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COASY (NM_001042532) Human Untagged Clone
CAT＃: SC327934
COASY (untagged)-Human Coenzyme A synthase (COASY) nuclear gene encoding mitochondrial protein transcript variant 4
CNY 9,600.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DPCK; NBIA6; NBP; PCH12; pOV-2; PPAT; UKR1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC327934 representing NM_001042532.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAGGACACCAAGGCTTAGAGCACAGCCCCGAGGCGCCGTCTACCAGGCCCCGTCCCCTCCCCCGGCT CCTGTCGGCCTGGGCAGCATGGCCGTATTCCGGTCGGGTCTCCTGGTGCTGACGACGCCGCTGGCCTCC CTAGCCCCTCGCCTGGCCTCCATCCTGACCTCGGCGGCCCGGCTGGTGAATCACACACTCTATGTTCAC CTGCAGCCGGGCATGAGCCTGGAGGGCCCGGCTCAGCCCCAGTCCAGCCCCGTGCAGGCCACGTTTGAG GTTCTTGATTTCATCACGCACCTCTATGCTGGCGCCGACGTCCACAGGCACTTGGACGTCAGAATCCTA CTGACCAATATCCGAACCAAGAGCACCTTTCTCCCTCCCCTGCCCACCTCAGTCCAGAATCTCGCCCAC CCGCCAGAAGTCGTGTTGACAGATTTCCAGACCCTGGATGGAAGCCAGTACAACCCGGTCAAACAGCAG CTAGTGCGTTACGCCACCAGCTGTTACAGCTGTTGTCCGCGACTGGCCTCGGTGCTGCTATACTCCGAT TATGGGATAGGAGAAGTGCCCGTGGAGCCCCTGGATGTCCCCTTACCCTCCACGATCAGGCCAGCTTCC CCCGTGGCCGGGTCTCCAAAGCAGCCGGTGCGTGGCTACTACCGTGGCGCTGTCGGTGGCACGTTTGAC CGCCTGCACAACGCCCACAAGGTGTTGCTCAGTGTCGCGTGCATCCTGGCCCAGGAGCAGCTTGTGGTG GGAGTAGCAGACAAAGATCTGTTGAAGAGCAAGTTGCTCCCTGAGCTGCTCCAACCTTATACAGAACGT GTGGAACATCTGAGTGAATTCCTGGTGGACATCAAGCCCTCCTTGACTTTTGATGTCATCCCCCTGCTG GACCCCTATGGGCCCGCTGGCTCTGACCCCTCCCTGGAGTTCCTGGTGGTCAGCGAGGAGACCTATCGT GGGGGGATGGCCATCAACCGCTTCCGCCTTGAGAATGACCTGGAGGAACTTGCTTTGTACCAGATCCAG CTGCTGAAGGACCTCAGACATACAGAGAATGAAGAGGACAAAGTCAGCTCCTCCAGCTTCCGCCAGCGA ATGTTGGGGAACCTGCTTCGGCCTCCATATGAAAGGCCAGAGCTCCCCACATGTCTCTATGTAATTGGG CTGACTGGCATCAGTGGCTCTGGGAAGAGCTCAATAGCTCAGCGACTGAAGGGCCTGGGGGCGTTTGTC ATTGACAGTGACCACCTGGGTCATCGGGCCTATGCCCCAGGTGGCCCTGCCTACCAGCCTGTGGTGGAG GCCTTTGGAACAGATATTCTCCATAAAGATGGCATCATCAACAGGAAGGTCCTAGGCAGCCGGGTGTTT GGGAATAAGAAGCAGCTGAAGATACTCACGGACATTATGTGGCCAATTATCGCAAAGCTGGCCCGAGAG GAGATGGATCGGGCTGTGGCTGAGGGAAAGCGTGTGTGTGTGATTGATGCCGCTGTGTTGCTTGAAGCC GGCTGGCAGAACCTGGTCCATGAGGTGTGGACTGCTGTCATCCCAGAGACTGAGGCTGTAAGACGCATT GTGGAGAGGGATGGCCTCAGTGAAGCCGCGGCTCAAAGCCGGCTGCAGAGCCAGATGAGCGGGCAGCAG CTTGTGGAACAGAGCCACGTGGTGCTCAGCACCTTGTGGGAGCCGCATATCACCCAACGCCAGGTGGAG AAAGCCTGGGCCCTCTTGCAGAAGCGCATTCCCAAGACTCATCAGGCCCTCGACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001042532 |
| Insert Size | 1782 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001042532.3 |
| RefSeq Size | 2314 bp |
| RefSeq ORF | 1782 bp |
| Locus ID | 80347 |
| UniProt ID | Q13057 |
| Protein Pathways | Metabolic pathways, Pantothenate and CoA biosynthesis |
| MW | 65.3 kDa |
| Gene Summary | Coenzyme A (CoA) functions as a carrier of acetyl and acyl groups in cells and thus plays an important role in numerous synthetic and degradative metabolic pathways in all organisms. In eukaryotes, CoA and its derivatives are also involved in membrane trafficking and signal transduction. This gene encodes the bifunctional protein coenzyme A synthase (CoAsy) which carries out the last two steps in the biosynthesis of CoA from pantothenic acid (vitamin B5). The phosphopantetheine adenylyltransferase domain of this bifunctional protein catalyzes the conversion of 4'-phosphopantetheine into dephospho-coenzyme A (dpCoA) while its dephospho-CoA kinase domain completes the final step by phosphorylating dpCoA to form CoA. Mutations in this gene are associated with neurodegeneration with brain iron accumulation (NBIA). Alternative splicing results in multiple isoforms. [provided by RefSeq, Apr 2014] Transcript Variant: This variant (4) differs in the 5' UTR and contains an additional segment in the 5' coding region, compared to variant 1. The resulting isoform (c, also known as CoASy beta) is longer at the N-terminus, compared to isoform a. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC229299 | COASY (Myc-DDK-tagged)-Human CoA synthase (COASY), nuclear gene encoding mitochondrial protein, transcript variant 4 |
CNY 4,416.00 |
|
| RC229299L3 | Lenti-ORF clone of COASY (Myc-DDK-tagged)-Human CoA synthase (COASY), nuclear gene encoding mitochondrial protein, transcript variant 4 |
CNY 6,460.00 |
|
| RC229299L4 | Lenti-ORF clone of COASY (mGFP-tagged)-Human CoA synthase (COASY), nuclear gene encoding mitochondrial protein, transcript variant 4 |
CNY 6,460.00 |
|
| RG229299 | COASY (tGFP-tagged) - Human CoA synthase (COASY), nuclear gene encoding mitochondrial protein, transcript variant 4 |
CNY 5,040.00 |
|
| SC311397 | COASY (untagged)-Human CoA synthase (COASY), nuclear gene encoding mitochondrial protein, transcript variant 4 |
CNY 9,600.00 |
