GGA1 (NM_001172688) Human Untagged Clone

CAT#: SC328922

GGA1 (untagged)-Human golgi-associated gamma adaptin ear containing ARF binding protein 1 (GGA1) transcript variant 5



  "NM_001172688" in other vectors (4)

CNY 9,120.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


Rabbit polyclonal anti-GGA-1 antibody
    • 100 ug

CNY 8,025.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>SC328922 representing NM_001172688.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGAAGAGCTGCGGCAAGCGGTTCCACGACGAAGTGGGCAAGTTCCGCTTTCTCAACGAGCTCATCAAG
GTCGTGTCTCCCAAGTATCTGGGCTCTCGGACATCGGAGAAGGTGAAGAACAAGATCTTGGAGCTCCTC
TACAGCTGGACAGTGGGCCTGCCCGAGGAGGTGAAAATCGCAGAGGCCTACCAGATGCTAAAGAAGCAG
GGGATTGTAAAGTCCGACCCCAAGCTTCCAGATGACACTACCTTTCCCCTTCCTCCTCCACGGCCGAAG
AATGTGATCTTTGAAGATGAGGAGAAATCCAAGATGCTGGCCCGCCTGCTGAAGAGCTCCCATCCCGAA
GACCTCCGCGCAGCCAATAAGCTCATCAAAGAGATGGTGCAGGAGGACCAGAAGCGGATGGAGAAGATC
TCGAAGAGGGTGAATGCCATCGAGGAGGTGAACAACAATGTGAAACTGCTCACGGAGATGGTGATGAGC
CACAGCCAGGGCGGCGCAGCAGCTGGCAGCAGCGAGGACCTCATGAAGGAACTGTACCAGCGCTGTGAG
CGGATGCGGCCCACGCTCTTCCGACTGGCGAGTGACACAGAGGACAATGATGAGGCCTTAGCGGAGATC
CTGCAGGCCAATGACAACCTCACCCAGGTGATCAACCTGTATAAGCAGCTGGTGCGGGGTGAGGAGGTC
AACGGTGATGCCACAGCCGGCTCCATCCCTGGGAGCACCTCGGCCCTGCTGGATCTCTCAGGCCTGGAT
CTCCCGCCTGCGGGCACCACCTACCCAGCTATGCCCACCCGCCCTGGCGAGCAGGCCAGCCCTGAGCAG
CCCAGTGCCTCAGTTTCCCTGCTTGACGACGAGCTCATGTCTCTGGGCCTCAGTGACCCCACACCCCCT
TCAGGCCCAAGCCTGGATGGTACCGGATGGAACAGCTTCCAGTCGTCGGATGCCACTGAGCCCCCAGCC
CCTGCTCTGGCCCAGGCCCCCAGTATGGAAAGCCGACCCCCAGCGCAGACATCCCTGCCAGCAAGCAGC
GGTCTGGACGACCTAGACCTCCTGGGGAAGACCCTCCTGCAGCAGTCGCTGCCCCCGGAATCCCAGCAA
GTGCGGTGGGAGAAGCAGCAGCCAACCCCCCGGCTCACACTCCGGGACCTGCAGAATAAGAGCAGCAGC
TGCAGCTCCCCCAGCTCCAGCGCCACCAGCCTTCTCCACACCGTGTCCCCAGAGCCCCCCAGGCCTCCG
CAGCAGCCCGTACCAACCGAGCTCTCACTGGCCAGCATCACTGTGCCCCTGGAGTCCATCAAACCCAGC
AACATCCTGCCCGTGACTGTGTATGACCAGCACGGCTTCCGCATCCTCTTCCATTTTGCCCGGGACCCA
CTGCCAGGGCGCTCCGACGTGCTGGTGGTGGTGGTTTCCATGCTGAGCACCGCCCCCCAGCCCATCCGC
AACATCGTGTTCCAGTCAGCTGTCCCCAAGGTTATGAAGGTGAAGCTGCAGCCACCCTCGGGCACGGAG
CTGCCAGCTTTTAACCCCATCGTCCACCCCTCAGCAATCACCCAGGTCCTGCTGCTTGCCAACCCCCAG
AAGGAGAAGGTTCGCCTCCGCTACAAGCTCACCTTCACCATGGGTGACCAGACCTACAACGAGATGGGG
GATGTGGACCAGTTCCCCCCACCTGAAACCTGGGGTAGCCTCTAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI     
ACCN NM_001172688
Insert Size 1701 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001172688.1
RefSeq Size 3231 bp
RefSeq ORF 1701 bp
Locus ID 26088
UniProt ID Q9UJY5
Protein Families Druggable Genome
Protein Pathways Lysosome
MW 62.1 kDa
Gene Summary This gene encodes a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding (GGA) protein family. Members of this family are ubiquitous coat proteins that regulate the trafficking of proteins between the trans-Golgi network and the lysosome. These proteins share an amino-terminal VHS domain which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Transcript Variant: This variant (5) has an alternate 5' sequence, which results in a downstream AUG start codon, as compared to variant 1. The resulting isoform (5) has a shorter N-terminus, as compared to isoform 1.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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