276464
Insert Size	1410 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001276464.1
RefSeq Size	4787 bp
RefSeq ORF	1410 bp
Locus ID	2494
UniProt ID	O00482
Protein Families	Druggable Genome, Nuclear Hormone Receptor, Transcription Factors
Protein Pathways	Maturity onset diabetes of the young
MW	53.7 kDa
Gene Summary	The protein encoded by this gene is a DNA-binding zinc finger transcription factor and is a member of the fushi tarazu factor-1 subfamily of orphan nuclear receptors. The encoded protein is involved in the expression of genes for hepatitis B virus and cholesterol biosynthesis, and may be an important regulator of embryonic development. [provided by RefSeq, Jun 2016] Transcript Variant: This variant (3) has an alternate exon in place of the first two exons compared to variant 1. The resulting isoform (3) uses a downstream translation start site and has a shorter N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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