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PHKA1 (NM_001172436) Human Untagged Clone
CAT＃: SC329301
PHKA1 (untagged)-Human phosphorylase kinase alpha 1 (muscle) (PHKA1) transcript variant 3
CNY 10,450.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | PHKA |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_001172436, the custom clone sequence may differ by one or more nucleotides
ATGAGGAGCCGGAGTAACTCCGGGGTCCGGCTGGACGGCTACGCTCGACTGGTGCAACAG ACCATCCTGTGCCATCAGAATCCAGTGACTGGCTTGCTTCCAGCCAGCTATGATCAGAAA GATGCTTGGGTCCGAGATAATGTGTACAGCATCTTGGCTGTGTGGGGTTTGGGCCTGGCC TATCGGAAGAATGCAGACCGGGATGAGGATAAGGCAAAGGCCTATGAATTGGAGCAGAGT GTAGTGAAGCTGATGAGAGGACTACTGCACTGCATGATCAGACAGGTGGATAAAGTAGAA TCCTTCAAATATAGTCAGAGTACTAAGGATAGCCTCCATGCAAAGTACAACACCAAAACC TGTGCCACTGTAGTGGGTGATGATCAATGGGGACACCTGCAGTTGGATGCTACCTCTGTG TACCTGCTCTTCTTAGCCCAAATGACTGCCTCAGGACTCCATATCATCCACAGCCTAGAT GAAGTCAATTTCATACAGAACCTTGTGTTTTACATTGAAGCTGCATATAAAACTGCTGAC TTCGGGATATGGGAACGTGGAGACAAGACCAACCAAGGGATCTCAGAGTTGAATGCCAGT TCAGTTGGAATGGCAAAGGCAGCCCTGGAAGCATTAGATGAACTGGATCTGTTTGGTGTG AAAGGTGGGCCTCAATCAGTTATCCATGTCCTGGCTGATGAAGTACAGCACTGCCAGTCT ATCCTAAATTCACTACTGCCCCGTGCTTCAACATCAAAAGAGGTTGATGCTAGTCTACTC TCAGTGGTTTCCTTCCCTGCCTTTGCAGTAGAGGATAGCCAGTTGGTGGAGCTCACAAAA CAGGAAATCATCACCAAGCTTCAGGGTCGTTATGGTTGCTGTCGCTTTCTACGAGATGGA TATAAAACTCCTAAAGAGGATCCCAATCGTCTGTACTATGAACCAGCTGAGCTGAAGCTA TTTGAAAACATTGAGTGTGAATGGCCATTGTTCTGGACATACTTTATTCTTGATGGGGTC TTCAGTGGCAATGCAGAACAGGTTCAAGAATATAAAGAGGCTCTTGAAGCAGTCCTCATC AAGGGCAAAAATGGAGTCCCACTTCTGCCAGAGCTGTACAGTGTTCCTCCTGACAGGGTC GATGAAGAATATCAGAATCCTCACACTGTGGACCGAGTCCCCATGGGGAAATTGCCTCAC ATGTGGGGTCAGTCTCTATACATTTTAGGAAGCTTGATGGCAGAGGGATTTTTAGCCCCT GGAGAAATTGATCCCCTGAATCGCAGGTTTTCTACTGTACCGAAGCCCGATGTTGTGGTT CAAGTCTCCATTCTAGCTGAAACAGAAGAAATCAAGACCATTTTGAAGGACAAGGGAATT TACGTGGAGACCATTGCTGAGGTATACCCCATCAGAGTACAACCAGCTCGTATTCTCAGC CACATTTATTCCAGCCTAGGATGCAACAATAGAATGAAACTCAGTGGACGACCCTACAGA CACATGGGAGTGCTTGGAACTTCAAAACTCTATGACATTCGGAAAACTATCTTTACTTTC ACTCCACAGTTTATAGACCAGCAACAGTTCTACCTGGCTCTGGACAACAAGATGATAGTG GAAATGCTTAGAACAGACCTCTCCTACCTCTGTAGCCGCTGGCGGATGACAGGCCAGCCC ACCATCACCTTCCCCATCTCACACAGCATGCTTGATGAAGATGGAACAAGCTTGAATTCA AGTATCCTGGCAGCACTCCGAAAAATGCAAGATGGGTATTTTGGTGGGGCAAGGGTTCAA ACAGGTAAATTGTCAGAGTTTTTGACAACATCTTGTTGCACACACTTGAGCTTCATGGAC CCTGGACCTGAGGGTAAGCTGTACAGTGAAGATTATGATGACAACTATGATTACCTGGAA TCTGGCAACTGGATGAATGATTATGATTCAACCAGTCATGATGTTCACATGTATCTTCCT ACGAAGTTATTTCAGGCTTCCCGGCCTTCATTCAACTTACTTGATTCACCTCATCCCCGA CAGGAGAACCAGGTTCCCTCTGTTCGTGTAGAAATACATCTTCCTAGAGACCAGTCTGGG GAGGTGGACTTTAAAGCACTGGTTTTACAGTTGAAGGAGACCTCAAGCTTACAGGAACAA GCTGATATCCTCTATATGCTGTATACTATGAAAGGACCTGACTGGAACACTGAATTGTAT AATGAACGGAGTGCTACAGTGAGAGAGCTTCTTACCGAGCTGTATGGCAAAGTGGGAGAA ATTCGTCACTGGGGCCTGATCCGATACATTTCTGGGATCTTAAGGAAGAAAGTGGAAGCA CTTGATGAGGCCTGCACAGACCTTCTCTCCCACCAGAAACATTTGACAGTAGGACTTCCT CCAGAACCTCGAGAAAAGACTATCTCTGCACCTCTGCCCTATGAGGCGCTCACTCAGCTG ATAGATGAAGCCAGTGAAGGGGATATGAGCATTTCAATCCTTACACAGGAAATAATGGTA TATCTAGCCATGTATATGCGAACCCAGCCTGGCCTCTTTGCTGAAATGTTTCGACTTCGA