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VPRBP (DCAF1) (NM_001171904) Human Untagged Clone
CAT＃: SC329361
VPRBP (untagged)-Human Vpr (HIV-1) binding protein (VPRBP) transcript variant 2
CNY 10,450.00
货期*
8周
规格
Product images
经常一起买 (3)
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | RIP; VPRBP |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_001171904, the custom clone sequence may differ by one or more nucleotides
ATGACTACAGTAGTGGTACATGTGGACTCCAAAGCTGAGCTCACTACCCTGCTGGAGCAG TGGGAAAAGGAACATGGCAGTGGGCAGGACATGGTACCTATCCTTACCAGGATGTCTCAA TTGATTGAAAAAGAAACTGAAGAGTATCGTAAAGGGGATCCAGACCCATTTGATGATCGA CATCCTGGTCGAGCTGATCCAGAGTGTATGCTGGGCCACTTGCTGAGAATACTCTTCAAG AATGATGATTTCATGAATCTGGTGAATGCATATGTGATGACAAGCCGAGAGCCCCCTTTA AACACTGCAGCTTGCAGACTCCTATTAGACATCATGCCAGGGCTGGAAACTGCTGTCGTC TTTCAAGAAAAGGAGGGAATTGTCGAGAATCTTTTCAAATGGGCCCGAGAGGCCGATCAA CCATTGAGGACATATTCTACTGGACTGTTAGGAGGTGCTATGGAAAATCAAGACATTGCT GCCAACTATAGAGATGAAAATTCACAGCTGGTGGCAATAGTGCTTCGAAGACTGAGGGAG CTACAGCTACAGGAAGTGGCTTTGCGGCAGGAAAACAAGCGTCCCAGTCCACGGAAGCTC TCTTCTGAACCCCTTTTGCCTCTGGATGAGGAGGCTGTGGATATGGACTATGGTGACATG GCTGTAGATGTAGTGGATGGAGACCAAGAGGAAGCTTCTGGAGACATGGAGATCTCCTTT CATCTTGATTCAGGCCACAAGACTAGTAGCAGAGTGAACTCAACAACCAAACCTGAGGAT GGAGGATTAAAGAAAAACAAGTCAGCAAAACAGGGTGACAGAGAGAACTTTAGGAAAGCC AAGCAAAAGTTGGGTTTCTCATCTTCTGATCCAGATCGCATGTTTGTTGAGCTGTCTAAT AGCAGTTGGTCAGAAATGTCTCCCTGGGTGATTGGCACCAATTATACCCTTTATCCTATG ACTCCTGCTATCGAGCAGCGACTCATTCTCCAATATTTGACCCCTCTAGGAGAATATCAG GAGCTACTTCCCATATTCATGCAACTTGGATCACGGGAGCTGATGATGTTCTATATTGAC CTGAAGCAAACTAATGATGTCCTGCTTACATTTGAGGCACTAAAGCACCTAGCATCTCTC CTGCTCCATAACAAATTTGCCACAGAATTTGTTGCGCATGGTGGAGTACAGAAATTACTG GAAATACCTCGTCCTTCTATGGCTGCAACCGGTGTGTCTATGTGTTTGTATTACCTATCC TACAATCAGGATGCCATGGAAAGAGTTTGCATGCATCCCCACAATGTTCTGTCTGATGTG GTGAACTATACCCTGTGGTTAATGGAGTGTTCTCATGCTTCAGGATGCTGCCATGCTACC ATGTTTTTTTCAATTTGCTTCTCATTTCGGGCCGTCTTGGAGCTCTTTGACCGCTATGAT GGTCTTCGTCGTCTGGTGAACTTGATCAGTACTTTGGAGATTCTAAATTTGGAAGATCAG GGTGCACTTCTGAGTGATGATGAAATATTTGCTAGCCGCCAAACTGGGAAACATACCTGC ATGGCCTTGCGCAAATACTTTGAGGCTCACCTGGCCATTAAATTGGAACAAGTGAAGCAG TCACTTCAGAGGACTGAGGGTGGCATTCTTGTCCACCCACAACCCCCGTACAAGGCATGC TCATATACTCATGAACAGATTGTGGAAATGATGGAATTTTTGATAGAATATGGCCCAGCG CAGCTATATTGGGAACCAGCTGAAGTTTTCCTCAAACTTTCTTGTGTGCAACTCTTGTTG CAGCTTATTTCTATTGCCTGCAATTGGAAGACCTATTATGCAAGGAATGACACTGTGCGC TTTGCTTTGGATGTCCTGGCTATTCTTACTGTGGTGCCAAAAATCCAGCTCCAGTTGGCA GAATCAGTGGACGTGTTGGATGAGGCTGGATCTACAGTCTCTACTGTAGGTATCAGCATT ATTTTGGGAGTGGCTGAGGGTGAGTTCTTCATCCATGATGCTGAAATTCAGAAGTCAGCA CTTCAGATTATCATCAATTGTGTGTGTGGCCCAGATAACCGAATATCCAGTATTGGTAAA