TNNI3K (FPGT-TNNI3K) (NM_001199327) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CARK; TNNI3K
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC329512 representing NM_001199327. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGCGTGCTGTGCGGCGCGGTCTCAGGGAAGGTGGGGCTATGGCAGCTGCTAGGGACCCTCCGGAAGTA TCGCTGCGAGAAGCCACCCAGCGAAAATTGCGGAGGTTTTCCGAGCTAAGAGGCAAACTTGTAGCACGT GGAGAATTCTGGGACATAGTTGCAATAACAGCGGCTGATGAAAAACAGGAACTTGCTTACAACCAACAG CTGTCAGAAAAGCTGAAAAGAAAGGAGTTACCCCTTGGAGTTCAATATCACGTTTTTGTGGATCCTGCT GGAGCCAAAATTGGAAATGGAGGATCAACACTTTGTGCCCTTCAATGTTTGGAAAAGCTATATGGAGAT AAATGGAATTCTTTTACCATCTTATTAATTCACTCTGATGAATGGAAGAAAAAAGTCAGTGAATCATAT GTTATCACAATAGAAAGATTAGAAGATGACCTGCAGATCAAGGAAAAAGAACTGACAGAACTAAGGAAT ATATTTGGCTCTGATGAAGCCTTCAGTAAAGTCAATTTAAATTACCGCACTGAAAATGGGCTGTCTCTA CTTCATTTATGTTGCATTTGTGGAGGCAAGAAATCACATATTCGAACTCTTATGTTGAAAGGGCTCCGC CCATCTCGACTGACAAGAAATGGATTTACAGCCTTGCATTTAGCAGTTTACAAGGATAATGCAGAATTG ATCACTTCTCTGCTTCACAGTGGAGCTGATATACAGCAGGTTGGATACGGTGGCCTCACTGCCCTCCAT ATTGCTACAATAGCTGGCCACCTAGAGGCTGCTGATGTGCTGTTGCAACATGGAGCTAATGTCAATATT CAAGATGCAGTTTTTTTCACTCCATTGCATATTGCAGCGTACTATGGACATGAACAGGTAACTCGCCTT CTTTTGAAATTTGGTGCTGATGTAAATGTAAGTGGTGAAGTTGGAGATAGACCCCTCCACCTAGCATCT GCAAAAGGATTCTTGAATATTGCAAAACTCTTGATGGAAGAAGGCAGCAAAGCAGATGTGAATGCTCAA GATAATGAAGACCATGTCCCACTCCATTTCTGTTCTCGATTTGGACACCATGATATAGTTAAGTATCTG CTGCAAAGTGATTTGGAAGTTCAACCTCATGTTGTTAATATCTATGGAGATACCCCCTTACACCTGGCA TGCTACAATGGCAAATTTGAAGTTGCCAAGGAAATCATCCAAATATCAGGAACAGAAAGTCTGACTAAG GAAAACATCTTCAGTGAAACAGCTTTTCATAGTGCTTGTACCTATGGCAAGAGCATTGACCTAGTCAAA TTTCTTCTTGATCAGAATGTCATAAACATCAACCACCAAGGAAGGGATGGGCACACTGGATTACACTCT GCTTGCTACCACGGTCACATTCGCCTGGTTCAGTTCTTACTGGATAATGGAGCTGATATGAATCTAGTG GCTTGTGATCCCAGCAGGTCTAGTGGTGAAAAAGATGAGCAGACATGTTTGATGTGGGCTTATGAAAAA GGGCATGATGCCATTGTCACACTCCTGAAGCATTATAAGAGACCACAAGATGAATTGCCCTGTAATGAA TATTCTCAGCCTGGAGGAGATGGCTCCTATGTGTCTGTTCCATCACCCTTGGGGAAGATTAAAAGCATG ACAAAAGAGAAGGCAGATATTCTCCTCCTAAGAGCTGGATTGCCTTCACATTTCCATCTTCAGCTCTCA GAAATTGAGTTCCATGAGATTATTGGCTCAGGTTCTTTTGGGAAAGTATATAAAGGACGATGCAGAAAT AAAATAGTGGCTATAAAACGTTATCGAGCCAATACCTACTGCTCCAAGTCAGATGTGGATATGTTTTGC CGAGAGGTGTCCATTCTCTGCCAGCTCAATCATCCCTGCGTAATTCAGTTTGTGGGTGCTTGCTTGAAT GATCCCAGCCAGTTTGCCATTGTCACTCAATACATATCAGGGGGTTCTCTGTTCTCCCTCCTTCATGAG CAGAAGAGGATTCTTGATTTGCAGTCTAAATTAATTATTGCAGTAGATGTTGCCAAAGGCATGGAGTAC CTTCACAACCTGACACAGCCAATTATACATCGTGACTTGAACAGTCACAATATTCTTCTCTATGAGGAT GGGCATGCTGTGGTGGCAGATTTTGGAGAATCAAGATTTCTACAGTCTCTGGATGAAGACAACATGACA AAACAACCTGGGAACCTCCGTTGGATGGCTCCTGAGGTGTTCACGCAGTGCACTCGGTACACCATCAAA GCAGATGTCTTCAGCTATGCTCTGTGTCTGTGGGAAATTCTCACTGGCGAAATTCCATTCGCTCATCTC AAGCCAGCGGCTGCGGCAGCAGACATGGCTTACCACCACATCAGACCTCCCATTGGCTATTCCATTCCC AAGCCCATATCATCTCTGCTGATACGAGGGTGGAACGCATGTCCTGAAGCAAAATCCAGACCAAGTCAT TACCCAGTCTCATCTGTGTACACAGAAACTCTTAAGAAGAAAAATGAAGATCGTTTTGGGATGTGGATT GAGTATCTCAGAAGATAA
Restriction Sites	SgfI-MluI
ACCN	NM_001199327
Insert Size	2571 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001199327.1
RefSeq Size	2799 bp
RefSeq ORF	2571 bp
Locus ID	100526835
UniProt ID	Q59H18
MW	95.9 kDa
Gene Summary	This locus represents naturally occurring read-through transcription from the neighboring fucose-1-phosphate guanylyltransferase (FPGT) and TNNI3 interacting kinase (TNNI3K) genes. Alternative splicing results in multiple transcript variants that are composed of in-frame exons from each individual gene. [provided by RefSeq, Dec 2010] Transcript Variant: This variant (2) differs in the 3' UTR and coding region, compare to variant 1. The encoded isoform (2) is shorter and has a distinct C-terminus, compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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