United States
Germany
Japan
United Kingdom
China
MAN2B1 (NM_001173498) Human Untagged Clone
CAT＃: SC329254
MAN2B1 (untagged)-Human mannosidase alpha class 2B member 1 (MAN2B1) transcript variant 2
CNY 10,450.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | LAMAN; MANB |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_001173498, the custom clone sequence may differ by one or more nucleotides
ATGGGCGCCTACGCGCGGGCTTCGGGGGTCTGCGCTCGCGGCTGCCTGGACTCAGCAGGC CCCTGGACCATGTCCCGCGCCCTGCGGCCACCGCTCCCGCCTCTCTGCTTTTTCCTTTTG TTGCTGGCGGCTGCCGGTGCTCGGGCCGGGGGATACGAGACATGCCCCACAGTGCAGCCG AACATGCTGAACGTGCACCTGCTGCCTCACACACATGATGACGTGGGCTGGCTCAAAACC GTGGACCAGTACTTTTATGGAATCAAGAATGACATCCAGCACGCCGGTGTGCAGTACATC CTGGACTCGGTCATCTCTGCCTTGCTGGCAGATCCCACCCGTCGCTTCATTTACGTGGAG ATTGCCTTCTTCTCCCGTTGGTGGCACCAGCAGACAAATGCCACACAGGAAGTCGTGCGA GACCTTGTGCGCCAGGGGCGCCTGGAGTTCGCCAATGGTGGCTGGGTGATGAACGATGAG GCAGCCACCCACTACGGTGCCATCGTGGACCAGATGACACTTGGGCTGCGCTTTCTGGAG GACACATTTGGCAATGATGGGCGACCCCGTGTGGCCTGGCACATTGACCCCTTCGGCCAC TCTCGGGAGCAGGCCTCGCTGTTTGCGCAGATGGGCTTCGACGGCTTCTTCTTTGGGCGC CTTGATTATCAAGATAAGTGGGTACGGATGCAGAAGCTGGAGATGGAGCAGGTGTGGCGG GCCAGCACCAGCCTGAAGCCCCCGACCGCGGACCTCTTCACTGGTGTGCTTCCCAATGGT TACAACCCGCCAAGGAATCTGTGCTGGGATGTGCTGTGTGTCGATCAGCCGCTGGTGGAG GACCCTCGCAGCCCCGAGTACAACGCCAAGGAGCTGGTCGATTACTTCCTAAATGTGGCC ACTGCCCAGGGCCGGTATTACCGCACCAACCACACTGTGATGACCATGGGCTCGGACTTC CAATATGAGAATGCCAACATGTGGTTCAAGAACCTTGACAAGCTCATCCGGCTGGTAAAT GCGCAGGCAAAAGGAAGCAGTGTCCATGTTCTCTACTCCACCCCCGCTTGTTACCTCTGG GAGCTGAACAAGGCCAACCTCACCTGGTCAGTGAAACATGACGACTTCTTCCCTTACGCG GATGGCCCCCACCAGTTCTGGACCGGTTACTTTTCCAGTCGGCCGGCCCTCAAACGCTAC GAGCGCCTCAGCTACAACTTCCTGCAGGTGTGCAACCAGCTGGAGGCGCTGGTGGGCCTG GCGGCCAACGTGGGACCCTATGGCTCCGGAGACAGTGCACCCCTCAATGAGGCGATGGCT GTGCTCCAGCATCACGACGCCGTCAGCGGCACCTCCCGCCAGCACGTGGCCAACGACTAC GCGCGCCAGCTTGCGGCAGGCTGGGGGCCTTGCGAGGTTCTTCTGAGCAACGCGCTGGCG CGGCTCAGAGGCTTCAAAGATCACTTCACCTTTTGCCAACAGCTAAACATCAGCATCTGC CCGCTCAGCCAGACGGCGGCGCGCTTCCAGGTCATCGTTTATAATCCCCTGGGGCGGAAG GTGAATTGGATGGTACGGCTGCCGGTCAGCGAAGGCGTTTTCGTTGTGAAGGACCCCAAT GGCAGGACAGTGCCCAGCGATGTGGTAATATTTCCCAGCTCAGACAGCCAGGCGCACCCT CCGGAGCTGCTGTTCTCAGCCTCACTGCCCGCCCTGGGCTTCAGCACCTATTCAGTAGCC CAGGTGCCTCGCTGGAAGCCCCAGGCCCGCGCACCACAGCCCATCCCCAGAAGATCCTGG TCCCCTGCTTTAACCATCGAAAATGAGCACATCCGGGCAACGTTTGATCCTGACACAGGG CTGTTGATGGAGATTATGAACATGAATCAGCAACTCCTGCTGCCTGTTCGCCAGACCTTC TTCTGGTACAACGCCAGTATAGGTGACAACGAAAGTGACCAGGCCTCAGGTGCCTACATC TTCAGACCCAACCAACAGAAACCGCTGCCTGTGAGCCGCTGGGCTCAGATCCACCTGGTG AAGACACCCTTGGTGCAGGAGGTGCACCAGAACTTCTCAGCTTGGTGTTCCCAGGTGGTT CGCCTGTACCCAGGACAGCGGCACCTGGAGCTAGAGTGGTCGGTGGGGCCGATACCTGTG GGCGACACCTGGGGGAAGGAGGTCATCAGCCGTTTTGACACACCGCTGGAGACAAAGGGA