Dynamitin (DCTN2) (NM_001261413) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DCTN50; DYNAMITIN; HEL-S-77; RBP50
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC330705 representing NM_001261413. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGCGGACCCTAAATACGCCGACCTTCCCGGCATTGCCAGGAATGAGCCAGATGTTTATGAAACTAGC GACCTACCTGAGGATGATCAAGCGGAGTTCGATGCGGAGGAGCTGACAAGCACAAGTGTGGAACACATC ATTGTCAATCCTAATGCTGCCTATGACAAGTTCAAGGACAAGAGAGTGGGGACAAAGGGACTTGATTTC TCAGATCGTATTGGAAAAACCAAGAGGACAGGATATGAATCTGGAGAATATGAGATGCTTGGAGAGGGT CTGGGAGTGAAGGAGACACCCCAGCAAAAGTACCAGCGCCTACTGCATGAGGTCCAAGAGCTGACAACT GAAGTTGAAAAAATCAAGACGACAGTGAAGGAGTCAGCCACAGAGGAGAAGCTGACCCCTGTGTTGCTG GCTAAACAGCTGGCAGCCCTGAAGCAGCAGCTGGTTGCTTCCCACCTGGAGAAGCTGCTGGGACCAGAT GCTGCAATCAACCTTACCGACCCCGATGGCGCCCTGGCTAAGCGCCTACTACTGCAGCTGGAAGCAACA AAGAACAGCAAAGGGGGATCAGGGGGAAAAACCACTGGGACCCCCCCAGATAGCAGCCTTGTCACTTAT GAACTACATTCTCGGCCTGAGCAGGACAAGTTCTCTCAAGCTGCCAAAGTCGCAGAACTTGAAAAGCGC CTGACAGAGCTGGAGACAGCTGTACGTTGTGATCAGGATGCTCAGAATCCCCTTTCTGCAGGTCTACAG GGAGCCTGTCTCATGGAGACTGTAGAGCTGTTGCAAGCAAAGGTGAGCGCCCTAGACCTTGCAGTTTTG GATCAAGTGGAGGCTCGGCTACAGAGTGTCCTGGGAAAGGTGAACGAGATTGCCAAGCATAAAGCCTCT GTAGAAGATGCAGATACACAAAGCAAGGTGCACCAGCTATATGAAACTATACAGCGCTGGAGCCCCATT GCCTCCACCCTCCCTGAGCTGGTGCAGAGACTTGTCACCATCAAGCAGCTGCACGAGCAAGCCATGCAG TTTGGTCAGCTCCTGACACACTTGGATACCACCCAGCAGATGATTGCTAATTCCTTGAAGGACAATACC ACCCTCTTGACCCAGGTGCAGACAACCATGCGTGAAAACCTGGCCACAGTTGAGGGGAACTTTGCCAGC ATTGATGAACGGATGAAGAAGCTGGGAAAGTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001261413
Insert Size	1206 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001261413.1
RefSeq Size	2113 bp
RefSeq ORF	1206 bp
Locus ID	10540
UniProt ID	Q13561
Protein Pathways	Huntington's disease
MW	44.2 kDa
Gene Summary	This gene encodes a 50-kD subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit is present in 4-5 copies per dynactin molecule. It contains three short alpha-helical coiled-coil domains that may mediate association with self or other dynactin subunits. It may interact directly with the largest subunit (p150) of dynactin and may affix p150 in place. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2012] Transcript Variant: This variant (3) lacks two exons in the 5' coding region but maintains the reading frame, compared to variant 1. The resulting isoform (3) lacks an internal segment, compared to isoform 1.

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