AP4E1 (NM_001252127) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CPSQ4; SPG51; STUT1
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC330273 representing NM_001252127. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGATGAAGGAATGTATGGTGAGACTTATATATTGTGAAATGCTTGGATATGATGCTTCCTTTGGCTAT ATTCATGCAATCAAGTTAGCCCAACAAGGAAACCTCTTAGAAAAAAGAGTAGGTTATTTGGCTGTTTCC TTATTTCTACATGAAAGTCATGAATTATTGCTTCTCCTTGTGAATACAGTTGTAAAGGATCTGCAGAGC ACTAACCTAGTAGAAGTGTGTATGGCACTGACTGTTGTTAGCCAGATTTTCCCCTGCGAAATGATTCCA GCTGTTCTTCCATTAATAGAAGATAAACTTCAACATTCTAAGGAGATTGTACGAAGAAAAGCTGTTCTG GCATTATACAAATTCCATCTCATTGCTCCTAATCAAGTACAACATATTCATATTAAGTTTCGGAAAGCA CTTTGTGACAGAGATGTTGGGGTCATGGCTGCCTCCTTGCATATATATCTTAGAATGATTAAGGAGAAT TCATCTGGATATAAAGACTTGACTGGGAGTTTTGTAACCATTTTGAAGCAAGTAGTTGGAGGAAAGCTC CCAGTAGAATTCAATTACCACAGTGTGCCAGCACCATGGTTACAAATTCAGCTCTTGAGAATACTGGGA CTTCTAGGAAAAGATGATCAAAGGACAAGTGAATTAATGTATGATGTTCTTGATGAATCCTTACGAAGA GCTGAGTTAAATCACAATGTCACATATGCTATTTTGTTTGAATGTGTGCATACAGTCTATTCTATTTAT CCTAAATCGGAATTACTTGAGAAGGCTGCCAAGTGCATTGGAAAATTTGTTCTGTCACCTAAAATAAAT CTAAAATATTTAGGACTGAAGGCTCTTACCTATGTTATCCAACAGGATCCTACTCTGGCTCTTCAACAC CAGATGACAATAATTGAATGTTTAGATCATCCTGATCCCATTATTAAAAGAGAGACTCTGGAACTTCTT TACAGAATTACTAATGCACAGAATATAACAGTTATTGTCCAGAAAATGCTTGAATATTTACATCAGAGC AAAGAAGAGTATGTCATCGTCAATTTGGTCGGCAAAATAGCAGAGCTGGCTGAGAAATATGCTCCTGAT AATGCATGGTTTATTCAGACAATGAATGCTGTGTTTTCAGTAGGAGGAGATGTAATGCATCCTGATATT CCCAATAACTTTCTGAGACTACTAGCGGAAGGTTTTGATGATGAAACAGAAGATCAGCAATTAAGACTC TATGCAGTTCAGTCTTATCTCACTTTACTGGATATGGAAAATGTGTTCTATCCACAGAGATTTCTTCAA GTTATGAGTTGGGTATTAGGGGAATATTCCTACCTCTTAGATAAGGAAACGCCAGAGGAAGTTATAGCT AAGCTCTACAAGTTACTTATGAATGACTCTGTGTCTTCAGAAACAAAAGCCTGGTTAATTGCTGCTGTG ACCAAATTGACATCTCAGGCGCACTCTTCTAATACAGTTGAGAGATTAATCCATGAATTTACCATATCT TTGGATACTTGTATGAGACAACATGCATTTGAATTAAAACATTTGCATGAGAATGTGGAACTTATGAAG AGCTTGCTTCCAGTTGACAGGAGTTGTGAAGACTTGGTGGTAGATGCTTCTTTATCTTTTCTGGATGGT TTTGTGGCTGAAGGACTCAGTCAGGGTGCAGCGCCTTACAAACCTCCCCATCAACGCCAGGAGGAAAAG CTTTCTCAGGAAAAAGTTCTCAATTTTGAACCATATGGACTCTCCTTTTCTTCATCTGGCTTCACTGGA CGACAGTCTCCTGCTGGCATTTCTCTTGGTTCAGATGTATCTGGGAATAGTGCTGAGACAGGACTGAAA GAGACAAATAGCTTGAAGCTGGAAGGTATAAAGAAATTGTGGGGGAAAGAAGGCTATCTTCCCAAGAAG GAAAGCAAAACTGGTGATGAAAGTGGAGCTCTGCCTGTTCCTCAAGAGAGTATAATGGAGAATGTAGAT