UCK (UCK1) (NM_001261451) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	URK1
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC330717 representing NM_001261451. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGCTTCGGCGGGAGGCGAAGACTGCGAGAGCCCCGCGCCGGAGGCCGACCGTCCGCACCAGCGGCCC TTCCTGATAGGGGACGAGCGGTTCCAGGCGGGAATCCCGCTTCTGTGTCTCCAGTCGACCGTGTGTGAG AAGATCATGGAGTTGCTGGGACAGAACGAGGTGGAACAGCGGCAGCGGAAGGTGGTCATCCTGAGCCAG GACAGGTTCTACAAGGTCCTGACGGCAGAGCAGAAGGCCAAGGCCTTGAAAGGACAGTACAATTTTGAC CATCCAGATGCCTTTGATAATGATTTGATGCACAGGACTCTGAAGAACATCGTGGAGGGCAAAACGGTG GAGGTGCCGACCTATGATTTTGTGACACACTCAAGGTTACCAGAGACCACGGTGGTCTACCCTGCGGAC GTGGTTCTGTTTGAGGGCATCTTGGTGTTCTACAGCCAGGAGATCCGGGACATGTTCCACCTGCGCCTC TTCGTGGACACCGACTCCGACGTCAGGCTGTCTCGAAGAGTTCTCCGGGACGTGCGCCGAGGGAGGGAC CTGGAGCAGATTCTGACGCAGTACACCACCTTCGTGAAGCCGGCCTTCGAGGAGTTCTGCCTGCCGACA AAGAAGTATGCCGATGTGATCATCCCGCGAGGAGTGGACAATATGGTTGCCATCAACCTGATCGTGCAG CACATCCAGGACATTCTGAATGGTGACATCTGCAAATGGCACCGAGGAGGGTCCAATGGGCGGAGCTAC AAGCGGACCTTTTCTGAGCCAGGGGACCACCCTGGGATGCTGACCTCTGGCAAACGGTCACATTTGGAG TCCAGCAGCAGACCCCACTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001261451
Insert Size	849 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001261451.1
RefSeq Size	2206 bp
RefSeq ORF	849 bp
Locus ID	83549
UniProt ID	Q9HA47
Protein Families	Druggable Genome
Protein Pathways	Drug metabolism - other enzymes, Metabolic pathways, Pyrimidine metabolism
MW	32.3 kDa
Gene Summary	This gene encodes a uridine-cytidine kinase that catalyzes the phosphorylation of uridine and cytidine to uridine monophosphate (UMP) and cytidine monophosphate (CMP) but not the phosphorylation of deoxyribonucleosides or purine ribonucleosides. This enzyme can also phosphorylate uridine and cytidine analogs and uses both ATP and GTP as a phosphate donor. Alternative splicing results in multiple splice variants encoding distinct isoforms. [provided by RefSeq, May 2012] Transcript Variant: This variant (4) has multiple differences in the 5' coding region, compared to variant 1, that result in a protein (isoform d) with a longer N-terminus, compared to isoform a.

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