SAP97 (DLG1) (NM_001204386) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DLGH1; hdlg; SAP-97; SAP97
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC329744 representing NM_001204386. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGCCGGTCCGGAAGCAAGATACCCAGAGAGCATTGCACCTTTTGGAGGAATATCGTTCAAAACTAAGC CAAACTGAAGACAGACAGCTCAGAAGTTCCATAGAACGGGTTATTAACATATTTCAGAGCAACCTCTTT CAGGCTTTAATAGATATTCAAGAATTTTATGAAGTGACCTTACTGGATAATCCAAAATGTATAGATCGT TCAAAGCCGTCTGAACCAATTCAACCTGTGAATACTTGGGAGATTTCCAGCCTTCCAAGCTCTACTGTG ACTTCAGAGACACTGCCAAGCAGCCTTAGCCCTAGTGTAGAGAAATACAGGTATCAGGATGAAGATACA CCTCCTCAAGAGCATATTTCCCCACAAATCACAAATGAAGTGATAGGTCCAGAATTGGTTCATGTCTCA GAGAAGAACTTATCAGAGATTGAGAATGTCCATGGATTTGTTTCTCATTCTCATATTTCACCAATAAAG GCAAATCCTCCCCCAGTACTGGTCAACACAGATAGCTTGGAAACACCAACTTACGTTAATGGCACAGAT GCAGATTATGAATATGAAGAAATCACACTTGAAAGGGGAAATTCAGGGCTTGGTTTCAGCATTGCAGGA GGTACGGACAACCCACACATTGGAGATGACTCAAGTATTTTCATTACCAAAATTATCACAGGGGGAGCA GCCGCCCAAGATGGAAGATTGCGGGTCAATGACTGTATATTACGAGTAAATGAAGTAGATGTTCGTGAT GTAACACATAGCAAAGCAGTTGAAGCGTTGAAAGAAGCAGGGTCTATTGTACGCTTGTATGTAAAAAGA AGGAAACCAGTGTCAGAAAAAATAATGGAAATAAAGCTCATTAAAGGTCCTAAAGGTCTTGGGTTTAGC ATTGCTGGAGGTGTTGGAAATCAGCATATTCCTGGGGATAATAGCATCTATGTAACCAAAATAATTGAA GGAGGTGCAGCACATAAGGATGGCAAACTTCAGATTGGAGATAAACTTTTAGCAGTGAATAACGTATGT TTAGAAGAAGTTACTCATGAAGAAGCAGTAACTGCCTTAAAGAACACATCTGATTTTGTTTATTTGAAA GTGGCAAAACCCACAAGTATGTATATGAATGATGGCTATGCACCACCTGATATCACCAACTCTTCTTCT CAGCCTGTTGATAACCATGTTAGCCCATCTTCCTTCTTGGGCCAGACACCAGCATCTCCAGCCAGATAC TCCCCAGTTTCTAAAGCAGTACTTGGAGATGATGAAATTACAAGGGAACCTAGAAAAGTTGTTCTTCAT CGTGGCTCAACGGGCCTTGGTTTCAACATTGTAGGAGGAGAAGATGGAGAAGGAATATTTATTTCCTTT ATCTTAGCCGGAGGACCTGCTGATCTAAGTGGAGAGCTCAGAAAAGGAGATCGTATTATATCGGTAAAC AGTGTTGACCTCAGAGCTGCTAGTCATGAGCAGGCAGCAGCTGCATTGAAAAATGCTGGCCAGGCTGTC ACAATTGTTGCACAATATCGACCTGAAGAATACAGTCGTTTTGAAGCTAAAATACATGATTTACGGGAG CAGATGATGAATAGTAGTATTAGTTCAGGGTCAGGTTCTCTTCGAACTAGCCAGAAGCGATCCCTCTAT GTCAGAGCCCTTTTTGATTATGACAAGACTAAAGACAGTGGGCTTCCCAGTCAGGGACTGAACTTCAAA TTTGGAGATATCCTCCATGTTATTAATGCTTCTGATGATGAATGGTGGCAAGCCAGGCAGGTTACACCA GATGGTGAGAGCGATGAGGTCGGAGTGATTCCCAGTAAACGCAGAGTTGAGAAGAAAGAACGAGCCCGA TTAAAAACAGTGAAATTCAATTCTAAAACGAGAGATAAAGGGTCATTCAATGACAAGCGTAAAAAGAAC CTCTTTTCCCGAAAATTCCCCTTCTACAAGAACAAGGACCAGAGTGAGCAGGAAACAAGTGATGCTGAC CAGCATGTAACTTCTAATGCCAGCGATAGTGAAAGTAGTTACCGTGGTCAAGAAGAATACGTCTTATCT TATGAACCAGTGAATCAACAAGAAGTTAATTATACTCGACCAGTGATCATATTGGGACCTATGAAAGAC AGGATAAATGATGACTTGATCTCAGAATTTCCTGACAAATTTGGATCCTGTGTTCCTCATACAACTAGA CCAAAACGAGATTATGAGGTAGATGGAAGAGATTATCATTTTGTGACTTCAAGAGAGCAGATGGAAAAA GATATCCAGGAACATAAATTCATTGAAGCTGGCCAGTATAACAATCATCTATATGGAACAAGTGTTCAG TCTGTACGAGAAGTAGCAGAAAAGGGCAAACACTGTATCCTTGATGTGTCTGGAAATGCCATAAAGAGA TTACAGATTGCACAGCTTTACCCTATCTCCATTTTTATTAAACCCAAATCCATGGAAAATATCATGGAA ATGAATAAGCGTCTAACAGAAGAACAAGCCAGAAAAACATTTGAGAGAGCCATGAAACTGGAACAGGAG TTTACTGAACATTTCACAGCTATTGTACAGGGGGATACGCTGGAAGACATTTACAACCAAGTGAAACAG ATCATAGAAGAACAATCTGGTTCTTACATCTGGGTTCCGGCAAAAGAAAAGCTATGA
Restriction Sites	SgfI-MluI
ACCN	NM_001204386
Insert Size	2679 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001204386.1
RefSeq Size	4941 bp
RefSeq ORF	2679 bp
Locus ID	1739
UniProt ID	Q12959
Protein Families	Druggable Genome
Protein Pathways	T cell receptor signaling pathway
MW	99.8 kDa
Gene Summary	This gene encodes a multi-domain scaffolding protein that is required for normal development. This protein may have a role in septate junction formation, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. A multitude of transcript variants deriving from alternative splicing and the use of multiple alternate promoter have been observed, including some splice variants that may be specific to brain and other tissues. An upstream uORF may regulate translation at some splice variants of this gene. [provided by RefSeq, Sep 2018] Transcript Variant: This variant (3) is missing an in-frame coding exon, and contains an alternate in-frame coding exon compared to variant 1. This results in a shorter isoform (3) compared to isoform 1.

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