TTTATCTCTGGTACTCCTCGGAGAAAGCTGCCTCAGAACCCTAAAAGCAGTGAGCACACC CTGGCCAAGATGTGGAATGTGGTTCAGTCCAACAACGGCATCAAGGTGCTCCTGTCCTTA CTGTCCATTAAGATGCCCATCACAGATGCAGACCAAATCCGGGCCCTGGCCTGCAAAGCC CTAGTGGGCCTGTCTCGCAGTAGCACTGTCCGGCAGATCATCAGTAAACTGCCCCTTTTC AGCAGCTGCCAGATCCAGCAGCTGATGAAGGAGCCTGTGCTGCAGGACAAGCGCAGTGAC CATGTCAAGTTCTGCAAGTATGCTGCTGAACTCATTGAACGGGTGTCAGGAAAACCACTT CTCATTGGCACTGATGTTTCCCTAGCACGACTGCAGAAAGCAGATGTTGTTGCCCAGTCA AGGATCTCCTTCCCTGAGAAAGAGCTGCTTTTGTTGATACGAAACCATCTTATTTCTAAA GGGCTTGGAGAAACAGCAACCGTGCTGACAAAAGAGGCTGACCTGCCCATGACTGCTGCC TCCCATTCTTCTGCCTTTACCCCAGTCACTGCTGCTGCTTCTCCTGTCTCTCTACCCCGA ACCCCTCGTATCGCTAATGGCATTGCAACTCGTCTGGGCAGCCATGCTGCTGTGGGTGCC TCTGCGCCTTCTGCCCCTACTGCTCATCCTCAGCCACGGCCCCCCCAGGGTCCGCTAGCT CTGCCCGGCCCATCTTATGCAGGCAACTCCCCTTTGATTGGTAGAATCAGTTTTATCAGA GAGAGGCCATCACCCTGCAATGGCAGGAAAATCAGAGTGTTGCGGCAGAAGTCGGACCAT GGTGCCTACAGCCAAAGCCCAGCCATAAAAAAACAGCTGGACAGACATCTTCCTTCCCCA CCTACGCTGGACAGTATAATCACAGAGTATCTTAGAGAACAACATGCTCGCTGCAAGAAT CCAGTTGCCACCTGCCCACCTTTCTCCCTCTTTACTCCTCACCAATGTCCTGAGCCAAAA CAGAGGCGGCAAGCGCCAATAAACTTTACGTCAAGGCTAAACCGCAGGGCATCATTTCCA AAGTATGGAGGGGTGGATGGCGGATGCTTTGATAGGCACCTTATCTTTAGCAGATTCCGT CCTATTTCAGTGTTCCGGGAAGCCAATGAAGATGAGAGTGGCTTCACCTGCTGTGCATTC TCAGCACGGGAGCGGTTCCTGATGCTTGGCACCTGCACAGGGCAGCTGAAGCTCTATAAT GTGTTTAGTGGACAGGAGGAGGCCAGCTATAACTGTCACAACTCAGCCATCACACATCTT GAACCTTCCAGGGATGGGTCCTTGCTGCTGACATCTGCTACTTGGAGCCAGCCTTTGTCT GCACTTTGGGGAATGAAGTCAGTATTTGATATGAAGCATTCCTTCACAGAAGATCACTAT GTTGAGTTCAGTAAGCACTCCCAGGATCGGGTCATCGGCACAAAAGGAGACATTGCCCAC ATTTATGATATTCAGACTGGCAACAAGCTGTTGACTCTGTTTAACCCAGATCTTGCCAAC AACTACAAGAGGAACTGTGCCACCTTTAATCCTACAGATGATCTTGTCTTAAATGATGGC GTCCTCTGGGATGTCCGCTCTGCACAGGCCATCCACAAGTTTGACAAGTTCAATATGAAC ATCAGTGGTGTTTTCCATCCAAATGGACTGGAGGTGATCATTAATACTGAGATTTGGGAC CTTCGAACTTTTCATCTTTTGCATACTGTTCCCGCTCTGGATCAGTGTCGCGTGGTGTTC AATCACACGGGAACAGTGATGTATGGAGCTATGTTGCAGGCAGATGATGAAGATGACTTA ATGGAAGAGAGGATGAAAAGCCCCTTTGGGTCATCCTTCCGAACATTTAATGCAACTGAC TACAAACCTATAGCAACCATTGATGTGAAACGGAACATCTTTGACCTGTGTACAGACACC AAAGACTGCTATCTTGCTGTCATTGAGAATCAAGGCAGCATGGATGCCCTGAACATGGAC ACAGTATGCAGGCTGTATGAAGTGGGCAGGCAGCGTCTGGCAGAGGATGAGGATGAAGAG GAGGACCAGGAAGAGGAAGAACAGGAGGAAGAAGATGATGATGAAGATGATGATGACACC GATGATTTAGATGAGCTTGACACTGACCAGTTGCTGGAGGCGGAGTTGGAGGAGGACGAC AATAATGAGAACGCAGGGGAAGATGGGGACAATGACTTCTCTCCCTCTGATGAGGAGCTA GCAAACCTTCTAGAGGAGGGAGAGGACGGGGAGGATGAAGACTCTGATGCAGATGAGGAG GTGGAACTGATCCTGGGGGACACTGACAGCTCTGACAACTCTGATTTGGAAGATGACATC ATCTTATCTCTGAATGAGTGA |
| Restriction Sites | Please inquire |
| ACCN | NM_001171904 |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001171904.1, NP_001165375.1 |
