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USP20 (NM_001008563) Human Untagged Clone
CAT＃: SC328026
USP20 (untagged)-Human ubiquitin specific peptidase 20 (USP20) transcript variant 2
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | hVDU2; LSFR3A; VDU2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC328026 representing NM_001008563.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGGGGACTCCAGGGACCTTTGCCCTCACCTTGACTCCATAGGAGAGGTGACCAAAGAGGACTTGCTG CTCAAATCTAAGGGAACCTGTCAGTCGTGTGGGGTCACCGGACCAAACCTATGGGCCTGTCTGCAGGTT GCCTGCCCCTATGTTGGCTGCGGAGAATCCTTTGCTGACCACAGCACCATTCATGCACAGGCAAAAAAG CACAACTTGACCGTGAACCTGACCACGTTCCGACTGTGGTGTTACGCCTGTGAGAAGGAGGTATTCCTG GAGCAGCGGCTGGCAGCCCCTCTGCTGGGCTCCTCTTCCAAGTTCTCTGAACAGGACTCCCCGCCACCC TCCCACCCTCTGAAAGCTGTTCCTATTGCTGTGGCTGATGAAGGAGAGTCTGAGTCAGAGGACGATGAC CTGAAACCTCGAGGCCTCACGGGCATGAAGAACCTCGGGAACTCCTGCTACATGAACGCTGCCCTGCAG GCCCTGTCCAATTGCCCGCCGCTGACTCAGTTCTTCTTGGAGTGTGGCGGCCTGGTGCGCACAGATAAG AAGCCAGCCCTGTGCAAGAGCTACCAGAAGCTGGTCTCTGAGGTCTGGCATAAGAAACGGCCAAGCTAC GTGGTCCCCACCAGTCTGTCTCATGGGATCAAGTTGGTCAACCCAATGTTCCGAGGCTATGCCCAGCAG GACACCCAAGAGTTCCTTCGCTGCCTGATGGACCAGCTGCACGAGGAGCTCAAGGAGCCGGTGGTGGCC ACGGTGGCGCTGACGGAGGCTCGGGACTCAGATTCGAGTGACACGGATGAGAAACGGGAGGGTGACCGG AGCCCATCAGAAGATGAGTTCTTGTCCTGTGACTCGAGCAGTGACCGGGGTGAGGGTGACGGGCAGGGG CGTGGCGGGGGCAGCTCGCAGGCCGAGACGGAGCTGCTGATCCCAGATGAGGCGGGCCGAGCCATCTCT GAGAAGGAGCGGATGAAGGACCGCAAGTTCTCCTGGGGCCAGCAGCGTACAAACTCGGAGCAAGTGGAC GAGGACGCTGATGTGGACACTGCCATGGCTGCCCTTGACGACCAGCCCGCGGAGGCCCAGCCCCCGTCA CCACGGTCCTCCAGCCCCTGCCGGACGCCAGAGCCGGACAATGATGCTCACCTACGCAGCTCCTCTCGC CCCTGCAGCCCCGTCCACCACCACGAGGGCCATGCCAAGCTGTCTAGCAGCCCCCCTCGTGCAAGCCCC GTGAGGATGGCACCGTCGTACGTGCTCAAGAAAGCCCAGGTATTGAGTGCTGGCAGCCGGAGGCGGAAG GAGCAGCGCTACCGCAGCGTCATCTCAGACATCTTTGACGGCTCCATTCTCAGCCTTGTGCAGTGTCTC ACCTGTGACCGGGTATCCACCACAGTGGAAACGTTCCAGGACTTATCACTGCCCATTCCTGGAAAGGAG GACCTGGCCAAGCTCCATTCAGCCATCTACCAGAATGTGCCGGCCAAGCCAGGCGCCTGTGGGGACAGC TATGCCGCCCAGGGCTGGCTGGCCTTCATTGTGGAGTACATCCGACGGTTTGTGGTATCCTGTACCCCC AGCTGGTTTTGGGGGCCTGTCGTCACCCTGGAAGACTGCCTTGCTGCCTTCTTTGCCGCTGATGAGTTA AAGGGTGACAACATGTACAGCTGTGAGCGGTGTAAGAAGCTGCGGAACGGAGTGAAGTACTGCAAAGTC CTGCGGTTGCCCGAGATCCTGTGCATTCACCTAAAGCGCTTTCGGCACGAGGTGATGTACTCATTCAAG ATCAACAGCCACGTCTCCTTCCCCCTCGAGGGGCTCGACCTGCGCCCCTTCCTTGCCAAGGAGTGCACA TCCCAGATCACCACCTACGACCTCCTCTCGGTCATCTGCCACCACGGCACGGCAGGCAGTGGGCACTAC ATCGCCTACTGCCAGAACGTGATCAATGGGCAGTGGTACGAGTTTGATGACCAGTACGTCACAGAAGTC CACGAGACGGTGGTGCAGAACGCCGAGGGCTACGTACTCTTCTACAGGAAGAGCAGCGAGGAGGCCATG CGGGAGCGACAGCAGGTGGTGTCCCTGGCCGCCATGCGGGAGCCCAGCCTGCTGCGGTTCTACGTGTCC