BTN3A3 (NM_001242803) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	BTF3; BTN3.3
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC330018 representing NM_001242803. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGTGGGTGAAGACGCTGATCTGCCCTGTCACCTGTTCCCGACCATGAGTGCAGAGACCATGGAGCTG AGGTGGGTGAGTTCCAGCCTAAGGCAGGTGGTGAACGTGTATGCAGATGGAAAGGAAGTGGAAGACAGG CAGAGTGCACCGTATCGAGGGAGAACTTCGATTCTGCGGGATGGCATCACTGCAGGGAAGGCTGCTCTC CGAATACACAACGTCACAGCCTCTGACAGTGGAAAGTACTTGTGTTATTTCCAAGATGGTGACTTCTAC GAAAAAGCCCTGGTGGAGCTGAAGGTTGCAGAGTGGAGGAAAATCCAGTACATGGCTCGTGGAGAGAAG TCTTTGGCCTATCATGAATGGAAAATGGCCCTCTTCAAACCTGCGGATGTGATTCTGGATCCAGACACG GCAAACGCCATCCTCCTTGTTTCTGAGGACCAGAGGAGTGTGCAGCGTGCTGAAGAGCCGCGGGATCTG CCAGACAACCCTGAGAGATTTGAATGGCGTTACTGTGTCCTTGGCTGTGAAAACTTCACATCAGGGAGA CATTACTGGGAGGTGGAAGTGGGGGACAGAAAAGAGTGGCATATTGGGGTATGTAGTAAGAACGTGGAG AGGAAAAAAGGTTGGGTCAAAATGACACCGGAGAACGGATACTGGACTATGGGCCTGACTGATGGGAAT AAGTATCGGGCTCTCACTGAGCCCAGAACCAACCTGAAACTTCCTGAGCCTCCTAGGAAAGTGGGGATC TTCCTGGACTATGAGACTGGAGAGATCTCGTTCTATAATGCCACAGATGGATCTCATATCTACACCTTT CCGCACGCCTCTTTCTCTGAGCCTCTATATCCTGTTTTCAGAATTTTGACCTTGGAGCCCACTGCCCTG ACCATTTGCCCAATACCAAAAGAAGTAGAGAGTTCCCCCGATCCTGACCTAGTGCCTGATCATTCCCTG GAGACACCACTGACCCCGGGCTTAGCTAATGAAAGTGGGGAGCCTCAGGCTGAAGTAACATCTCTGCTT CTCCCTGCCCACCCTGGAGCTGAGGTCTCCCCTTCTGCAACAACCAATCAGAACCATAAGCTACAGGCA CGCACTGAAGCACTTTACTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001242803
Insert Size	1125 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001242803.1
RefSeq Size	2364 bp
RefSeq ORF	1125 bp
Locus ID	10384
UniProt ID	O00478
Protein Families	Druggable Genome, Transmembrane
MW	42.3 kDa
Gene Summary	The butyrophilin (BTN) genes are a group of major histocompatibility complex (MHC)-associated genes that encode type I membrane proteins with 2 extracellular immunoglobulin (Ig) domains and an intracellular B30.2 (PRYSPRY) domain. Three subfamilies of human BTN genes are located in the MHC class I region: the single-copy BTN1A1 gene (MIM 601610) and the BTN2 (e.g., BTN2A1; MIM 613590) and BTN3 (e.g., BNT3A3) genes, which have undergone tandem duplication, resulting in 3 copies of each (summary by Smith et al., 2010 [PubMed 20208008]).[supplied by OMIM, Nov 2010] Transcript Variant: This variant (3) lacks several exons in two regions, but the open reading frame is retained, compared to variant 1. The encoded isoform (c) has a shorter N-terminus and lacks an internal segment, compared to isoform a.

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