| RefSeq Size | 5951 bp |
| RefSeq ORF | 4521 bp |
| Locus ID | 9730 |
| UniProt ID | Q9Y4B6 |
| Gene Summary | Acts both as a substrate recognition component of E3 ubiquitin-protein ligase complexes and as an atypical serine/threonine-protein kinase, playing key roles in various processes such as cell cycle, telomerase regulation and histone modification. Probable substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex, named CUL4A-RBX1-DDB1-DCAF1/VPRBP complex, which mediates ubiquitination and proteasome-dependent degradation of proteins such as NF2. Involved in the turnover of methylated proteins: recognizes and binds methylated proteins via its chromo domain, leading to ubiquitination of target proteins by the RBX1-DDB1-DCAF1/VPRBP complex (PubMed:23063525). The CUL4A-RBX1-DDB1-DCAF1/VPRBP complex is also involved in B-cell development: DCAF1 is recruited by RAG1 to ubiquitinate proteins, leading to limit error-prone repair during V(D)J recombination. Also part of the EDVP complex, an E3 ligase complex that mediates ubiquitination of proteins such as TERT, leading to TERT degradation and telomerase inhibition (PubMed:23362280). Also acts as an atypical serine/threonine-protein kinase that specifically mediates phosphorylation of 'Thr-120' of histone H2A (H2AT120ph) in a nucleosomal context, thereby repressing transcription. H2AT120ph is present in the regulatory region of many tumor suppresor genes, down-regulates their transcription and is present at high level in a number of tumors (PubMed:24140421). Involved in JNK-mediated apoptosis during cell competition process via its interaction with LLGL1 and LLGL2 (PubMed:20644714).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an exon in the 5' UTR and uses and alternate in-frame splice site in the 5' coding region, compared to variant 1, resulting in an isoform (2) that is 1 aa shorter than isoform 1. |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC230723 | VPRBP (Myc-DDK-tagged)-Human Vpr (HIV-1) binding protein (VPRBP), transcript variant 2 |
CNY 16,488.00 |
|
| RC230723L3 | Lenti-ORF clone of VPRBP (Myc-DDK-tagged)-Human Vpr (HIV-1) binding protein (VPRBP), transcript variant 2 |
CNY 16,910.00 |
|
| RC230723L4 | Lenti-ORF clone of VPRBP (mGFP-tagged)-Human Vpr (HIV-1) binding protein (VPRBP), transcript variant 2 |
CNY 16,910.00 |
|
| RG230723 | VPRBP (tGFP-tagged) - Human Vpr (HIV-1) binding protein (VPRBP), transcript variant 2 |
CNY 18,088.00 |
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