ARSF (NM_001201538) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ASF
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC329632 representing NM_001201538. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGAGGCCCAGGAGACCCTTGGTCTTCATGTCTTTGGTGTGTGCACTCTTGAACACATGCCAGGCACAC AGGGTGCATGACGACAAGCCTAATATTGTCCTAATCATGGTTGATGACCTGGGTATTGGAGATCTGGGC TGCTACGGCAATGACACCATGAGGACGCCTCACATCGACCGCCTTGCCAGGGAAGGCGTGCGACTGACT CAGCACATCTCTGCCGCCTCCCTCTGCAGCCCAAGCCGGTCCGCGTTCTTGACGGGAAGATACCCCATC CGATCAGGTATGGTTTCTAGTGGTAATAGACGTGTCATCCAAAATCTTGCAGTCCCCGCAGGCCTCCCT CTTAATGAGACAACACTTGCAGCCTTGCTAAAGAAGCAAGGATACAGCACGGGGCTTATAGGCAAATGG CACCAAGGCTTGAACTGCGACTCCCGAAGTGACCAGTGCCACCATCCATATAATTATGGGTTTGACTAC TACTATGGCATGCCGTTCACTCTCGTTGACAGCTGCTGGCCGGACCCCTCTCGTAACACGGAATTAGCC TTTGAGAGTCAGCTCTGGCTCTGTGTGCAGCTAGTTGCCATTGCCATCCTCACCCTAACCTTTGGGAAG CTGAGCGGCTGGGTCTCTGTTCCCTGGCTCCTGATCTTCTCCATGATTCTGTTTATTTTCCTCTTGGGC TATGCTTGGTTCTCCAGCCACACGTCCCCTTTATACTGGGACTGCCTCCTCATGCGGGGGCACGAGATC ACGGAGCAGCCCATGAAGGCTGAACGAGCTGGATCCATTATGGTGAAGGAAGCGATTTCCTTTTTAGAA AGGCACAGTAAGGAAACTTTCCTTCTCTTTTTCTCCTTTCTTCACGTGCACACACCTCTCCCCACCACG GACGATTTCACTGGCACCAGCAAGCATGGCTTGTATGGGGATAATGTGGAAGAGATGGACTCCATGGTG GGCAAGATTCTTGATGCTATCGATGATTTTGGCCTAAGGAACAACACCCTTGTCTACTTTACATCAGAT CACGGAGGGCATTTGGAAGCTAGGCGAGGGCATGCCCAACTTGGTGGATGGAATGGAATATACAAAGGT GGAAAAGGCATGGGGGGCTGGGAAGGTGGAATCCGCGTCCCAGGAATTGTCCGATGGCCTGGAAAGGTA CCAGCTGGACGGTTGATTAAGGAACCTACAAGTTTAATGGATATTTTACCAACTGTCGCATCAGTGTCA GGAGGAAGTCTCCCTCAGGACAGGGTCATTGACGGCCGAGACCTCATGCCCTTGCTGCAGGGCAACGTC AGGCACTCGGAGCATGAATTTCTTTTCCACTACTGTGGCTCCTACCTGCACGCCGTGCGGTGGATCCCC AAGGACGACAGTGGGTCAGTTTGGAAGGCTCACTATGTGACCCCGGTATTCCAGCCACCAGCTTCTGGT GGCTGCTATGTCACCTCATTATGCAGATGTTTCGGAGAACAGGTTACCTACCACAACCCCCCTCTGCTC TTCGATCTCTCCAGGGACCCCTCAGAGTCCACACCCCTGACACCTGCCACAGAGCCCCTCCATGATTTT GTGATTAAAAAGGTGGCCAACGCCCTGAAGGAACACCAGGAAACCATCGTGCCTGTGACCTACCAACTC TCAGAACTGAATCAGGGCAGGACGTGGCTGAAGCCTTGCTGTGGGGTGTTCCCATTTTGTCTGTGTGAC AAGGAAGAGGAAGTCTCTCAGCCTCGGGGTCCTAACGAGAAGAGATAA
Restriction Sites	SgfI-MluI
ACCN	NM_001201538
Insert Size	1773 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001201538.1
RefSeq Size	2082 bp
RefSeq ORF	1773 bp
Locus ID	416
UniProt ID	P54793
Protein Families	Druggable Genome, Secreted Protein, Transmembrane
MW	65.9 kDa
Gene Summary	This gene is a member of the sulfatase family, and more specifically, the arylsulfatase subfamily. Members of the subfamily share similarity in sequence and splice sites, and are clustered together on chromosome X, suggesting that they are derived from recent gene duplication events. Sulfatases are essential for the correct composition of bone and cartilage matrix. The activity of this protein, unlike that of arylsulfatase E, is not inhibited by warfarin. Multiple alternatively spliced variants, encoding the same protein, have been identified.[provided by RefSeq, Jan 2011] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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