ODF2 (NM_001242354) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CT134; ODF2/1; ODF2/2; ODF84
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC329934 representing NM_001242354. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGAAGGACCGCTCTTCAACTCCCCCCTTACATGTTCACGTGGATGAGAACACCCCTGTCCACGTCCAC ATAAAAAAACTCCCGAAACCATCAGCGACCAGCAGCCAGAAATCTCACAAGCGAGGAATGAAAGGGGAC ACTGTGAATGTGCGGCGGAGTGTCCGGGTGAAAACCAAGGTCAAGATGCAAAAAGGTGAGCGCCAGATG GCCAAAAGGTTCCTGGAGGAACGGAAGGAAGAGCTGGAGGAGGTGGCCCACGAACTGGCTGAGACTGAG CACGAGAACACGGTGTTGAGGCACAACATCGAGCGCATGAAGGAGGAGAAGGACTTCACCATACTTCAG AAGAAACACCTACAACAGGAGAAGGAGTGCCTCATGTCCAAGCTGGTGGAGGCGGAAATGGATGGGGCT GCGGCTGCCAAGCAGGTCATGGCCTTGAAGGATACCATCGGGAAGCTGAAAACGGAGAAACAAATGACC TGCACGGACATCAACACCCTGACAAGGCAGAAGGAACTTCTCCTGCAGAAGCTGAGCACATTTGAGGAG ACCAACCGCACCCTCCGAGACCTCCTGAGGGAACAGCACTGCAAAGAGGATTCTGAAAGACTAATGGAG CAACAAGGAGCACTGCTGAAACGGCTGGCGGAGGCCGACTCAGAGAAAGCGCGCCTGCTGTTACTGCTG CAAGACAAGGACAAGGAGGTGGAAGAGCTCCTTCAGGAAATACAATGTGAGAAGGCTCAAGCAAAGACA GCCTCTGAGCTTTCTAAATCCATGGAGTCCATGCGTGGGCATTTGCAGGCACAGCTTCGGTCCAAAGAG GCTGAGAACAGTCGCCTGTGCATGCAGATTAAGAATCTGGAGCGCAGCGGGAATCAGCATAAGGCAGAA GTGGAGGCCATCATGGAGCAGCTGAAGGAGTTGAAGCAGAAGGGAGACCGAGACAAAGAGAGCTTGAAG AAGGCCATCCGAGCCCAGAAGGAGCGAGCCGAGAAGAGCGAGGAGTATGCTGAGCAGCTACACGTGCAA CTCGCTGACAAGGATCTTTATGTCGCTGAAGCTTTATCCACTCTGGAATCCTGGAGGAGCCGCTACAAC CAAGTTGTAAAAGAAAAGGGAGACCTTGAGCTGGAAATTATTGTCCTGAATGACCGGGTAACAGATCTT GTAAACCAACAACAAACCCTGGAGGAGAAGATGCGGGAAGACCGGGATAGCCTGGTGGAGAGACTACAC CGTCAGACTGCTGAGTATTCCGCATTCAAGCTGGAGAATGAGAGGCTGAAGGCCAGCTTTGCTCCAATG GAGGACAAACTCAACCAGGCACACCTCGAGGTCCAGCAGCTGAAGGCCTCAGTGAAGAACTATGAGGGG ATGATTGACAACTATAAGAGTCAGGTGATGAAGACCAGATTGGAGGCTGATGAAGTAGCTGCCCAGCTA GAACGCTGTGACAAAGAGAACAAGATCCTTAAAGATGAGATGAACAAAGAGATTGAGGCGGCACGAAGG CAGTTCCAGTCTCAGCTGGCTGACCTGCAGCAGCTCCCTGACATCCTGAAGATCACGGAGGCGAAGCTG GCTGAGTGCCAAGACCAACTGCAGGGCTATGAGCGGAAGAACATCGACCTCACAGCCATCATATCAGAC CTGCGCAGCCGGGTAAGGGACTGGCAGAAAGGGTCCCACGAACTGACCCGAGCAGGGGCCCGCATACCA AGATGA
Restriction Sites	SgfI-MluI
ACCN	NM_001242354
Insert Size	1731 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001242354.1
RefSeq Size	2227 bp
RefSeq ORF	1731 bp
Locus ID	4957
UniProt ID	Q5BJF6
MW	67 kDa
Gene Summary	The outer dense fibers are cytoskeletal structures that surround the axoneme in the middle piece and principal piece of the sperm tail. The fibers function in maintaining the elastic structure and recoil of the sperm tail as well as in protecting the tail from shear forces during epididymal transport and ejaculation. Defects in the outer dense fibers lead to abnormal sperm morphology and infertility. This gene encodes one of the major outer dense fiber proteins. Alternative splicing results in multiple transcript variants. The longer transcripts, also known as 'Cenexins', encode proteins with a C-terminal extension that are differentially targeted to somatic centrioles and thought to be crucial for the formation of microtubule organizing centers. [provided by RefSeq, Oct 2010] Transcript Variant: This variant (8) uses an alternate splice site in the 5' coding region, lacks an alternate in-frame exon, and differs in the 3' UTR and coding sequence, compared to variant 1. The resulting isoform (8) is shorter at the N-terminus, lacks an alternate internal segment, and has a shorter and distinct C-terminus compared to isoform 1.

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