CRY2 (NM_021117) Human Untagged Clone

CAT#: SC327944

CRY2 (untagged)-Human cryptochrome 2 (photolyase-like) (CRY2) transcript variant 1



  "NM_021117" in other vectors (9)

CNY 9,880.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
CRY2 mouse monoclonal antibody, clone OTI1H5 (formerly 1H5)
    • 100 ul

CNY 1,999.00
CNY 2,700.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms HCRY2; PHLL2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>SC327944 representing NM_021117.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGGCGGGGTCCACGTCGCCTACCGGGGCGGAGCGGGGGTGGCTGGAGCAGTCTGGACAGTCATGGCG
GCGACTGTGGCGACGGCGGCAGCTGTGGCCCCGGCGCCAGCGCCCGGCACGGACAGCGCCTCTTCGGTG
CACTGGTTCCGCAAAGGGCTGCGACTCCACGACAACCCGGCGTTGCTGGCGGCCGTGCGCGGGGCGCGC
TGCGTGCGCTGCGTTTACATTCTCGACCCGTGGTTCGCGGCCTCCTCCTCAGTCGGGATCAACCGATGG
AGGTTCCTACTTCAGTCTCTGGAAGATTTGGACACAAGTTTAAGGAAACTGAACTCCCGCCTGTTTGTA
GTCCGGGGACAGCCAGCCGACGTGTTCCCAAGGCTGTTCAAGGAATGGGGAGTGACCCGCTTGACCTTT
GAATATGACTCTGAACCCTTTGGGAAAGAACGGGATGCAGCCATCATGAAGATGGCCAAGGAGGCTGGT
GTGGAAGTAGTGACGGAGAATTCTCATACCCTCTATGACCTGGACAGGATCATTGAGCTGAATGGGCAG
AAGCCACCCCTTACATACAAGCGCTTTCAGGCCATCATCAGCCGCATGGAGCTGCCCAAGAAGCCAGTG
GGCTTGGTGACCAGCCAGCAGATGGAGAGCTGCAGGGCCGAGATCCAGGAGAACCACGACGAGACCTAC
GGCGTGCCCTCCCTGGAGGAGCTGGGGTTCCCCACTGAAGGACTTGGTCCAGCTGTCTGGCAGGGAGGA
GAGACAGAAGCTCTGGCCCGCCTGGATAAGCACTTGGAACGGAAGGCCTGGGTTGCCAACTATGAGAGA
CCCCGAATGAACGCCAACTCCCTCCTGGCCAGCCCCACAGGCCTCAGCCCCTACCTGCGCTTTGGTTGT
CTCTCCTGCCGCCTCTTCTACTACCGCCTGTGGGACCTGTATAAAAAGGTGAAGCGGAACAGCACACCT
CCCCTCTCCCTATTTGGGCAACTCCTATGGCGAGAGTTCTTCTACACGGCAGCTACCAACAACCCCAGG
TTTGACCGCATGGAGGGGAACCCCATCTGCATCCAGATCCCCTGGGACCGCAATCCTGAGGCCCTGGCC
AAGTGGGCTGAGGGCAAGACAGGCTTCCCTTGGATTGATGCCATCATGACCCAACTGAGGCAGGAGGGC
TGGATCCACCACCTGGCCCGGCATGCCGTGGCCTGCTTCCTGACCCGCGGGGACCTCTGGGTCAGCTGG
GAGAGCGGGGTCCGGGTATTTGATGAGCTGCTCCTGGATGCAGATTTCAGCGTGAACGCAGGCAGCTGG
ATGTGGCTGTCCTGCAGTGCTTTCTTCCAGCAGTTCTTCCACTGCTACTGCCCTGTGGGCTTTGGCCGT
CGCACGGACCCCAGTGGGGACTACATCAGGCGATACCTGCCCAAATTGAAAGCGTTCCCCTCTCGATAC
ATCTATGAGCCCTGGAATGCCCCAGAGTCAATTCAGAAGGCAGCCAAGTGCATCATTGGTGTGGACTAC
CCACGGCCCATCGTCAACCATGCCGAGACCAGCCGGCTTAACATTGAACGAATGAAGCAGATTTACCAG
CAGCTTTCGCGCTACCGGGGACTCTGTCTACTGGCATCTGTCCCTTCCTGTGTGGAAGACCTCAGTCAC
CCTGTGGCAGAGCCCAGCTCGAGCCAGGCTGGCAGCATGAGCAGTGCAGGCCCAAGACCACTACCCAGT
GGCCCAGCATCCCCCAAACGCAAGCTGGAAGCAGCCGAGGAACCACCTGGTGAAGAACTCAGCAAACGG
GCCCGGGTGGCAGAGTTGCCAACCCCAGAGCTGCCGAGCAAGGATGCCTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI     
ACCN NM_021117
Insert Size 1845 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_021117.3
RefSeq Size 4204 bp
RefSeq ORF 1845 bp
Locus ID 1408
UniProt ID Q49AN0
Protein Families Druggable Genome
Protein Pathways Circadian rhythm - mammal
MW 68.9 kDa
Gene Summary This gene encodes a flavin adenine dinucleotide-binding protein that is a key component of the circadian core oscillator complex, which regulates the circadian clock. This gene is upregulated by CLOCK/ARNTL heterodimers but then represses this upregulation in a feedback loop using PER/CRY heterodimers to interact with CLOCK/ARNTL. Polymorphisms in this gene have been associated with altered sleep patterns. The encoded protein is widely conserved across plants and animals. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2014]
Transcript Variant: This variant (1) uses an alternate first exon which contains the predicted translation start site; the resulting protein has a distinct N-terminus. CCDS Note: The coding region has been updated to shorten the N-terminus to one that is more supported by conservation.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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