Apc10 (ANAPC10) (NM_001256709) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	APC10; DOC1
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC330505 representing NM_001256709. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGGAGGTGTCAAGGAAACGAATATTTTAAAAATGACTACACCAAACAAGACACCTCCTGGTGCTGAC CCCAAGCAGTTGGAAAGGACTGGAACAGTACGGGAAATTGGGTCACAAGCTGTTTGGTCACTCTCATCT TGCAAACCAGGATTTGGAGTGGATCAGTTACGAGATGACAATCTAGAAACTTATTGGCAATCAGATGGT TCCCAGCCTCATTTAGTGAACATCCAATTCAGAAGAAAAACAACAGTGAAGACATTATGTATTTATGCA GACTACAAATCTGATGAAAGCTATACTCCAAGCAAGATCTCAGTCAGAGTAGGAAATAATTTTCACAAC CTTCAAGAAATTCGGCAACTTGAGTTGGTGGAACCAAGTGGCTGGATTCATGTTCCCTTAACTGACAAT CATAAGAAGCCAACTCGTACATTCATGATACAGATTGCTGTTCTAGCCAATCACCAGAATGGAAGAGAC ACCCATATGAGACAAATTAAAATATACACACCAGTAGAAGAGAGCTCCATTGGTAAATTTCCTAGATGT ACAACTATAGATTTCATGATGTATCGTTCAATAAGGTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001256709
Insert Size	591 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001256709.1
RefSeq Size	1609 bp
RefSeq ORF	591 bp
Locus ID	10393
UniProt ID	Q9UM13
Protein Families	Druggable Genome
Protein Pathways	Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation, Ubiquitin mediated proteolysis
MW	22.4 kDa
Gene Summary	ANAPC10 is a core subunit of the anaphase-promoting complex (APC), or cyclosome, a ubiquitin protein ligase that is essential for progression through the cell cycle. APC initiates sister chromatid separation by ubiquitinating the anaphase inhibitor securin (PTTG1; MIM 604147) and triggers exit from mitosis by ubiquitinating cyclin B (CCNB1; MIM 123836), the activating subunit of cyclin-dependent kinase-1 (CDK1; MIM 116940) (summary by Wendt et al., 2001 [PubMed 11524682]).[supplied by OMIM, Feb 2011] Transcript Variant: This variant (5) differs in the 5' UTR and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) is longer and has a distinct N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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