EML6 (NM_001039753) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC328094 representing NM_001039753. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCGGATCGGACGGCGCCCCGCTGCCAGCTCCGGCTGGAGTGGGTGTACGGGTACCGGGGTCACCAG TGCCGCAACAACCTGTACTACACGGCAGGCAAGGAGGTGGTCTACTTTGTGGCTGGGGTCGGGGTGGTT TACAACACCCGCGAGCACAGCCAAAAATTCTTCCTGGGACACAACGACGACATTATCAGCCTTGCCTTA CACCCAGACAAAACTCTCGTTGCAACTGGCCAAGTCGGGAAGGAGCCATATATATGCATATGGGATTCC TATAATGTCCAGACTGTGTCTCTTCTTAAAGATGTCCATACACATGGAGTTGCCTGCCTGGCTTTTGAC TCAGATGGACAGCGTTTAGCCTCTGTGGGGTTGGATGCCAAAAACACAGTCTGCATTTGGGACTGGAGG AAGGGAAAACTTCTGGCGTCAGCCACCGGCCATTCTGACAGGATTTTTGATATTTCCTGGGATCCATAT CAGCCAAACAGAGTGGTTAGCTGTGGAGTAAAACACATAAAGTTTTGGACACTGTGTGGAAATGCCCTG ACTGCAAAAAGAGGGATATTTGGCAAAACAGGGGATCTTCAGACCATCCTTTGCCTTGCATGTGCCAAA GAAGACATCACCTACTCTGGTGCTTTAAATGGTGACATCTATGTCTGGAAAGGGCTCAATTTAGTCCGC ACCATTCAAGGAGCACATAGTGCTGGAATCTTTAGCATGTATGCTTGTGAAGAAGGCTTTGCCACTGGT GGGCGAGATGGGTGTATACGACTGTGGGACACTGATTTCAAACCAATAACCAAAATTGATCTCAGGGAG ACAGAACAAGGATACAAAGGCCTCTCTATCCGGAGCGTGTGCTGGAAAGCAGACCGCCTTCTAGCAGGG ACCCAGGACAGTGAGATATTTGAAGTGATTGTGCGAGAGCGAGACAAGCCGATGTTGATCCTACAGGGC CACTGCGAGGGTGAGCTCTGGGCTCTGGCCCTGCACCCCAAGAAGCCTCTGGCTGTGACAGGCAGCGAT GACCGCTCTGTCAGGCTGTGGAGCCTGGCTGATCATGCCTTGATCGCCCGCTGTAACATGGAAGAGGCG GTTCGCAGTGTAGCTTTCAGCCCCGACGGATCTCAGCTGGCCCTGGGCATGAAGGACGGCTCTTTCATT GTTCTCCGAGTCAGAGATATGACAGAAGTAGTTCACATCAAAGATCGAAAAGAAGTCATTCATGAAATG AAATTTTCTCCAGATGGTTCTTACCTTGCAGTGGGATCCAATGATGGCCCAGTAGATGTCTATGCTGTT GCCCAGAGGTATAAGAAAATTGGAGAATGCAGCAAGTCCCTTAGTTTCATCACGCATATTGACTGGTCC TTGGATAGTAAATACTTACAAACTAATGACGGTGCAGGAGAACGATTGTTCTACAGAATGCCATCTGGG AAGCCTTTAACAAGTAAAGAAGAAATTAAAGGGATTCCTTGGGCCTCCTGGACATGCGTGAAAGGCCCT GAAGTGAGTGGAATTTGGCCCAAATACACTGAGGTTACTGACATCAACTCAGTGGATGCGAATTACAAC AGCTCAGTGCTGGTGTCTGGAGATGATTTTGGACTGGTTAAATTGTTTAAATTTCCTTGTCTCAAGAGA GGAGCCAAATTTAGAAAGTATGTGGGCCATTCTGCACATGTCACAAATGTCCGCTGGTCCCATGACTTT CAGTGGGTGTTGAGCACAGGAGGGGCTGATCACTCAGTTTTCCAGTGGAGGTTTATTCCAGAAGGTGTC AGCAACGGCATGCTGGAAACTGCACCCCAAGAAGGTGGAGCTGATTCCTACAGTGAAGAATCTGATTCA GATTTATCTGATGTGCCCGAACTGGACTCTGATATTGAGCAAGAAGCTCAAATCAATTATGATCGCCAG GTTTACAAAGAAGATCTACCTCAGCTAAAGCAACAAAGTAAAGAGAAAAACCACGCAGTGCCCTTCCTC