DGKH (NM_001204506) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DGKeta
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC329760 representing NM_001204506. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGCTGTGTGCTGAGAACAGAAAGGAGATGGAGGATTGGATCAGCTCACTGAAGTCTGTACAGACCAGA GAACCCTACGAGGTGGCCCAGTTTAATGTGGAACATTTCTCAGGGATGCACAACTGGTACGCCTGCTCC CACGCCCGACCCACCTTCTGTAACGTGTGCAGAGAGAGTCTTTCTGGAGTCACCTCCCATGGCCTGTCC TGCGAAGTGTGTAAATTCAAGGCTCACAAAAGATGTGCAGTGAGAGCAACAAATAACTGTAAATGGACT ACCCTGGCCTCCATCGGGAAGGACATTATAGAAGATGAAGATGGCGTCGCGATGCCTCACCAGTGGCTT GAGGGCAACCTGCCTGTAAGTGCCAAGTGTGCTGTCTGCGACAAAACATGTGGCAGTGTTCTCCGTCTA CAGGATTGGAAATGCCTTTGGTGTAAGACAATGGTACACACTGCCTGCAAAGATTTATACCATCCAATA TGTCCACTTGGTCAATGTAAAGTATCTATCATACCTCCAATTGCACTAAACAGCACCGATTCCGATGGT TTCTGTAGAGCAACATTTTCGTTCTGTGTTAGTCCTCTATTGGTTTTTGTCAATTCTAAGAGTGGAGAT AATCAGGGAGTAAAGTTCCTCCGTCGCTTTAAACAGTTGCTAAATCCGGCTCAGGTGTTTGATTTAATG AATGGAGGTCCTCATTTAGGTTTAAGATTATTTCAGAAGTTTGACAATTTCCGGATTCTTGTTTGTGGA GGCGATGGAAGTGTAGGTTGGGTTTTGTCAGAAATCGATAAGCTCAACTTGAATAAACAGTGTCAGCTG GGAGTGTTGCCTTTGGGTACAGGAAATGACCTTGCCCGAGTTCTTGGCTGGGGAGGTTCATATGACGAT GACACCCAACTTCCTCAGATCCTAGAGAAACTGGAACGAGCCAGTACCAAAATGTTGGACAGGTGGAGT ATAATGACATATGAACTCAAATTGCCACCAAAAGCTTCCCTACTTCCAGGACCTCCAGAAGCATCTGAA GAATTTTATATGACGATTTATGAAGACTCAGTTGCAACGCATCTTACAAAAATCCTCAATTCTGATGAA CATGCAGTGGTCATATCTTCTGCCAAGACGCTATGTGAAACTGTAAAGGACTTCGTTGCCAAAGTAGAA AAGACGTATGACAAAACCTTGGAAAATGCCGTTGTAGCTGATGCCGTGGCCAGTAAATGTTCAGTCCTA AACGAGAAGCTCGAACAACTGCTGCAGGCTTTGCACACAGATTCCCAGGCTGCGCCTGTTCTCCCTGGC CTCAGCCCTCTCATTGTGGAAGAAGATGCTGTGGAATCGTCCAGTGAAGAGTCCCTGGGTGAAAGCAAA GAGCAGCTTGGGGATGACGTTACAAAACCTTCCTCCCAGAAAGCCGTCAAACCAAGGGAAATCATGTTG CGGGCAAATAGTTTAAAGAAAGCAGTGAGGCAAGTCATTGAGGAAGCCGGAAAAGTTATGGATGACCCG ACAGTTCACCCCTGTGAACCAGCTAATCAGTCCTCTGATTATGACAGCACAGAAACAGATGAATCTAAG GAGGAAGCTAAAGATGATGGTGCCAAAGAATCAATAACTGTTAAAACTGCACCTCGGTCTCCAGATGCC CGGGCAAGTTATGGCCATTCCCAAACTGATTCTGTCCCTGGTCCAGCTGTGGCAGCCAGCAAAGAAAAC CTCCCTGTGCTCAATACCAGAATAATCTGCCCAGGTTTAAGAGCAGGACTGGCTGCCTCAATTGCTGGG AGTTCGATTATCAACAAAATGTTACTGGCAAACATTGATCCTTTTGGTGCCACGCCGTTTATTGACCCG GATCTAGATTCCGTAGATGGATATTCAGAAAAATGTGTCATGAACAATTACTTTGGGATTGGATTAGAT GCAAAAATTTCATTAGAATTTAATAATAAAAGAGAGGAGCACCCTGAAAAATGCAGGAGCCGAACTAAA AACTTGATGTGGTATGGAGTCCTTGGAACCCGGGAGTTATTACAGAGATCGTACAAGAATTTAGAACAA AGGGTTCAACTTGAGTGTGATGGGCAGTATATTCCTCTTCCCAGCTTGCAAGGCATAGCCGTGTTGAAC