PSMD5 (NM_001270427) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	S5B
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC330831 representing NM_001270427. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGCAGCCCAGGCTTTGGCGCTGCTGAGAGAGGTAGCGAGGCTGGAAGCGCCGCTGGAGGAGCTACGC GCGCTTCACTCCGTGCTGCAGGCAGTGCCGCTCAACGAGCTTCGCCAGCAAGCGGCGGAGCTGCGCCTC GGCCCGCTCTTCTCCCTGCTTAACGAGAACCATAGGGAAAAGACTACTTTGTGTGTATCCATTCTGGAG AGATTGCTCCAAGCTATGGAACCGGTTCACGTGGCCCGGAACCTCAGGGTTGACCTGCAGAGGGGACTA ATTCACCCTGATGATTCTGTAAAAATCCTCACTCTTTCCCAGATTGGAAGAATTGTTGAAAATTCTGAT GCTGTTACTGAGATTCTAAATAATGCTGAATTACTAAAACAAATTGTTTATTGCATTGGTGGAGAGAAT CTATCTGTAGCAAAAGCGCTAATTATAGAGATTTCTTCCGTGTCACCAGAATCTTTAAACTACTGTACC ACAAGTGGATTGGTAACCCAGCTCCTGAGAGAGCTGACTGGTGAGGATGTGTTGGTCAGAGCCACCTGT ATAGAAATGGTGACATCACTGGCATATACTCATCATGGGCGACAATATCTTGCTCAAGAAGGAGTAATT GACCAAATTTCTAATATAATTGTTGGGGCAGATTCAGACCCTTTCTCTAGCTTCTATCTGCCAGGATTC GTGAAGTTTTTTGGAAACCTGGCTGTCATGGATAGTCCTCAACAGATCTGTGAGCGTTATCCTATCTTT GTGGAAAAAGTCTTTGAAATGATAGAAAGTCAGGACCCCACTATGATTGGTGTAGCTGTAGACACAGTT GGAATCTTGGGATCCAATGTTGAAGGAAAACAGGTTTTACAGAAAACAGGAACTCGCTTTGAACGCTTG CTTATGAGAATAGGACATCAATCAAAGAATGCCCCAGTGGAGCTAAAAATTAGATGTTTGGATGCAATT TCATCTCTTCTGTACTTACCACCTGAGCAGCAGACTGATGACCTTCTGAGGATGACAGAATCCTGGTTT TCTTCTTTATCTCGGGATCCACTGGAGCTCTTCCGTGGCATTAGTAGTCAGCCCTTCCCTGAACTACAC TGTGCTGCCTTAAAAGTGTTTACGGCCATTGCAAACCAACCCTGGGCTCAGAAACTTATGTTTAACAGT CCAGGTTTTGTAGAATATGTGGTGGACCGGTCTGTGGAGCATGACAAAGCTTCAAAGGATGCCAAATAT GAACTAGTGAAAGCACTTGCCAATTCCAAGACAATTGCAGAAATCTTTGGGAACCCAAATTATTTGAGG CTCAGAACTTACCTGAGTGAAGGGCCATACTATGTGAAACCTGTTTCCACGACAGCAGTAGAAGGAGCC GAATGA
Restriction Sites	SgfI-MluI
ACCN	NM_001270427
Insert Size	1386 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001270427.1
RefSeq Size	3375 bp
RefSeq ORF	1386 bp
Locus ID	5711
UniProt ID	Q16401
MW	51.3 kDa
Gene Summary	The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. This gene encodes a non-ATPase subunit of the 19S regulator base that functions as a chaperone protein during 26S proteasome assembly. [provided by RefSeq, Jul 2012] Transcript Variant: This variant (2) lacks an exon in the coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (2) is shorter than isoform 1.

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