AAACGAGAAAAGGCTCCTGAGGACAGCTTGAAACTCCAGTTCATACACGGTTATAGAGGCTACGACTGT AGAAACAATCTGTTCTACACACAAGCTGGAGAAGTAGTCTACCACATTGCTGCAGTTGCTGTCGTGTAT AATCGGCAGCAGCACTCCCAGAGGCTGTACCTGGGGCACGATGACGACATTCTCAGCCTGACCATCCAT CCAGTGAAGGACTATGTGGCTACTGGGCAGGTTGGAAGAGATGCTGCTATTCATGTGTGGGACACACAA ACTCTAAAATGTTTGTCGCTGTTGAAAGGACAACATCAGAGAGGAGTGTGTGCACTTGATTTTTCAGCC GATGGAAAATGTCTGGTGTCGGTTGGTTTAGACGATTTTCACAGTATTGTATTTTGGGACTGGAAAAAG GGAGAAAAGATAGCCACAACAAGAGGACATAAAGATAAGATATTTGTGGTAAAGTGTAACCCACACCAT GTTGACAAACTGGTTACAGTTGGGATAAAACACATCAAATTCTGGCAACAAGCAGGTGGGGGCTTCACT TCTAAAAGAGGAACTTTTGGAAGCGTTGGAAAATTGGAAACAATGATGTGTGTTTCTTACGGACGAATG GAAGATCTAGTGTTCTCAGGAGCAGCTACTGGAGATATTTTTATTTGGAAAGACATTCTACTACTGAAG ACAGTGAAAGCTCATGATGGGCCTGTGTTTGCTATGTATGCACTGGATAAGGGCTTTGTAACAGGTGGA AAGGACGGCATCGTGGAGCTCTGGGATGATATGTTTGAAAGATGTTTGAAGACTTATGCCATTAAAAGA TCAGCATTGTCGACTAGCTCAAAAGGCTTGCTTTTGGAAGATAACCCTTCAATTCGTGCCATCACTTTG GGACATGGACATATCCTGGTGGGAACAAAAAATGGAGAGATTCTGGAAATTGATAAGAGTGGCCCAATG ACACTGCTTGTTCAGGGGCACATGGAAGGAGAAGTGTGGGGGTTGGCAGCTCACCCTCTCCTGCCCATC TGTGCAACAGTGAGCGATGATAAAACACTTCGCATCTGGGAACTATCTGCCCAGCACCGTATGCTGGCA GTACGGAAACTCAAAAAAGGTGGAAGATGCTGTGCCTTTTCCCCTGATGGGAAAGCCTTAGCGGTTGGC TTGAACGATGGGAGTTTCCTGGTGGTAAATGCTGACACTGTTGAAGACATGGTCTCTTTCCATCACAGA AAAGAAATGATCTCTGATATTAAGTTTTCAAAAGATACGGGAAAATACCTTGCCGTGGCATCCCATGAT AACTTTGTGGATATTTACAACGTACTTACAAGCAAAAGGGTTGGCATCTGTAAAGGTGCTTCTAGTTAT ATTACACACATTGACTGGGACTCTAGAGGAAAATTATTGCAAGTGAATTCAGGTGCCAGAGAACAACTT TTTTTTGAAGCTCCAAGAGGCAAACGGCATATAATAAGACCTTCAGAGATCGAGAAGATAGAGTGGGAC ACATGGACCTGTGTCCTGGGGCCCACCTGTGAGGGAATCTGGCCAGCACATAGCGATATAACTGACGTA AATGCTGCCAGTCTTACCAAAGACTGTTCCCTTTTAGCCACCGGAGATGATTTTGGTTTCGTTAAGCTT TTTTCATATCCTGTCAAGGGACAGCACGCAAGATTCAAGAAGTATGTGGGGCACAGTGCACATGTCACT AACGTGAGGTGGCTGCACAATGACTCTGTGCTGCTCACGGTGGGCGGCGCCGACACAGCCCTGATGATC TGGACCAGGGAGTTTGTGGGGACCCAGGAGAGCAAGCTGGTGGACAGCGAGGAGTCAGACACCGACGTG GAAGAGGATGGAGGCTATGACAGCGATGTTGCTAGAGAAAAGGCCATTGACTACACCACCAAGATTTAT GCTGTGAGCATCAGGGAAATGGAAGGCACCAAGCCACACCAGCAGCTGAAGGAAGTTTCCGTGGAAGAA AGACCACCCGTTAGCCGAGCAGCTCCCCAGCCTGAGAAACTGCAGAAGAACAATATCACCAAAAAAAAG AAACTGGTTGAGGAGCTGGCTCTAGACCACGTGTTTGGCTACAGAGGTTTCGACTGTCGAAATAACCTG