ACSS1 (NM_001252676) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ACAS2L; ACECS1; AceCS2L
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC330294 representing NM_001252676. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGAGGCGCCGAAAGGAAAGACAACCTTGGGATCGCTTCCACTTCCTCCACTTCGCACCGCATGGCCGG GAACTACTGGAGACCACGTGCCGCCTGGCCAACACGCTGAAGAGGCATGGAGTCCACCGTGGGGACCGT GTTGCCATCTACATGCCCGTGTCCCCATTGGCTGTGGCAGCAATGCTGGCCTGTGCCAGGATCGGAGCT GTCCACACAGTCATCTTTGCTGGCTTCAGTGCAGAGTCCTTGGCTGGGAGGATCAATGATGCCAAGTGC AAGGTGGTTATCACCTTCAACCAAGGACTCCGGGGTGGGCGCGTGGTGGAGCTGAAGAAAATAGTGGAT GAGGCTGTGAAGCACTGCCCCACCGTGCAGCATGTCCTGGTGGCTCACAGGACAGACAACAAGGTCCAC ATGGGGGATCTGGACGTCCCGCTGGAGCAGGAAATGGCCAAGGAGGACCCTGTTTGCGCCCCAGAGAGC ATGGGCAGTGAGGACATGCTCTTCATGCTGTACACCTCAGGGAGCACCGGAATGCCCAAGGGCATCGTC CATACCCAGGCAGGCTACCTGCTCTATGCCGCCCTGACTCACAAGCTTGTGTTTGACCACCAGCCAGGT GACATCTTTGGCTGTGTGGCCGACATCGGTTGGATTACAGGACACAGCTACGTGGTGTATGGGCCTCTC TGCAATGGTGCCACCAGCGTCCTTTTTGAGAGCACCCCAGTTTATCCCAATGCTGGTCGGTACTGGGAG ACAGTAGAGAGGTTGAAGATCAATCAGTTCTATGGCGCCCCAACGGCTGTCCGGCTGTTGCTGAAATAC GGTGATGCCTGGGTGAAGAAGTATGATCGCTCCTCCCTGCGGACCCTGGGGTCAGTGGGAGAGCCCATC AACTGTGAGGCCTGGGAGTGGCTTCACAGGGTGGTGGGGGACAGCAGGTGCACGCTGGTGGACACCTGG TGGCAGACAGAAACAGGTGGCATCTGCATCGCACCACGGCCCTCGGAAGAAGGGGCGGAAATCCTCCCT GCCATGGCGATGAGGCCCTTCTTTGGCATCGTCCCCGTCCTCATGGATGAGAAGGGCAGCGTCGTGGAG GGCAGCAACGTCTCCGGGGCCCTGTGCATCTCCCAGGCCTGGCCGGGCATGGCCAGGACCATCTATGGC GACCACCAGCGATTTGTGGACGCCTACTTCAAGGCCTACCCAGGCTATTACTTCACTGGAGACGGGGCT TACCGAACTGAGGGCGGCTATTACCAGATCACAGGGCGGATGGATGATGTCATCAACATCAGTGGCCAC CGGCTGGGGACCGCAGAGATTGAGGACGCCATCGCCGACCACCCTGCAGTACCAGAAAGTGCTGTCATT GGCTACCCCCACGACATCAAAGGAGAAGCTGCCTTTGCCTTCATTGTGGTGAAAGATAGTGCGGGTGAC TCAGATGTGGTGGTGCAGGAGCTCAAGTCCATGGTGGCCACCAAGATCGCCAAATATGCTGTGCCTGAT GAGATCCTGGTGGTGAAACGTCTTCCAAAAACCAGGTCTGGGAAGGTCATGCGGCGGCTCCTGAGGAAG ATCATCACTAGTGAGGCCCAGGAGCTGGGAGACACTACCACCTTGGAGGACCCCAGCATCATCGCAGAG ATCCTGAGTGTCTACCAGAAGTGCAAGGACAAGCAGGCTGCTGCTAAGTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001252676
Insert Size	1707 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001252676.1
RefSeq Size	3519 bp
RefSeq ORF	1707 bp
Locus ID	84532
UniProt ID	Q9NUB1
Protein Pathways	Glycolysis / Gluconeogenesis, Metabolic pathways, Propanoate metabolism, Pyruvate metabolism
MW	62.7 kDa
Gene Summary	This gene encodes a mitochondrial acetyl-CoA synthetase enzyme. A similar protein in mice plays an important role in the tricarboxylic acid cycle by catalyzing the conversion of acetate to acetyl CoA. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011] Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (3) is shorter and has a distinct N-terminus, compared to isoform 1.

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