United States
Germany
Japan
United Kingdom
China
FAM21C (WASHC2C) (NM_001169107) Human Untagged Clone
CAT＃: SC329319
FAM21C (untagged)-Human family with sequence similarity 21 member C (FAM21C) transcript variant 3
CNY 23,940.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | FAM21A; FAM21C; VPEF |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC329319 representing NM_001169107.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGATGAACCGGACGACCCCCGACCAGGAGCTGGTGCCGGCGTCGGAGCCCGTGTGGGAGCGGCCGTGG TCGGTGGAGGAGATCCGCAGGAGCAGCCAGAGCTGGTCGCTGGCGGCCGACGCGGGCCTACTACAGTTT CTACAGGAATTCTCACAGCAAACTATCTCTAGGACCCATGAAATCAAGAAACAAGTGGACGGACTAATC CGGGAAACCAAAGCCACAGATTGTCGCCTGCATAATGTCTTCAATGACTTCCTTATGCTCTCTAATACC CAGTTCATAGAGAATCGTGTATATGATGAAGAAGTGGAGGAGCCAGTACTCAAGGCTGAGGCAGAAAAA ACAGAGCAGGAGAAGACACGAGAGCAGAAAGAAGTAGATCTCATTCCTAAAGTCCAGGAGGCTGTGAAC TATGGCTTACAAGTATTGGACAGTGCCTTTGAGCAACTTGATATCAAAGCAGGAAACTCAGACTCCGAG GAAGATGATGCTAATGGGCGGGTGGAACTGATCCTTGAACCAAAGGATCTATACATTGATCGTCCTTTA CCATATCTCATTGGGTCAAAGCTGTTCATGGAACAAGAAGATGTAGGTCTTGGAGAGCTGTCCAGTGAA GAAGGCTCTGTAGGCAGTGATCGTGGCAGTATTGTGGACACTGAGGAAGAGAAAGAAGAGGAGGAGTCA GATGAAGATTTTGCCCATCACAGTGACAATGAACAAAACCAGCACACCACACAAATGAGTGATGAGGAA GAGGATGATGATGGCTGTGACCTTTTTGCTGACTCTGAGAAGGAGGAGGAAGATATTGAGGACATTGAA GAAAATACTAGACCTAAAAGAAGCAGACCTACATCGTTTGCAGATGAGCTGGCTGCCCGCATCAAGGGG GATGCCATGGGTCGAGTGGACGAGGAGCCGACAAATGAAGAGGATAACTTATTCGCACCCCCCAAGCTG ACCGACGAGGACTTCTCGCCATTTGGCTCTGGAGGTGGCCTGTTCAGTGGCGGCAAGGGGCTATTTGAT GATGAGGACGAGGAGAGTGACCTCTTCACGGAAGCCTCCCAGGATCGGCAAGCTGGAGCCTCTGTTAAG GAGGAGTCTTCATCATCCAAACCTGGAAAGAAAATCCCAGCAGGAGCTGTTTCTGTATTTTTAGGAGAC ACGGATGTGTTTGGTGCTGCCTCCGTTCCATCACTGAAGGAGCCACAGAAGCCTGAGCAGCCCACTCCA AGGAAAAGCCCCTATGGTCCCCCTCCCACTGGCCTCTTTGATGATGATGATGGTGATGATGATGACGAC TTTTTCTCGGCACCCCACAGCAAACCTTCTAAAACACGCAAAGTCCAATCCACTGCCGATATCTTTGGT GACGAAGAAGGAGATCTGTTCAAAGAAAAAGCCGTAGCATCGCCAGAAGCCACTGTGAGTCAGACAGAT GAAAATAAAGCAAGAGCAGAAAAAAAGGTTACCTTATCTTACAGCAAAAATCTCAAGCCCTCATCAGAA ACAAAGACTCAAAAAGGCTTATTTTCAGATGAGGAGGACTCTGAGGATTTGTTTTCTTCTCAAAGTGCG AGTAACTTAAAAGGTGCATCTCTGCTGCCTGGCAAGCTCCCCACGTCGGTTTCCCTGTTTGATGATGAA GATGAAGAGGATAATCTTTTTGGGGGTACAGCTGCTAAGAAGCAGACATTGTCTCTACAAGCTCAGAGA GAAGAGAAAGCAAAAGCCTCCGAGCTCTCCAAAAAGAAAGCATCTGCCCTGTTGTTCAGCAGTGATGAG GAGGACCAGTGGAATATTCCTGCTTCACAGACCCACTTAGCATCTGACAGCAGGTCTAAAGGAGAACCC AGGGATTCTGGGACCCTCCAGAGCCAGGAGGCCAAGGCTGTGAAAAAGACCAGTCTCTTTGAGGAAGAC AAAGAAGATGATCTTTTTGCCATTGCCAAGGACAGCCAAAAGAAGACCCAGAGAGTGTCACTCCTCTTT GAAGACGATGTTGATAGCGGAGGCTCTCTGTTTGGCTCTCCTCCCACATCTGTTCCTCCTGCAACAAAG GAAGGACTTTTGACTAGATCTGCTCAGGAAACAGTCAAGCATTCTGATTTATTTTCTTCATCATCCCCA TGGGACAAAGGAACCAAGCCTAGAACCAAAACTGTTCTTAGCTTGTTTGATGAGGAAGAGGATAAAATG GAAGATCAAAACATTATCCAGGCTCCACAGAAAGAAGTAGGAAAGGGCTGCGATCCTGATGCCCACCCC AAGAGCACAGGTGTCTTCCAGGATGAAGAGCTGCTTTTCAGCCACAAGCTCCAAAAGGACAATGACCCA GATGTTGACCTTTTTGCTGGCACCAAAAAAACCAAGCTGTTAGAGCCAAGTGTTGGGAGCCTGTTTGGG GATGATGAAGATGATGATCTTTTCAGCTCTGCCAAGTCCCAGCCTTTGGTACAAGAGAAAAAGAGAGTA GTGAAAAAAGACCACTCTGTTAACTCTTTCAAAAACCAGAAACATCCTGAATCCATTCAAGGTAGTAAA GAAAAAGGCATATGGAAGCCGGAAACACCTCAGGCAAATTTAGCGATCAACCCAGCGGCCTTGCTGCCC ACAGCGGCTTCCCAGATCTCTGAAGTAAAGCCTGTTTTGCCAGAATTGGCTTTTCCTTCATCTGAACAC AGAAGGAGCCACGGTCTGGAAAGTGTGCCTGTCCTTCCCGGGAGTGGGGAGGCCGGTGTGAGTTTTGAT CTTCCAGCTCAGGCAGACACCTTACACAGTGCAAACAAGAGCCGTGTCAAGATGAGAGGGAAGCGTAGA CCGCAGACCCGTGCAGCTAGGCGGCTGGCTGCTCAGGAGTCCAGCGAGGCTGAGGACATGAGCGTCCCC