DAP3 (NM_001199851) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	bMRP-10; DAP-3; MRP-S29; MRPS29; S29mt
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC329575 representing NM_001199851. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGATGCTGAAAGGAATAACAAGGCTTATCTCTAGGATCCATAAGGCCAAGCATGGGGATCAGCACGAG GGTCAGCACTACAACATCTCCCCCCAGGATTTGGAGACTGTATTTCCCCATGGCCTTCCTCCTCGCTTT GTGATGCAGGTGAAGACATTCAGTGAAGCTTGCCTGATGGTAAGGAAACCAGCCCTAGAACTTCTGCAT TACCTGAAAAACACCAGTTTTGCTTATCCAGCTATACGATATCTTCTGTATGGAGAGAAGGGAACAGGA AAAACCCTAAGTCTTTGCCATGTTATTCATTTCTGTGCAAAACAGGACTGGCTGATACTACATATTCCA GATGCTCATCTTTGGGTGAAAAATTGTCGGGATCTTCTGCAGTCCAGCTACAACAAACAGCGCTTTGAT CAACCTTTAGAGGCTTCAACCTGGCTGAAGAATTTCAAAACTACAAATGAGCGCTTCCTGAACCAGATA AAAGTTCAAGAGAAGTATGTCTGGAATAAGAGAGAAAGCACTGAGAAAGGGAGTCCTCTGGGAGAAGTG GTTGAACAGGGCATAACACGGGTGAGGAACGCCACAGATGCAGTTGGAATTGTGCTGAAAGAGCTAAAG AGGCAAAGTTCTTTGGGTATGTTTCACCTCCTAGTGGCCGTGGATGGAATCAATGCTCTTTGGGGAAGA ACCACTCTGAAAAGAGAAGATAAAAGCCCGATTGCCCCCGAGGAATTAGCACTTGTTCACAACTTGAGG AAAATGATGAAAAATGATTGGCATGGAGGCGCCATTGTGTCGGCTTTGAGCCAGACTGGGTCTCTCTTT AAGCCCCGGAAAGCCTATCTGCCCCAGGAGTTGCTGGGAAAGGAAGGATTTGATGCCCTGGATCCCTTT ATTCCCATCCTGGTTTCCAACTATAACCCAAAGGAATTTGAAAGTTGTATTCAGTATTATTTGGAAAAC AATTGGCTTCAACATGAGAAAGCTCCTACAGAAGAAGGGAAAAAAGAGCTGCTGTTCCTAAGTAACGCG AACCCCTCGCTGCTGGAGCGGCACTGTGCCTACCTCTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001199851
Insert Size	1074 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001199851.1
RefSeq Size	1945 bp
RefSeq ORF	1074 bp
Locus ID	7818
UniProt ID	P51398
Protein Families	Druggable Genome
MW	41 kDa
Gene Summary	Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that also participates in apoptotic pathways which are initiated by tumor necrosis factor-alpha, Fas ligand, and gamma interferon. This protein potentially binds ATP/GTP and might be a functional partner of the mitoribosomal protein S27. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. Pseudogenes corresponding to this gene are found on chromosomes 1q and 2q. [provided by RefSeq, Dec 2010] Transcript Variant: This variant (5) lacks an in-frame exon in the CDS, as compared to variant 2. The resulting isoform (3) lacks an internal segment, as compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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