Kinesin Heavy Chain 2 (KIF2A) (NM_001243953) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CDCBM3; HK2; KIF2
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC330199 representing NM_001243953. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGCAACGGCCAACTTCGGCAAGATCCAGATCGGGATTTACGTGGAGATCAAGCGCAGCGATGGCCGA ATACATCAAGCAATGGTAACATCTTTAAATGAAGATAATGAAAGTGTAACTGTTGAATGGATAGAAAAT GGAGATACAAAAGGCAAAGAGATTGACCTGGAGAGCATCTTTTCACTTAACCCTGACCTTGTTCCTGAT GAAGAAATTGAACCCAGTCCAGAAACACCTCCACCTCCAGCATCCTCAGCCAAAGTAAACAAAATTGTA AAGAATCGACGGACTGTAGCTTCTATTAAGAATGACCCTCCTTCAAGAGATAATAGAGTGGTTGGTTCA GCACGTGCACGGCCCAGTCAATTTCCTGAACAGTCTTCCTCTGCACAACAGAATGCACGTAGAAAATCT AATTGTGTGAAAGAAGTAGAAAAACTGCAAGAAAAACGAGAGAAAAGGAGATTGCAACAGCAAGAACTT AGAGAAAAAAGAGCCCAGGACGTTGATGCTACAAACCCAAATTATGAAATTATGTGTATGATCAGAGAC TTTAGAGGAAGTTTGGATTATAGACCATTAACAACAGCAGATCCTATTGATGAACATAGGATATGTGTG TGTGTAAGAAAACGACCACTCAATAAAAAAGAAACTCAAATGAAAGATCTTGATGTAATCACAATTCCT AGTAAAGATGTTGTGATGGTACATGAACCAAAACAAAAAGTAGATTTAACAAGGTACCTAGAAAACCAA ACATTTCGTTTTGATTATGCCTTTGATGACTCAGCTCCTAATGAAATGGTTTACAGGTTTACTGCTAGA CCACTAGTGGAAACTATATTTGAAAGGGGAATGGCTACATGCTTTGCTTATGGGCAGACTGGAAGTGGA AAAACTCATACTATGGGTGGTGACTTTTCAGGAAAGAACCAAGATTGTTCTAAAGGAATTTATGCATTA GCAGCTCGAGATGTCTTTTTAATGCTAAAGAAGCCAAACTATAAGAAGCTAGAACTTCAAGTATATGCA ACCTTCTTTGAAATTTATAGTGGAAAGGTGTTTGACTTGCTAAACAGGAAAACAAAATTAAGAGTTCTA GAAGATGGAAAACAGCAGGTTCAAGTGGTGGGATTACAGGAACGGGAGGTCAAATGTGTTGAAGATGTA CTGAAACTCATTGACATAGGCAACAGTTGCAGAACATCCGGTCAAACATCTGCAAATGCACATTCATCT CGGAGCCATGCAGTGTTTCAGATTATTCTTAGAAGGAAAGGAAAACTACATGGCAAATTTTCTCTCATT GATTTGGCTGGAAATGAAAGAGGAGCTGATACTTCCAGTGCGGACAGGCAAACTAGGCTTGAAGGTGCT GAAATTAATAAAAGCCTTTTAGCACTCAAGGAGTGCATCAGAGCCTTAGGTAGAAATAAACCTCATACT CCTTTCCGTGCAAGTAAACTCACTCAGGTGTTAAGAGATTCTTTCATAGGTGAAAACTCTCGTACCTGC ATGATTGCCACAATCTCTCCAGGAATGGCATCCTGTGAAAATACTCTTAATACATTAAGATATGCAAAT AGGGTCAAAGAATTGACTGTAGATCCAACTGCTGCTGGTGATGTTCGTCCAATAATGCACCATCCACCA AACCAGATTGATGACTTAGAGACACAGTGGGGTGTGGGGAGTTCCCCTCAGAGAGATGATCTAAAACTT CTTTGTGAACAAAATGAAGAAGAAGTCTCTCCACAGTTGTTTACTTTCCACGAAGCTGTTTCACAAATG GTAGAAATGGAAGAACAAGTTGTAGAAGATCACAGGGCAGTGTTCCAGGAATCTATTCGGTGGTTAGAA GATGAAAAGGCCCTCTTAGAGATGACTGAAGAAGTAGATTATGATGTCGATTCATATGCTACACAACTT GAAGCTATTCTTGAGCAAAAAATAGACATTTTAACTGAACTGCGGGATAAAGTGAAATCTTTCCGTGCA GCTCTACAAGAGGAGGAACAAGCCAGCAAGCAAATCAACCCGAAGAGACCCCGTGCCCTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001243953
Insert Size	2064 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001243953.1
RefSeq Size	4001 bp
RefSeq ORF	2064 bp
Locus ID	3796
Protein Families	Druggable Genome
MW	78 kDa
Gene Summary	The protein encoded by this gene is a plus end-directed motor required for normal mitotic progression. The encoded protein is required for normal spindle activity during mitosis and is necessary for normal brain development. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011] Transcript Variant: This variant (4) uses an alternate in-frame splice junction at the 3' end of an exon and lacks an alternate in-frame exon compared to isoform 2. The resulting isoform (4) has the same N- and C-termini but lacks two alternate internal segments compared to isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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