CAAGCTATAACTAAAAAGGATCAATCTCAAGTTCTTACCCAATCTAAAGAGGAGAAAGAAAAGCAGCTG CTGGCATCATCATTATTTGTTGGTCTAGGATCAGAAAGTACAATCAACCTGCTGGGAAAAGCAGATACT GTCTCTCACAAGTTCAGAAGGAAATCAAAAGTCAAAGAAGCTAAAAGTGGCGAAACAACCAGTACTCAT AATATGACCTGTTCTTCCTTTAGTTCTTTGTCAAATGTGGCATATGAAGATGATTATTATTCGAATACT TTGCACGATACAGGAGACAAGGAATTAAAGAAATTTTCTCTCACTTCAGAACTTTTGGATTCTGAGTCA CTCACAGAACTGCCCTTGGTTGAGAAATTCTCATATTGTAGTCTGTCTACACCTTCATTGTTTGCTAAT AACAACATGGAAATTTTTCACCCTCCTCAATCTACTGCAGCCTCAGTTGCCAAGGAAAGCTCTTTAGCT TCATCTTTTTTGGAAGAAACTACTGAATACATACACTCAAATGCTATGGAAGTCTGTAATAATGAAACT ATATCAGTGTCTTCTTATAAAATTTGGAAAGATGATTGTTTATTGATGGTCTGGTCAGTCACTAATAAG AGTGGTTTGGAATTGAAAAGTGCTGACTTAGAAATTTTTCCTGCAGAAAATTTCAAGGTGACTGAGCAA CCTGGATGCTGTTTGCCTGTAATGGAAGCAGAAAGCACCAAAAGCTTTCAATATAGTGTGCAGATAGAA AAACCTTTTACAGAAGGAAATCTTACTGGTTTTATTAGTTATCATATGATGGATACTCATTCTGCTCAG CTGGAATTTTCTGTAAACTTATCACTATTAGATTTCATTAGACCATTAAAAATCTCAAGTGACGACTTT GGGAAACTCTGGTTATCCTTCGCAAATGATGTGAAACAAAATGTAAAAATGTCAGAATCTCAAGCTGCA CTTCCTTCTGCACTAAAGACTCTGCAACAGAAACTAAGACTCCATATTATTGAGATTATAGGCAATGAA GGGCTATTGGCCTGTCAGCTGCTCCCATCCATCCCCTGCTTACTGCATTGCCGAGTTCATGCAGATGTA TTAGCCCTGTGGTTCAGATCCTCCTGTTCTACTCTTCCTGACTATTTACTGTATCAGTGTCAAAAGGTG ATGGAGGGATCCTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001252127
Insert Size	3189 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001252127.1
RefSeq Size	6791 bp
RefSeq ORF	3189 bp
Locus ID	23431
UniProt ID	Q9UPM8
Protein Pathways	Lysosome
MW	119.5 kDa
Gene Summary	This gene encodes a member of the adaptor complexes large subunit protein family. These proteins are components of the heterotetrameric adaptor protein complexes, which play important roles in the secretory and endocytic pathways by mediating vesicle formation and sorting of integral membrane proteins. The encoded protein is a large subunit of adaptor protein complex-4, which is associated with both clathrin- and nonclathrin-coated vesicles. Disruption of this gene may be associated with cerebral palsy. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011] Transcript Variant: This variant (2) uses an alternate splice site and a downstream, in-frame start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1.

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