AAGAB (NM_001271886) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	KPPP1; p34; PPKP1; PPKP1A
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC330994 representing NM_001271886. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGATCTTGGTCTGCGATAGAGTGTCTGAAGATGGTATAAACCGACAAAAAGCTCAAGAATGGTGCATC AAACATGGCTTTGAATTGGTAGAACTTAGTCCAGAGGAGTTGCCTGAGGAGGATGATGACTTCCCAGAA TCTACAGGAGTAAAGCGAATTGTCCAAGCCCTGAATGCCAATGTGTGGTCCAATGTAGTGATGAAGAAT GATAGGAACCAAGGCTTTAGCCTTCTCAACTCATTGACTGGAACAAACCATAGCATTGGGTCAGCAGAT CCCTGTCACCCAGAGCAACCCCATTTGCCAGCAGCAGATAGTACTGAATCCCTCTCTGATCATCGGGGT GGTGCATCTAACACAACAGATGCCCAGGTTGATAGCATTGTGGATCCCATGTTAGATCTGGATATTCAA GAATTAGCCAGTCTTACCACTGGAGGAGGAGATGTGGAGAATTTTGAAAGACTCTTTTCAAAGTTAAAG GAAATGAAAGACAAGGCTGCGACGCTTCCTCATGAGCAAAGAAAAGTGCATGCAGAAAAGGTGGCCAAA GCATTCTGGATGGCAATCGGGGGAGACAGAGATGAAATTGAAGGCCTTTCATCTGATGAAGAGCACTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001271886
Insert Size	621 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001271886.1
RefSeq Size	3344 bp
RefSeq ORF	621 bp
Locus ID	79719
UniProt ID	Q6PD74
MW	22.7 kDa
Gene Summary	The protein encoded by this gene interacts with the gamma-adaptin and alpha-adaptin subunits of complexes involved in clathrin-coated vesicle trafficking. Mutations in this gene are associated with type I punctate palmoplantar keratoderma. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2012] Transcript Variant: This variant (3) contains an alternatively spliced 5' terminal exon, which causes translation initiation from an in-frame downstream start codon compared to variant 1. The resulting isoform (2) has a shorter N-terminus compared to isoform 1. Variants 2 and 3 encode the same isoform.

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