PREI3 (MOB4) (NM_001204094) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	2C4D; CGI-95; MOB1; MOB3; MOBKL3; PHOCN; PREI3
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC329704 representing NM_001204094. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGACAGTACACTAGCTGTTCAACAGTATATTCAACAGAACATAAGAGCAGATTGCTCCAATATTGAC AAAATTCTTGAACCACCTGAAGGCCAAGATGAAGGTGTGTGGAAGTATGAACATTTAAGGCAGTTCTGC CTTGAGCTAAATGGACTTGCTGTCAAACTTCAGAGTGAATGCCATCCAGATACTTGCACTCAAATGACA GCAACTGAACAATGGATTTTTCTTTGTGCAGCTCATAAAACTCCAAAAGAGTGTCCTGCTATAGACTAT ACTAGACACACACTTGATGGTGCTGCATGTCTTCTGAATAGCAATAAATATTTTCCCAGCAGGGTTAGC ATAAAGGAATCATCTGTAGCGAAACTAGGATCAGTATGCCGTAGGATTTACAGAATATTTTCACATGCT TATTTTCATCATCGGCAGATATTTGATGAATATGAAAATGAAACATTTTTGTGTCATCGGTTTACTAAG TTTGTGATGAAATACAATTTGATGTCCAAGGATAACCTGATTGTACCAATTTTAGAAGAGGAAGTACAG AATTCAGTTTCTGGGGAAAGTGAAGCATGA
Restriction Sites	SgfI-MluI
ACCN	NM_001204094
Insert Size	582 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001204094.1
RefSeq Size	3742 bp
RefSeq ORF	582 bp
Locus ID	25843
UniProt ID	Q9Y3A3
MW	22.3 kDa
Gene Summary	This gene was identified based on its similarity with the mouse counterpart. Studies of the mouse counterpart suggest that the expression of this gene may be regulated during oocyte maturation and preimplantation following zygotic gene activation. Alternatively spliced transcript variants encoding distinct isoforms have been observed. Naturally occurring read-through transcription occurs between this locus and the neighboring locus HSPE1.[provided by RefSeq, Feb 2011] Transcript Variant: This variant (4) uses an alternate exon at its 5' end compared to variant 1, resulting in a different 5' UTR and translation initiation at a downstream start codon. The resulting isoform (2) has a shorter N-terminus compared to isoform 1. Variants 2 and 4 encode the same isoform (2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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