ATTGGTCTGATCATACAAGTTATGGCAACAGAACTGGCCCACTCCCTTCGATGCTCAGCT GAGGAAGCCACAGAGGGCCTGATGAATCTCAGTCCTTCGGCCATGAAGAATCTCCTGCAT CACATTCTCAGCGGCAAGGAGTTTGGAGTGGAACGAAGCGTTCGTCCCACTGATTCAAAT GTCAGTCCTGCTATTTCTATCCACGAGATTGGTGCTGTTGGAGCAACCAAAACAGAACGA ACTGGGATCATGCAGTTAAAAAGTGAGATAAAGCAGTCACCTGGAACCTCTATGACTCCA AGTAGTGGGTCCTTTCCTAGTGCATATGATCAGCAGTCATCTAAAGATAGTCGTCAAGGT CAATGGCAACGCCGAAGAAGGCTGGATGGGGCACTGAATAGAGTTCCAGTTGGATTTTAT CAGAAAGTATGGAAAGTTTTGCAGAAGTGTCACGGACTTTCTGTTGAAGGGTTTGTCCTT CCTTCCTCTACCACTAGAGAGATGACTCCAGGTGAGATTAAATTCTCTGTTCATGTGGAG TCTGTCCTGAATCGTGTACCTCAGCCAGAGTACCGTCAGCTGCTGGTTGAAGCCATCCTT GTCCTCACCATGCTGGCAGATATTGAAATTCATAGCATCGGAAGCATCATTGCTGTGGAA AAAATAGTGCATATTGCCAATGACTTGTTCCTTCAAGAACAGAAAACCCTTGGCGCAGAT GATACCATGTTGGCAAAGGATCCCGCATCTGGCATCTGTACTCTTCTGTATGACAGTGCA CCCAGTGGCAGGTTTGGCACCATGACCTACCTCTCCAAGGCAGCCGCCACCTACGTGCAG GAGTTCCTGCCCCACAGCATCTGTGCCATGCAATGA |
| Restriction Sites | Please inquire |
| ACCN | NM_001172436 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001172436.1, NP_001165907.1 |
| RefSeq Size | 5961 bp |
| RefSeq ORF | 3456 bp |
| Locus ID | 5255 |
| UniProt ID | P46020 |
| Protein Families | Druggable Genome |
| Protein Pathways | Calcium signaling pathway, Insulin signaling pathway |
| Gene Summary | Phosphorylase kinase is a polymer of 16 subunits, four each of alpha, beta, gamma and delta. The alpha subunit includes the skeletal muscle and hepatic isoforms, and the skeletal muscle isoform is encoded by this gene. The beta subunit is the same in both the muscle and hepatic isoforms, and encoded by one gene. The gamma subunit also includes the skeletal muscle and hepatic isoforms, which are encoded by two different genes. The delta subunit is a calmodulin and can be encoded by three different genes. The gamma subunits contain the active site of the enzyme, whereas the alpha and beta subunits have regulatory functions controlled by phosphorylation. The delta subunit mediates the dependence of the enzyme on calcium concentration. Mutations in this gene cause glycogen storage disease type 9D, also known as X-linked muscle glycogenosis. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene. A pseudogene has been found on chromosome 1.[provided by RefSeq, Feb 2010] Transcript Variant: This variant (3) lacks two alternate in-frame exons in the middle and 3' coding regions, respectively, compared to variant 1, resulting in a shorter protein (isoform 3), compared to isoform 1. |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC230663 | PHKA1 (Myc-DDK-tagged)-Human phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant 3 |
CNY 11,500.00 |
|
| RC230663L3 | Lenti-ORF clone of PHKA1 (Myc-DDK-tagged)-Human phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant 3 |
CNY 13,400.00 |
|
| RC230663L4 | Lenti-ORF clone of PHKA1 (mGFP-tagged)-Human phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant 3 |
CNY 13,400.00 |
|
| RG230663 | PHKA1 (tGFP-tagged) - Human phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant 3 |
CNY 12,640.00 |