AGAGGACCCATTGCACAGTGGGCTGATGGCGCCATTTCCCCAAATGGCCATCGGCCACAGCTCAGAGCA GCCAGTGGAGAAGACAGCACTGAGGAGGCCCTGGCAGCTGCCGCTGCACCTTGGGAAGGTGGTCCTGTG CCTGGAGTGGACACAAGCCCCTTTGCAAAGTCTCTGGGTCATTCCAGAGGGGAGGCTGACCTTTTTGAT TCTGGGGACATTTTTTCCACGGGCACTGGATCTCAGTCCGTGGAGAGAACAAAACCCAAGGCAAAGATA GCAGAGAATCCTGCCAACCCACCAGTGGGTGGTAAAGCAAAGAGCCCCATGTTTCCTGCTCTAGGCGAG GCCAGCAGTGATGATGATCTCTTTCAGTCTGCTAAACCAAAACCAGCAAAGAAAACAAATCCCTTTCCT CTCCTGGAAGATGAGGATGACCTCTTTACAGATCAGAAAGTCAAGAAGAATGAGACAAAATCCAGTAGT CAGCAGGATGTCATATTAACAACACAAGATATTTTTGAGGATGATATATTTGCTACGGAAGCAATTAAA CCCTCTCAGAAAACCAGAGAGAAGGAGAAAACATTGGAATCTAATTTATTTGATGATAACATTGATATC TTTGCTGACTTAACTGTAAAACCAAAAGAAAAGTCCAAAAAGAAAGTGGAAGCCAAGTCTATATTTGAT GATGATATGGATGACATCTTCTCCACTGGTATCCAGGCTAAGACAACCAAACCAAAAAGCCGATCTGCA CAGGCCGCACCTGAACCAAGATTTGAACACAAGGTGTCCAACATCTTTGATGATCCCCTGAATGCCTTT GGAGGCCAGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001169107 |
| Insert Size | 3738 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001169107.1 |
| RefSeq Size | 4429 bp |
| RefSeq ORF | 3738 bp |
| Locus ID | 253725 |
| UniProt ID | Q9Y4E1 |
| MW | 136.5 kDa |
| Gene Summary | Acts at least in part as component of the WASH core complex whose assembly at the surface of endosomes inhibits WASH nucleation-promoting factor (NPF) activity in recruiting and activating the Arp2/3 complex to induce actin polymerization and is involved in the fission of tubules that serve as transport intermediates during endosome sorting. Mediates the recruitment of the WASH core complex to endosome membranes via binding to phospholipids and VPS35 of the retromer CSC. Mediates the recruitment of the F-actin-capping protein dimer to the WASH core complex probably promoting localized F-actin polymerization needed for vesicle scission (PubMed:19922874, PubMed:20498093, PubMed:22513087, PubMed:23331060). Via its C-terminus binds various phospholipids, most strongly phosphatidylinositol 4-phosphate (PtdIns-(4)P), phosphatidylinositol 5-phosphate (PtdIns-(5)P) and phosphatidylinositol 3,5-bisphosphate (PtdIns-(3,5)P2). Involved in the endosome-to-plasma membrane trafficking and recycling of SNX27-retromer-dependent cargo proteins, such as GLUT1 (PubMed:25278552). Required for the association of DNAJC13, ENTR1, ANKRD50 with retromer CSC subunit VPS35 (PubMed:24980502). Required for the endosomal recruitment of CCC and retriever complexes subunits COMMD1 and CCDC93 as well as the retrievere complex subunit VPS35L (PubMed:25355947, PubMed:28892079).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) lacks two alternate in-frame exons in the central coding region, compared to variant 1, resulting in an isoform (3) that is shorter than isoform 1. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC230681 | FAM21C (Myc-DDK-tagged)-Human family with sequence similarity 21, member C (FAM21C), transcript variant 3 |
CNY 8,672.00 |
|
| RC230681L3 | Lenti ORF clone of Human family with sequence similarity 21, member C (FAM21C), transcript variant 3, Myc-DDK-tagged |
CNY 14,350.00 |
|
| RC230681L4 | Lenti ORF clone of Human family with sequence similarity 21, member C (FAM21C), transcript variant 3, mGFP tagged |
CNY 14,350.00 |
|
| RG230681 | FAM21C (tGFP-tagged) - Human family with sequence similarity 21, member C (FAM21C), transcript variant 3 |
CNY 13,680.00 |
