PPP2R2B (NM_001271948) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	B55BETA; PP2AB55BETA; PP2ABBETA; PP2APR55B; PP2APR55BETA; PR2AB55BETA; PR2ABBETA; PR2APR55BETA; PR52B; PR55-BETA; PR55BETA; SCA12
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331012 representing NM_001271948. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGAATTATCCAGATGAAAACACCTATGGAAATAAAGCTGACATTATCTCTACGGTAGAATTCAACCAC ACGGGAGAATTACTAGCGACAGGGGACAAGGGGGGTCGGGTTGTAATATTTCAACGAGAGCAGGAGAGT AAAAATCAGGTTCATCGTAGGGGTGAATACAATGTTTACAGCACATTCCAGAGCCATGAACCCGAGTTC GATTACCTGAAGAGTTTAGAAATAGAAGAAAAAATCAATAAAATAAGATGGCTCCCCCAGCAGAATGCA GCTTACTTTCTTCTGTCTACTAATGATAAAACTGTGAAGCTGTGGAAAGTCAGCGAGCGTGATAAGAGG CCAGAAGGCTACAATCTGAAAGATGAGGAGGGCCGGCTCCGGGATCCTGCCACCATCACAACCCTGCGG GTGCCTGTCCTGAGACCCATGGACCTGATGGTGGAGGCCACCCCACGAAGAGTATTTGCCAACGCACAC ACATATCACATCAACTCCATATCTGTCAACAGCGACTATGAAACCTACATGTCCGCTGATGACCTGAGG ATTAACCTATGGAACTTTGAAATAACCAATCAAAGTTTTAATATTGTGGACATTAAGCCAGCCAACATG GAGGAGCTCACGGAGGTGATCACAGCAGCCGAGTTCCACCCCCATCATTGCAACACCTTCGTGTACAGC AGCAGCAAAGGGACAATCCGGCTGTGTGACATGCGGGCATCTGCCCTGTGTGACAGGCACACCAAATTT TTTGAAGAGCCGGAAGATCCAAGCAACAGATCATTTTTCTCTGAAATTATCTCTTCGATTTCGGATGTG AAGTTCAGCCACAGTGGGAGGTATATCATGACCAGGGACTACTTGACCGTCAAAGTCTGGGATCTCAAC ATGGAAAACCGCCCCATCGAGACTTACCAGGTTCATGACTACCTCCGCAGCAAGCTGTGTTCCCTCTAT GAAAATGACTGCATTTTTGATAAATTTGAGTGTGTGTGGAATGGGTCAGACAGTGTCATCATGACAGGC TCCTACAACAACTTCTTCAGGATGTTCGACAGAAACACCAAGCGTGATGTGACCCTTGAGGCTTCGAGG GAAAACAGCAAGCCCCGGGCTATCCTCAAACCCCGAAAAGTGTGTGTGGGGGGCAAGCGGAGAAAAGAC GAGATCAGTGTCGACAGTCTGGACTTTAGCAAAAAGATCTTGCATACAGCTTGGCATCCTTCAGAAAAT ATTATAGCAGTGGCGGCTACAAATAACCTATATATATTCCAGGACAAGGTTAACTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001271948
Insert Size	1299 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001271948.1
RefSeq Size	2578 bp
RefSeq ORF	1299 bp
Locus ID	5521
UniProt ID	Q00005
Protein Families	Druggable Genome, Phosphatase
Protein Pathways	Tight junction
MW	50.4 kDa
Gene Summary	The product of this gene belongs to the phosphatase 2 regulatory subunit B family. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes a beta isoform of the regulatory subunit B55 subfamily. Defects in this gene cause autosomal dominant spinocerebellar ataxia 12 (SCA12), a disease caused by degeneration of the cerebellum, sometimes involving the brainstem and spinal cord, and in resulting in poor coordination of speech and body movements. Multiple alternatively spliced variants, which encode different isoforms, have been identified for this gene. The 5' UTR of some of these variants includes a CAG trinucleotide repeat sequence (7-28 copies) that can be expanded to 55-78 copies in cases of SCA12. [provided by RefSeq, Jul 2016] Transcript Variant: This variant (10) has an additional segment and an additional exon in the 5' region, and initiates translation at an alternate downstream start codon, compared to variant 3. The resulting isoform (d) is shorter and has a distinct N-terminus, compared to isoform e. Variants 6 and 10 encode the same isoform d.

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