CGCGAGTGGCTCAACAAGTTCAACACCTTCGCGGAGCCAGGCCCCATCACCAACCAGACCTTCCTCTGC TCCCACGGAGGCATCCCGCCCCACAAATACCACTACATCGACGACCTGGTGGTCATCCTGCCCCAGAAC GTCTGGGAGCACCTGTACAACAGATTCGGGGGTGGCCCCGCCGTGAACCACCTGTACGTGTGCTCCATC TGCCAGGTGGAGATCGAGGCACTGGCCAAGCGCAGGAGGATCGAGATCGACACCTTCATCAAGTTGAAC AAGGCCTTCCAGGCCGAGGAGTCGCCGGGCGTCATCTACTGCATCAGCATGCAGTGGTTCCGGGAGTGG GAGGCGTTCGTCAAGGGGAAGGACAACGAGCCCCCCGGGCCCATTGACAACAGCAGGATTGCACAGGTC AAAGGAAGCGGCCATGTCCAGCTGAAGCAGGGAGCTGACTACGGGCAGATTTCGGAGGAGACCTGGACC TACCTGAACAGCCTGTATGGAGGTGGCCCCGAGATTGCCATCCGCCAGAGTGTGGCGCAGCCGCTGGGC CCAGAGAACCTGCACGGGGAGCAGAAGATCGAAGCCGAGACGCGGGCCGTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001008563 |
| Insert Size | 2745 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001008563.4 |
| RefSeq Size | 4459 bp |
| RefSeq ORF | 2745 bp |
| Locus ID | 10868 |
| UniProt ID | Q9Y2K6 |
| Protein Families | Druggable Genome, Protease |
| MW | 102 kDa |
| Gene Summary | This gene encodes a ubiquitin specific processing protease that was first identified as a substrate of the VHL (von Hippel-Lindau disease) protein E3 ubiquitin ligase complex. In addition to being ubiquitinated by the VHL-E3 ligase complex, this enzyme deubiquitinates hypoxia-inducible factor (HIF)-1 alpha and thereby causes increased expression of HIF-1alpha targeted genes which play a role in angiogenesis, glucose metabolism, cell proliferation and metastasis. The enzyme encoded by this gene also regulates G-protein coupled receptor signaling by mediating the deubiquitination of beta-2 adrenergic receptor (ADRB2). This enzyme is a ubiquitously expressed thiolester hydrolase. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jan 2013] Transcript Variant: This variant (2) has different 5' and 3' non-coding, terminal exons, compared to transcript variant 1. All three transcript variants encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments and orthologous data. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC213090 | USP20 (Myc-DDK-tagged)-Human ubiquitin specific peptidase 20 (USP20), transcript variant 2 |
CNY 3,990.00 |
|
| RC213090L3 | Lenti ORF clone of Human ubiquitin specific peptidase 20 (USP20), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC213090L4 | Lenti ORF clone of Human ubiquitin specific peptidase 20 (USP20), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG213090 | USP20 (tGFP-tagged) - Human ubiquitin specific peptidase 20 (USP20), transcript variant 2 |
CNY 4,370.00 |
|
| SC301324 | USP20 (untagged)-Human ubiquitin specific peptidase 20 (USP20), transcript variant 2 |
CNY 7,220.00 |
