KLHL23 (PHOSPHO2-KLHL23) (NM_001199290) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC329504 representing NM_001199290. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGCTCTAAAAGGACAAGAAGATTATATTTATCTTTTCAAGGATTCAACACATCCAGTGGATTTTCTG GATGCATTCAGAACATTTTACTTGGATGGATTATTTACTGATATTACTCTTCAGTGTCCTTCAGGCATA ATTTTCCATTGTCACCGAGCCGTTTTAGCTGCTTGCAGCAATTATTTTAAGGCAATGTTCACAGCTGAC ATGAAAGAAAAATTTAAAAATAAAATAAAACTCTCTGGCATCCACCATGATATTCTGGAAGGCCTTGTA AATTATGCATACACTTCCCAAATTGAAATAACTAAAAGAAATGTTCAAAGCCTGCTTGAGGCAGCGGAT CTGCTACAGTTCCTTTCAGTAAAGAAGGCTTGTGAGCGGTTTTTGGTAAGGCACTTGGATATTGATAAT TGTATTGGAATGCACTCCTTTGCAGAATTTCATGTGTGTCCAGAACTAGAGAAGGAATCTCGAAGAATT CTATGTTCAAAGTTTAAGGAAGTGTGGCAACAAGAAGAATTTCTGGAAATCAGCCTTGAAAAGTTTCTC TTTATCTTGTCCAGAAAGAATCTCAGTGTTTGGAAAGAAGAAGCTATCATAGAGCCAGTTATTAAGTGG ACTGCTCATGATGTAGAAAATCGAATTGAATGCCTCTATAATCTACTGAGCTATATCAACATTGATATA GATCCAGTGTACTTAAAAACAGCCTTAGGCCTTCAAAGAAGCTGCCTGCTCACCGAAAATAAGATCCGC TCCCTAATATACAATGCCTTGAATCCCATGCATAAAGAGATTTCCCAGAGGTCCACAGCCACAATGTAT ATAATTGGAGGCTATTACTGGCATCCTTTATCAGAGGTTCACATATGGGATCCTTTGACAAATGTTTGG ATTCAGGGAGCAGAAATACCAGATTATACCAGGGAGAGCTATGGTGTTACATGTTTAGGACCCAACATT TATGTAACTGGGGGCTACAGGACGGATAACATAGAAGCTCTTGACACAGTGTGGATCTATAACAGTGAA AGTGATGAATGGACAGAAGGTTTGCCAATGCTCAATGCCAGGTATTACCACTGTGCAGTCACCTTGGGT GGCTGTGTCTATGCTTTAGGTGGTTACAGAAAAGGGGCTCCAGCAGAAGAGGCTGAGTTCTATGATCCT TTAAAAGAGAAATGGATTCCTATTGCAAACATGATTAAAGGTGTGGGAAATGCTACTGCCTGTGTCTTA CATGATGTTATCTACGTCATTGGTGGCCACTGTGGCTACAGAGGAAGCTGCACCTATGACAAAGTTCAG AGCTACAATTCCGATATCAACGAATGGAGCCTCATCACCTCCAGTCCACATCCAGAATATGGATTGTGC TCAGTTCCGTTTGAAAATAAGCTCTATCTAGTCGGCGGACAAACTACAATCACAGAATGCTATGACCCT GAACAAAATGAATGGAGAGAGATAGCTCCCATGATGGAAAGGAGGATGGAGTGCGGTGCCGTCATCATG AATGGATGTATTTATGTCACTGGAGGATACTCCTACTCAAAGGGAACGTATCTTCAGAGCATTGAGAAA TATGATCCAGATCTTAATAAGTGGGAAATAGTGGGTAATCTTCCCAGTGCCATGCGGTCTCATGGGTGT GTTTGTGTGTATAATGTCTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001199290
Insert Size	1677 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001199290.1
RefSeq Size	4206 bp
RefSeq ORF	1677 bp
Locus ID	100526832
UniProt ID	Q8NBE8
MW	63.9 kDa
Gene Summary	This locus represents naturally occurring read-through transcription between the neighboring PHOSPHO2 (phosphatase, orphan 2) and KLHL23 (kelch-like 23) genes on chromosome 2. The read-through transcript includes only non-coding PHOSPHO2 exons, and thus encodes the KLHL23 protein. [provided by RefSeq, Dec 2010] Transcript Variant: This variant (1) represents the longer transcript and encodes the protein. Sequence Note: This RefSeq record was created from genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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