CGCTTCTACACAGACAGCAATGGCCGGGAGATCCTGGAGAGGAGGCGGGATTATCGACCC ACCTGGAAACTGAACCAGACGGAGCCCGTGGCAGGAAACTACTATCCAGTCAACACCCGG ATTTACATCACGGATGGAAACATGCAGCTGACTGTGCTGACTGACCGCTCCCAGGGGGGC AGCAGCCTGAGAGATGGCTCGCTGGAGCTCATGGTGCACCGAAGGCTGCTGAAGGACGAT GGACGCGGAGTATCGGAGCCACTAATGGAGAACGGGTCGGGGGCGTGGGTGCGAGGGCGC CACCTGGTGCTGCTGGACACAGCCCAGGCTGCAGCCGCCGGACACCGGCTCCTGGCGGAG CAGGAGGTCCTGGCCCCTCAGGTGGTGCTGGCCCCGGGTGGCGGCGCCGCCTACAATCTC GGGGCTCCTCCGCGCACGCAGTTCTCAGGGCTGCGCAGGGACCTGCCGCCCTCGGTGCAC CTGCTCACGCTGGCCAGCTGGGGCCCCGAAATGGTGCTGCTGCGCTTGGAGCACCAGTTT GCCGTAGGAGAGGATTCCGGACGTAACCTGAGCGCCCCCGTTACCTTGAACTTGAGGGAC CTGTTCTCCACCTTCACCATCACCCGCCTGCAGGAGACCACGCTGGTGGCCAACCAGCTC CGCGAGGCAGCCTCCAGGCTCAAGTGGACAACAAACACAGGCCCCACACCCCACCAAACT CCGTACCAGCTGGACCCGGCCAACATCACGCTGGAACCCATGGAAATCCGCACTTTCCTG GCCTCAGTTCAATGGAAGGAGGTGGATGGTTAG |
| Restriction Sites | Please inquire |
| ACCN | NM_001173498 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001173498.1, NP_001166969.1 |
| RefSeq Size | 3239 bp |
| RefSeq ORF | 3033 bp |
| Locus ID | 4125 |
| UniProt ID | O00754 |
| Protein Families | Druggable Genome |
| Protein Pathways | Lysosome, Other glycan degradation |
| Gene Summary | This gene encodes an enzyme that hydrolyzes terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides. Its activity is necessary for the catabolism of N-linked carbohydrates released during glycoprotein turnover and it is member of family 38 of glycosyl hydrolases. The full length protein is processed in two steps. First, a 49 aa leader sequence is cleaved off and the remainder of the protein is processed into 3 peptides of 70 kDa, 42 kDa (D) and 13/15 kDa (E). Next, the 70 kDa peptide is further processed into three peptides (A, B and C). The A, B and C peptides are disulfide-linked. Defects in this gene have been associated with lysosomal alpha-mannosidosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2010] Transcript Variant: This variant (2) lacks a CAG copy at an splice site in the CDS, as compared to variant 1. The resulting isoform (2) lacks an internal Q, as compared to isoform 1. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC230616 | MAN2B1 (Myc-DDK-tagged)-Human mannosidase, alpha, class 2B, member 1 (MAN2B1), transcript variant 2 |
CNY 10,070.00 |
|
| RC230616L3 | Lenti-ORF clone of MAN2B1 (Myc-DDK-tagged)-Human mannosidase, alpha, class 2B, member 1 (MAN2B1), transcript variant 2 |
CNY 11,970.00 |
|
| RC230616L4 | Lenti-ORF clone of MAN2B1 (mGFP-tagged)-Human mannosidase, alpha, class 2B, member 1 (MAN2B1), transcript variant 2 |
CNY 11,970.00 |
|
| RG230616 | MAN2B1 (tGFP-tagged) - Human mannosidase, alpha, class 2B, member 1 (MAN2B1), transcript variant 2 |
CNY 11,120.00 |