CATTACCTTAATGATGGCGCTGACATCATCTTCCACACAGCAGCGGCTGGCATCGTTCAGAACCTCTCC ACAGGGAGCCAGAGCTTCTATCTGGAGCACACAGATGACATCCTCTGTCTCACAGTGAACCAGCACCCC AAGTACAGAAACGTGGTGGCCACCAGCCAGATAGGGACAACACCTTCCATCCACATATGGGACGCCATG ACCAAACACACCCTCTCCATGCTGCGGTGCTTCCACTCCAAGGGGGTGAATTACATCAACTTCAGTGCA ACTGGAAAGCTCCTGGTGTCGGTGGGAGTGGACCCTGAGCACACCATCACTGTCTGGCGATGGCAGGAA GGTGCCAAGGTTGCCAGCCGAGGGGGTCACCTGGAGCGCATATTTGTGGTGGAATTTCGCCCCGACTCA GACACGCAGTTTGTATCTGTCGGGGTCAAACATATGAAGTTCTGGACCCTGGCAGGCAGCGCCTTGCTT TACAAGAAAGGGGTCATCGGGTCCCTGGGAGCTGCCAAAATGCAGACGATGCTCTCCGTGGCCTTCGGT GCTAACAATCTCACTTTCACGGGTGCCATCAATGGAGATGTCTACGTCTGGAAGGACCACTTCCTCATC CGGCTGGTGGCCAAGGCTCACACAGGCCCCGTGTTCACAATGTACACAACCCTTCGGGATGGACTCATA GTGACCGGCGGAAAAGAGAGGCCGACCAAAGAAGGAGGTGCTGTAAAATTGTGGGACCAGGAGATGAAG CGCTGCCGGGCCTTTCAGCTGGAGACCGGGCAGCTGGTGGAGTGTGTGCGCTCCGTGTGCCGTGGAAAA GGAAAAATCTTAGTGGGAACCAAAGACGGAGAAATAATTGAAGTTGGTGAAAAAAATGCTGCTTCTAAC ATCCTGATTGATGGTCACATGGAAGGGGAGATCTGGGGCCTGGCCACTCACCCTTCCAAGGACCTCTTC ATCTCTGCCAGCAACGATGGCACAGCCCGGATCTGGGACCTGGCTGACAAGAAGCTGTTAAACAAGGTG AGCTTGGGCCATGCGGCCAGGTGTGCAGCCTACAGCCCTGATGGGGAGATGGTGGCCATTGGCATGAAG AATGGAGAGTTTGTCATCTTGTTGGTGAACAGCCTGAAAGTTTGGGGGAAAAAACGAGACCGGAAATCT GCTATCCAAGATATCAGAATCAGCCCAGACAACCGATTCTTAGCCGTTGGTTCTTCTGAACACACAGTT GACTTCTATGACCTCACTCAGGGCACAAATCTGAACCGCATTGGCTACTGCAAAGATATCCCAAGCTTT GTCATTCAGATGGATTTTTCTGCGGATGGCAAATACATTCAGGTGTCAACAGGTGCCTATAAGCGCCAG GTGCATGAGGTCCCCCTGGGGAAGCAGGTAACTGAAGCCGTGGTCATTGAGAAGATCACCTGGGCCTCC TGGACAAGCGTCCTGGGAGATGAAGTCATTGGAATCTGGCCACGAAATGCAGACAAGGCTGATGTCAAC TGCGCATGTGTGACCCACGCTGGCCTGAACATTGTCACAGGAGATGACTTTGGGCTGGTGAAGCTCTTT GATTTTCCATGCACAGAAAAATTTGCCAAACATAAGCGATACTTCGGTCACTCGGCTCACGTGACGAAC ATCCGTTTCTCTTATGATGACAAGTATGTGGTCAGCACTGGAGGAGACGACTGCAGTGTATTTGTGTGG CGATGTCTGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001039753
Insert Size	5877 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001039753.2
RefSeq Size	7853 bp
RefSeq ORF	5877 bp
Locus ID	400954
UniProt ID	Q6ZMW3
MW	217.9 kDa
Gene Summary	May modify the assembly dynamics of microtubules, such that microtubules are slightly longer, but more dynamic.[UniProtKB/Swiss-Prot Function]

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