ELF2 (NM_001276457) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	b; EU32; NERF; NERF-1A; NERF-1a; NERF-1B; NERF-2
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331060 representing NM_001276457. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGCGACGTCTCTGCATGAGGGACCCACGAACCAGCTGGATCTGCTCATCCGGGCCGTGGAAGCATCA GTTCACAGCAGTAATGCACACTGTACAGATAAGACAATTGAAGCTGCTGAAGCCCTGCTTCATATGGAA TCTCCTACCTGCTTGAGGGATTCAAGAAGTCCTGTGGAAGTGTTTGTTCCTCCTTGTGTATCAACTCCA GAATTCATCCATGCTGCTATGAGGCCAGATGTCATTACAGAAACTGTAGTGGAGGTGTCAACTGAAGAG TCTGAACCCATGGATACCTCTCCTATTCCAACATCACCAGATAGCCATGAACCAATGAAAAAGAAAAAA GGAAACACAACCTATTTGTGGGAGTTTCTTTTAGATCTACTTCAAGATAAAAATACTTGTCCCAGGTAT ATTAAATGGACTCAGAGAGAAAAAGGCATATTCAAGCTGGTGGATTCAAAGGCTGTCTCTAAGCTTTGG GGAAAGCATAAGAACAAACCAGACATGAACTATGAAACCATGGGACGAGCTTTGAGATACTACTACCAA AGGGGAATTCTTGCAAAGGTTGAAGGACAGAGGCTTGTATATCAGTTCAAGGATATGCCGAAAAACATA GTGGTCATAGATGATGACAAAAGTGAAACCTGTAATGAAGATTTAGCAGGAACTACTGATGAAAAATCA TTAGAACGAGTGTCACTGTCTGCAGAAAGTCTCCTGAAAGCAGCATCCTCTGTTCGCAGTGGAAAAAAT TCATCCCCTATAAACTGCTCCAGAGCAGAGAAGGGTGTAGCTAGAGTTGTGAATATCACTTCCCCTGGG CACGATGCTTCATCCAGGTCTCCTACTACCACTGCATCTGTGTCAGCAACAGCAGCTCCAAGGACAGTT CGTGTGGCAATGCAGGTACCTGTTGTAATGACATCATTGGGTCAGAAAATTTCAACTGTGGCAGTTCAG TCAGTTAATGCAGGTGCACCATTAATAACCAGCACTAGTCCAACAACAGCGACCTCTCCAAAGGTAGTC ATTCAGACAATCCCTACTGTGATGCCAGCTTCTACTGAAAATGGAGACAAAATCACCATGCAGCCTGCC AAAATTATTACCATCCCAGCTACACAGCTTGCACAGTGTCAACTGCAGACAAAGTCAAATCTGACTGGA TCAGGAAGCATTAACATTGTTGGAACCCCATTGGCTGTGAGAGCACTTACCCCTGTTTCAATAGCCCAT GGTACACCTGTAATGAGACTATCAATGCCTACTCAGCAGGCATCTGGCCAGACTCCTCCTCGAGTTATC AGTGCAGTCATAAAGGGGCCAGAGGTTAAATCGGAAGCAGTGGCAAAAAAGCAAGAACATGATGTGAAA ACTTTGCAGCTAGTAGAAGAAAAACCAGCAGATGGAAATAAGACAGTGACCCACGTAGTGGTTGTCAGT GCGCCTTCAGCTATTGCCCTTCCTGTAACTATGAAAACAGAAGGACTAGTGACATGTGAGAAATAA
Restriction Sites	SgfI-MluI
ACCN	NM_001276457
Insert Size	1515 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001276457.1
RefSeq Size	3005 bp
RefSeq ORF	1515 bp
Locus ID	1998
UniProt ID	Q15723
Protein Families	Druggable Genome, Transcription Factors
MW	54.3 kDa
Gene Summary	Isoform 1 transcriptionally activates the LYN and BLK promoters and acts synergistically with RUNX1 to transactivate the BLK promoter.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) has an alternate exon in place of the first three exons, uses an alternate in-frame splice junction at the 5' end of an internal exon, and lacks an alternate in-frame exon compared to variant 1. Translation starts in the alternate exon, so the resulting isoform (3) has a shorter and distinct N-terminus, contains an alternate internal segment, and lacks an alternate internal segment compared to isoform 1.

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