ATTCCCAGCTATGCTGGAGGCACTAACTTTTGGGGTGGAACTAAAGAGGATGATATATTTGCTGCACCA TCCTTTGATGACAAGATCCTGGAAGTTGTAGCAATATTTGATAGCATGCAAATGGCAGTTTCAAGGGTC ATTAAACTGCAGCATCATCGAATAGCCCAGTGCCGTACAGTGAAAATCACTATATTTGGTGACGAAGGA GTCCCAGTGCAAGTGGATGGTGAAGCGTGGGTTCAGCCTCCAGGGATTATCAAAATTGTGCACAAAAAC AGAGCACAAATGCTAACAAGGGACAGAGCCTTTGAGAGCACTCTGAAATCTTGGGAAGATAAGCAGAAG TGTGATTCTGGTAAACCAGTTCTCCGAACCCATTTGTACATCCATCACGCCATTGACTTGGCAACAGAA GAGGTGTCGCAGATGCAGCTATGCTCCCAGGCTGCAGAGGAGCTCATTACTAGGATATGTGACGCAGCC ACAATTCACTGTCTTTTGGAGCAAGAACTGGCCCATGCTGTGAATGCCTGCTCCCATGCCCTGAATAAA GCCAACCCAAGGTGCCCGGAGAGTCTTACAAGAGACACTGCCACTGAAATAGCCATCAATGTGAAGGCG CTGTATAATGAAACAGAATCTTTGCTAGTTGGCAGGGTTCCTTTGCAGCTGGAATCGCCACATGAAGAG CGAGTATCCAATGCCTTACACTCTGTGGAGGTGGAATTACAGAAACTGACAGAGATTCCTTGGCTTTAT TATATCTTACACCCAAATGAGGATGAGGAACCTCCTATGGATTGCACCAAAAGGAACAACAGAAGCACC GTATTTCGAATAGTGCCAAAGTTTAAAAAGGAAAAGGTTCAGAAGCAGAAGACAAGTTCACAGCCTGTT CAGAAATGGGGCACAGAGGAAGTTGCTGCTTGGCTGGATCTGCTCAATTTGGGAGAGTACAAAGATATC TTCATCCGTCATGACATCAGAGGGGCTGAACTTTTGCATCTGGAAAGGCGAGATCTTAAGGATCTGGGG ATACCGAAAGTGGGTCATGTGAAGCGAATTCTCCAGGGAATTAAAGAGCTTGGAAGGAGCACTCCACAG TCGGAGGTGTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001204506
Insert Size	3255 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001204506.1
RefSeq Size	3956 bp
RefSeq ORF	3255 bp
Locus ID	160851
UniProt ID	Q86XP1
Protein Families	Druggable Genome
Protein Pathways	Glycerolipid metabolism, Glycerophospholipid metabolism, Metabolic pathways, Phosphatidylinositol signaling system
MW	120.6 kDa
Gene Summary	This gene encodes a member of the diacylglycerol kinase (DGK) enzyme family. Members of this family are involved in regulating intracellular concentrations of diacylglycerol and phosphatidic acid. Variation in this gene has been associated with bipolar disorder. Alternatively spliced transcript variants have been identified. [provided by RefSeq, Jul 2014] Transcript Variant: This variant (5) differs in the 5' UTR, lacks a portion of the 5' coding region, and contains an alternate exon in the 3' coding region, which results in a frameshift, compared to variant 1. It initiates translation at a downstream in-frame start codon. The encoded isoform (4) has a shorter N-terminus and a distinct C-terminus, and is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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